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- W4386866125 abstract "Cordycepin is an important active metabolite of Cordyceps militaris. Xylose, an attractive feedstock for producing chemicals through microbial fermentation, cannot be effectively utilised by many reported C. militaris strains. Herein, a xylose-utilising C. militaris strain 147 produced the highest level of cordycepin (3.03 g/L) in xylose culture. Xylose, alanine, and ammonium citrate were determined as the main affecting factors on the cordycepin production using a Plackett-Burman design. The combination of these factors was optimised using response surface methodology, and the maximal 6.54 g/L of cordycepin was produced by the fungus in the optimal medium. Transcriptome analysis revealed that xylose utilisation upregulated the transcriptional levels of genes participating in purine and energy metabolisms in the fungus, which may facilitate the formation of precursors for cordycepin biosynthesis. This investigation provides new insights into the efficient production of cordycepin and is conducive to the valorisation of biomass rich in xylose." @default.
- W4386866125 created "2023-09-20" @default.
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- W4386866125 date "2023-11-01" @default.
- W4386866125 modified "2023-10-09" @default.
- W4386866125 title "High-level production of cordycepin by the xylose-utilising Cordyceps militaris strain 147 in an optimised medium" @default.
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- W4386866125 doi "https://doi.org/10.1016/j.biortech.2023.129742" @default.
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