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• W4386861142 abstract "Electrospray ionization (ESI) is one of the main techniques used in mass spectrometry (MS) of nonvolatile compounds. ESI is a disordered process, in which a large number of polydisperse droplets are projected from a fluctuating Taylor cone and jet protruding ESI emitter. Here, we disclose a system for sectioning electrospray plumes to discrete packets with millisecond and submillisecond lifetime, which are introduced to the MS orifice, one at a time. A high-speed camera was triggered at 10,000 frames per second to capture consecutive images of the electrospray packets transmitted to the mass spectrometer. We further correlated the high-speed images of electrospray packets with MS signals of a test analyte (acetaminophen). Following computational treatment of the images, we determined the number of droplet observations (<300), average diameter of droplets (∼10-20 μm), and average volume of droplets (few tens of picoliters) in the individual electrospray packets. The result shows that most micrometer droplets (>10 μm) do not have any significant contribution to the MS signals. This finding is in agreement with the prior conjecture that most of the MS signals are mainly attributed to nanodroplets. Based on this finding, one can deduce that only a small number of the initial microdroplets effectively carry analyte molecules that undergo ionization. We discuss that, in future, one may propose a way to recharge the emitted initial micrometer droplets to increase the efficiency of conventional ESI setups." @default.
• W4386861142 created "2023-09-20" @default.
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• W4386861142 date "2023-09-19" @default.
• W4386861142 modified "2023-10-14" @default.
• W4386861142 title "Are Most Micrometer Droplets (>10 μm) Wasted in Electrospray Ionization? An Insight from Real-Time High-Speed Imaging" @default.
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• W4386861142 doi "https://doi.org/10.1021/acs.analchem.3c02799" @default.
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• W4386861142 hasPublicationYear "2023" @default.
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