Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386870850> ?p ?o ?g. }
- W4386870850 endingPage "4482" @default.
- W4386870850 startingPage "4466" @default.
- W4386870850 abstract "The protection and interrogation of pancreatic β-cell health and function ex vivo is a fundamental aspect of diabetes research, including mechanistic studies, evaluation of β-cell health modulators, and development and quality control of replacement β-cell populations. However, present-day islet culture formats, including traditional suspension culture as well as many recently developed microfluidic devices, suspend islets in a liquid microenvironment, disrupting mechanochemical signaling normally found in vivo and limiting β-cell viability and function in vitro. Herein, we present a novel three-dimensional (3D) microphysiological system (MPS) to extend islet health and function ex vivo by incorporating a polymerizable collagen scaffold to restore biophysical support and islet-collagen mechanobiological cues. Informed by computational models of gas and molecular transport relevant to β-cell physiology, a MPS configuration was down-selected based on simulated oxygen and nutrient delivery to collagen-encapsulated islets, and 3D-printing was applied as a readily accessible, low-cost rapid prototyping method. Recreating critical aspects of the in vivo microenvironment within the MPS via perfusion and islet-collagen interactions mitigated post-isolation ischemia and apoptosis in mouse islets over a 5-day period. In contrast, islets maintained in traditional suspension formats exhibited progressive hypoxic and apoptotic cores. Finally, dynamic glucose-stimulated insulin secretion measurements were performed on collagen-encapsulated mouse islets in the absence and presence of well-known chemical stressor thapsigargin using the MPS platform and compared to conventional protocols involving commercial perifusion machines. Overall, the MPS described here provides a user-friendly islet culture platform that not only supports long-term β-cell health and function but also enables multiparametric evaluations." @default.
- W4386870850 created "2023-09-20" @default.
- W4386870850 creator A5007764171 @default.
- W4386870850 creator A5013146110 @default.
- W4386870850 creator A5031642600 @default.
- W4386870850 creator A5063131669 @default.
- W4386870850 creator A5063953835 @default.
- W4386870850 date "2023-01-01" @default.
- W4386870850 modified "2023-10-12" @default.
- W4386870850 title "Islet-on-Chip: Promotion of Islet Health and Function via Encapsulation within a Polymerizable Fibrillar Collagen Scaffold" @default.
- W4386870850 cites W1495051530 @default.
- W4386870850 cites W1970745637 @default.
- W4386870850 cites W1981835782 @default.
- W4386870850 cites W1985112613 @default.
- W4386870850 cites W1986681896 @default.
- W4386870850 cites W1990199500 @default.
- W4386870850 cites W1995599967 @default.
- W4386870850 cites W1996887250 @default.
- W4386870850 cites W2005487978 @default.
- W4386870850 cites W2011267160 @default.
- W4386870850 cites W2017507665 @default.
- W4386870850 cites W2034316767 @default.
- W4386870850 cites W2046755374 @default.
- W4386870850 cites W2050274932 @default.
- W4386870850 cites W2052079565 @default.
- W4386870850 cites W2053079354 @default.
- W4386870850 cites W2056784230 @default.
- W4386870850 cites W2059576139 @default.
- W4386870850 cites W2060351580 @default.
- W4386870850 cites W2062059848 @default.
- W4386870850 cites W2063863657 @default.
- W4386870850 cites W2069819622 @default.
- W4386870850 cites W2070185859 @default.
- W4386870850 cites W2071571360 @default.
- W4386870850 cites W2078657091 @default.
- W4386870850 cites W2078966103 @default.
- W4386870850 cites W2088251460 @default.
- W4386870850 cites W2098764494 @default.
- W4386870850 cites W2124025841 @default.
- W4386870850 cites W2131873563 @default.
- W4386870850 cites W2137034111 @default.
- W4386870850 cites W2149036744 @default.
- W4386870850 cites W2156016113 @default.
- W4386870850 cites W2183793823 @default.
- W4386870850 cites W2317184785 @default.
- W4386870850 cites W2342593170 @default.
- W4386870850 cites W2343128608 @default.
- W4386870850 cites W2508473328 @default.
- W4386870850 cites W2581348935 @default.
- W4386870850 cites W2618304073 @default.
- W4386870850 cites W2753001672 @default.
- W4386870850 cites W2765323542 @default.
- W4386870850 cites W2780573361 @default.
- W4386870850 cites W2794374249 @default.
- W4386870850 cites W2807893746 @default.
- W4386870850 cites W2810849157 @default.
- W4386870850 cites W2885547437 @default.
- W4386870850 cites W2890799880 @default.
- W4386870850 cites W2896278481 @default.
- W4386870850 cites W2920313553 @default.
- W4386870850 cites W2955953853 @default.
- W4386870850 cites W2971191203 @default.
- W4386870850 cites W2977478555 @default.
- W4386870850 cites W2984122963 @default.
- W4386870850 cites W2989645149 @default.
- W4386870850 cites W3003240648 @default.
- W4386870850 cites W3012718103 @default.
- W4386870850 cites W3024506005 @default.
- W4386870850 cites W3048463482 @default.
- W4386870850 cites W3120754950 @default.
- W4386870850 cites W3122062588 @default.
- W4386870850 cites W3123410441 @default.
- W4386870850 cites W3128226077 @default.
- W4386870850 cites W3135343328 @default.
- W4386870850 cites W3137627959 @default.
- W4386870850 cites W3147326649 @default.
- W4386870850 cites W3158173674 @default.
- W4386870850 cites W4292755723 @default.
- W4386870850 cites W4310592640 @default.
- W4386870850 doi "https://doi.org/10.1039/d3lc00371j" @default.
- W4386870850 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37740372" @default.
- W4386870850 hasPublicationYear "2023" @default.
- W4386870850 type Work @default.
- W4386870850 citedByCount "0" @default.
- W4386870850 crossrefType "journal-article" @default.
- W4386870850 hasAuthorship W4386870850A5007764171 @default.
- W4386870850 hasAuthorship W4386870850A5013146110 @default.
- W4386870850 hasAuthorship W4386870850A5031642600 @default.
- W4386870850 hasAuthorship W4386870850A5063131669 @default.
- W4386870850 hasAuthorship W4386870850A5063953835 @default.
- W4386870850 hasConcept C134018914 @default.
- W4386870850 hasConcept C136229726 @default.
- W4386870850 hasConcept C150903083 @default.
- W4386870850 hasConcept C165220095 @default.
- W4386870850 hasConcept C185592680 @default.
- W4386870850 hasConcept C207001950 @default.
- W4386870850 hasConcept C26291073 @default.
- W4386870850 hasConcept C2779306644 @default.