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- W4386840417 abstract "Botryosphaeriaceous fungi are widespread, and cause serious diseases in many economically important crops. Botryosphaeria dothidea, Neofusicoccum mediterraneum and N. parvum are the most important members of this family in the Mediterranean region. These fungi are frequently isolated from the same host, which together with their extensive and increasing host range necessitates development of rapid and reliable diagnostic tools. Species boundaries within the Botryosphaeriaceae have been defined based on phylogenetic analyses of multiple gene sequences, including those of mating type genes. The MAT1-2-1 gene displayed high sequence variability between Botryosphaeriaceous species, so was selected as the target for development of a definitive diagnostic tool. This paper outlines a new and robust molecular tool, composed of three TaqMan assays based on polymorphisms located in the MAT1-2-1 gene of B. dothidea, N. mediterraneum and N. parvum. Each assay differentiated the target species from other Botryosphaeriaceae, and from non-target fungi." @default.
- W4386840417 created "2023-09-19" @default.
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- W4386840417 date "2023-07-23" @default.
- W4386840417 modified "2023-09-27" @default.
- W4386840417 title "Dual labelled probe assays for differentiation of Botryosphaeria dothidea, Neofusicoccum mediterraneum and Neofusicoccum parvum, based on polymorphisms in the MAT1-2-1 gene" @default.
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- W4386840417 doi "https://doi.org/10.36253/phyto-14375" @default.
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