Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386864100> ?p ?o ?g. }
- W4386864100 endingPage "890" @default.
- W4386864100 startingPage "890" @default.
- W4386864100 abstract "Cytochrome c (Cytc) is a key redox protein for energy metabolism and apoptosis in cells. The activation of Cytc is composed of several steps, including its transfer to the mitochondrial membrane, binding to cytochrome c heme lyase (CCHL) and covalent attachment to heme. The spectroscopic methods are often applied to study the structural changes of Cytc. However, they require the isolation of Cytc from cells and have limited availability under physiological conditions. Despite recent studies to elucidate the tightly regulated folding mechanism of Cytc, the role of these events and their association with different conformational states remain elusive. Here, we provide a genetically encoded fluorescence method that allows monitoring of the conformational changes of Cytc upon binding to heme and CCHL. Cerulean and Venus fluorescent proteins attached at the N and C terminals of Cytc can be used to determine its unfolded, intermediate, and native states by measuring FRET amplitude. We found that the noncovalent interaction of heme in the absence of CCHL induced a shift in the FRET signal, indicating the formation of a partially folded state. The higher concentration of heme and coexpression of CCHL gave rise to the recovery of Cytc native structure. We also found that Cytc was weakly associated with CCHL in the absence of heme. As a result, a FRET-based fluorescence approach was demonstrated to elucidate the mechanism of heme-induced Cytc conformational changes with spatiotemporal resolution and can be applied to study its interaction with small molecules and other protein partners in living cells." @default.
- W4386864100 created "2023-09-20" @default.
- W4386864100 creator A5006937058 @default.
- W4386864100 creator A5061206817 @default.
- W4386864100 creator A5072012555 @default.
- W4386864100 creator A5092902810 @default.
- W4386864100 date "2023-09-18" @default.
- W4386864100 modified "2023-10-17" @default.
- W4386864100 title "Genetically Encoded Fluorescent Probe for Detection of Heme-Induced Conformational Changes in Cytochrome c" @default.
- W4386864100 cites W1244643824 @default.
- W4386864100 cites W1490917634 @default.
- W4386864100 cites W1507369121 @default.
- W4386864100 cites W1599863157 @default.
- W4386864100 cites W1939801300 @default.
- W4386864100 cites W1966587300 @default.
- W4386864100 cites W1971874990 @default.
- W4386864100 cites W1989082315 @default.
- W4386864100 cites W1991611640 @default.
- W4386864100 cites W1992974234 @default.
- W4386864100 cites W1993387044 @default.
- W4386864100 cites W1996880521 @default.
- W4386864100 cites W2000567066 @default.
- W4386864100 cites W2000733866 @default.
- W4386864100 cites W2001626642 @default.
- W4386864100 cites W2002621746 @default.
- W4386864100 cites W2025866005 @default.
- W4386864100 cites W2026315032 @default.
- W4386864100 cites W2032881207 @default.
- W4386864100 cites W2042338072 @default.
- W4386864100 cites W2057699425 @default.
- W4386864100 cites W2065200208 @default.
- W4386864100 cites W2073112261 @default.
- W4386864100 cites W2075336700 @default.
- W4386864100 cites W2086318858 @default.
- W4386864100 cites W2086405618 @default.
- W4386864100 cites W2089717957 @default.
- W4386864100 cites W2090499415 @default.
- W4386864100 cites W2090970108 @default.
- W4386864100 cites W2093369561 @default.
- W4386864100 cites W2107382158 @default.
- W4386864100 cites W2109683405 @default.
- W4386864100 cites W2111475099 @default.
- W4386864100 cites W2115138779 @default.
- W4386864100 cites W2127798977 @default.
- W4386864100 cites W2131995085 @default.
- W4386864100 cites W2135979687 @default.
- W4386864100 cites W2142379241 @default.
- W4386864100 cites W2154298653 @default.
- W4386864100 cites W2155345385 @default.
- W4386864100 cites W2161384956 @default.
- W4386864100 cites W2162204497 @default.
- W4386864100 cites W2162390197 @default.
- W4386864100 cites W2210811658 @default.
- W4386864100 cites W2288318116 @default.
- W4386864100 cites W2295658693 @default.
- W4386864100 cites W2301795778 @default.
- W4386864100 cites W2314200992 @default.
- W4386864100 cites W2317873538 @default.
- W4386864100 cites W2335741006 @default.
- W4386864100 cites W2415954632 @default.
- W4386864100 cites W2419385546 @default.
- W4386864100 cites W2437577115 @default.
- W4386864100 cites W2503729860 @default.
- W4386864100 cites W2603025173 @default.
- W4386864100 cites W2699490391 @default.
- W4386864100 cites W2762968408 @default.
- W4386864100 cites W2767021870 @default.
- W4386864100 cites W2767529428 @default.
- W4386864100 cites W2791895358 @default.
- W4386864100 cites W2801869326 @default.
- W4386864100 cites W2923705978 @default.
- W4386864100 cites W2951670691 @default.
- W4386864100 cites W2955521507 @default.
- W4386864100 cites W2980138724 @default.
- W4386864100 cites W2982710661 @default.
- W4386864100 cites W3003356608 @default.
- W4386864100 cites W3011395023 @default.
- W4386864100 cites W3105722982 @default.
- W4386864100 cites W3205164582 @default.
- W4386864100 cites W3207288291 @default.
- W4386864100 cites W4200548340 @default.
- W4386864100 cites W4210302878 @default.
- W4386864100 cites W4213003692 @default.
- W4386864100 cites W4224254937 @default.
- W4386864100 cites W4281286294 @default.
- W4386864100 cites W4306155027 @default.
- W4386864100 cites W631297584 @default.
- W4386864100 doi "https://doi.org/10.3390/bios13090890" @default.
- W4386864100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37754124" @default.
- W4386864100 hasPublicationYear "2023" @default.
- W4386864100 type Work @default.
- W4386864100 citedByCount "0" @default.
- W4386864100 crossrefType "journal-article" @default.
- W4386864100 hasAuthorship W4386864100A5006937058 @default.
- W4386864100 hasAuthorship W4386864100A5061206817 @default.
- W4386864100 hasAuthorship W4386864100A5072012555 @default.
- W4386864100 hasAuthorship W4386864100A5092902810 @default.
- W4386864100 hasBestOaLocation W43868641001 @default.