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- W4386832979 abstract "The gut and intestinal microbiota, consisting of trillions of microorganisms inhabiting the human gastrointestinal tract. It plays a crucial role in human health leading to understanding the dynamic crosstalk of host-microbe interaction in the gut and has become necessary for the detection, prevention, or therapy of diseases. Gut microbiota deviations are linked with many diseases, suggesting that various pathways involved in immunity, energy, lipid, and glucose metabolism are affected. Further, it is also altered by external insults such as metal toxicity, antibiotics and pesticides. Heavy metals like arsenic, mercury, cadmium and chromium are one of the well-studied classes of environmental pollutants. Mouse models have become the model of choice for most studies in this emerging field, as they allow perturbations in the gut microbiota to be studied in a controlled experimental setup. Here, we investigate the composition and diversity of intestinal microbes utilizing cecal samples from different intervention groups: arsenic exposure (As(III)), arsenic and piperine co-administration (As+Pp), piperine perse and control group. We obtained DNA samples from these groups and performed PCR amplification and sequencing of the 16S V3-V4 region. The sequencing data were analyzed utilizing bioinformatics tools and taxonomically categorized. The findings showed shift in microbial composition and abundance among different intervention groups, revealing taxa that may contribute to the microbial diversity." @default.
- W4386832979 created "2023-09-19" @default.
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- W4386832979 date "2023-09-16" @default.
- W4386832979 modified "2023-09-26" @default.
- W4386832979 title "Editor response for version 1" @default.
- W4386832979 doi "https://doi.org/10.1099/acmi.0.000653.v1.5" @default.
- W4386832979 hasPublicationYear "2023" @default.
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