Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386859372> ?p ?o ?g. }
- W4386859372 abstract "Alternative splicing regulates gene expression and functional diversity and is often dysregulated in human cancers. Here, we discovered that the long noncoding RNA (lncRNA) MIR99AHG regulated alternative splicing to alter the activity of a chromatin remodeler and promote metastatic behaviors in colorectal cancer (CRC). MIR99AHG was abundant in invasive CRC cells and metastatic tumors from patients and promoted motility and invasion in cultured CRC cells. MIR99AHG bound to and stabilized the RNA splicing factor PTBP1, and this complex increased cassette exon inclusion in the mRNA encoding the chromatin remodeling gene SMARCA1 . Specifically, MIR99AHG altered the nature of PTBP1 binding to the splice sites on intron 12 of SMARCA1 pre-mRNA, thereby triggering a splicing switch from skipping to including exon 13 to produce the long isoform, SMARCA1-L. SMARCA1, but not SMARCA1-L, suppressed invadopodia formation, cell migration, and invasion. Analysis of CRC samples revealed that the abundance of MIR99AHG transcript positively correlated with that of SMARCA1-L mRNA and PTBP1 protein and with poor prognosis in patients with CRC. Furthermore, TGF-β1 secretion from cancer-associated fibroblasts increased MIR99AHG expression in CRC cells. Our findings identify an lncRNA that is induced by cues from the tumor microenvironment and that interacts with PTBP1 to regulate alternative splicing, potentially providing a therapeutic target and predictive biomarker for metastatic CRC." @default.
- W4386859372 created "2023-09-20" @default.
- W4386859372 creator A5007247087 @default.
- W4386859372 creator A5007966510 @default.
- W4386859372 creator A5008651022 @default.
- W4386859372 creator A5019942906 @default.
- W4386859372 creator A5023229396 @default.
- W4386859372 creator A5024329209 @default.
- W4386859372 creator A5030406251 @default.
- W4386859372 creator A5031340473 @default.
- W4386859372 creator A5037677450 @default.
- W4386859372 creator A5044695931 @default.
- W4386859372 creator A5046054581 @default.
- W4386859372 creator A5054624830 @default.
- W4386859372 creator A5055801372 @default.
- W4386859372 creator A5057840497 @default.
- W4386859372 creator A5061852170 @default.
- W4386859372 creator A5062616205 @default.
- W4386859372 creator A5069079825 @default.
- W4386859372 creator A5074266150 @default.
- W4386859372 creator A5078303906 @default.
- W4386859372 creator A5080177287 @default.
- W4386859372 creator A5080821647 @default.
- W4386859372 creator A5082030295 @default.
- W4386859372 creator A5083567233 @default.
- W4386859372 creator A5085028455 @default.
- W4386859372 creator A5087167187 @default.
- W4386859372 creator A5088664989 @default.
- W4386859372 creator A5090617575 @default.
- W4386859372 date "2023-09-19" @default.
- W4386859372 modified "2023-10-16" @default.
- W4386859372 title "The lncRNA MIR99AHG directs alternative splicing of <i>SMARCA1</i> by PTBP1 to enable invadopodia formation in colorectal cancer cells" @default.
- W4386859372 cites W1963501822 @default.
- W4386859372 cites W1967778417 @default.
- W4386859372 cites W1974898488 @default.
- W4386859372 cites W1985143502 @default.
- W4386859372 cites W1988787904 @default.
- W4386859372 cites W2018114690 @default.
- W4386859372 cites W2027260842 @default.
- W4386859372 cites W2028198233 @default.
- W4386859372 cites W2042086006 @default.
- W4386859372 cites W2058781335 @default.
- W4386859372 cites W2097572021 @default.
- W4386859372 cites W2116432038 @default.
- W4386859372 cites W2117116688 @default.
- W4386859372 cites W2117691023 @default.
- W4386859372 cites W2122358139 @default.
- W4386859372 cites W2122683221 @default.
- W4386859372 cites W2130410032 @default.
- W4386859372 cites W2136785472 @default.
- W4386859372 cites W2153268494 @default.
- W4386859372 cites W2154241726 @default.
- W4386859372 cites W2165200432 @default.
- W4386859372 cites W2166566864 @default.
- W4386859372 cites W2167786463 @default.
- W4386859372 cites W2260715119 @default.
- W4386859372 cites W2395807466 @default.
- W4386859372 cites W2505384565 @default.
- W4386859372 cites W2555357497 @default.
- W4386859372 cites W2560385907 @default.
- W4386859372 cites W2586630468 @default.
- W4386859372 cites W2594091455 @default.
- W4386859372 cites W2605986786 @default.
- W4386859372 cites W2614855056 @default.
- W4386859372 cites W2766837662 @default.
- W4386859372 cites W2769407685 @default.
- W4386859372 cites W2883929619 @default.
- W4386859372 cites W2889104271 @default.
- W4386859372 cites W2906076922 @default.
- W4386859372 cites W2914485122 @default.
- W4386859372 cites W2934231425 @default.
- W4386859372 cites W2944157576 @default.
- W4386859372 cites W2945489918 @default.
- W4386859372 cites W2970033143 @default.
- W4386859372 cites W2970045605 @default.
- W4386859372 cites W2980949872 @default.
- W4386859372 cites W3006556144 @default.
- W4386859372 cites W3011137119 @default.
- W4386859372 cites W3011709397 @default.
- W4386859372 cites W3109804732 @default.
- W4386859372 cites W3115880421 @default.
- W4386859372 cites W3139737861 @default.
- W4386859372 cites W3159017136 @default.
- W4386859372 cites W3159024074 @default.
- W4386859372 cites W3163092889 @default.
- W4386859372 cites W3209656321 @default.
- W4386859372 cites W4295900651 @default.
- W4386859372 cites W4312109262 @default.
- W4386859372 doi "https://doi.org/10.1126/scisignal.adh4210" @default.
- W4386859372 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37725664" @default.
- W4386859372 hasPublicationYear "2023" @default.
- W4386859372 type Work @default.
- W4386859372 citedByCount "0" @default.
- W4386859372 crossrefType "journal-article" @default.
- W4386859372 hasAuthorship W4386859372A5007247087 @default.
- W4386859372 hasAuthorship W4386859372A5007966510 @default.
- W4386859372 hasAuthorship W4386859372A5008651022 @default.
- W4386859372 hasAuthorship W4386859372A5019942906 @default.
- W4386859372 hasAuthorship W4386859372A5023229396 @default.
- W4386859372 hasAuthorship W4386859372A5024329209 @default.