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- W4386879220 abstract "The Ca2+ release in microdomains formed by intercompartmental contacts, such as mitochondria-associated endoplasmic reticulum membranes (MAMs), encodes a signal that contributes to Ca2+ homeostasis and cell fate control. However, the composition and function of MAMs remain to be fully defined. Here, we focused on the transient receptor potential vanilloid 1 (TRPV1), a Ca2+-permeable ion channel and a polymodal nociceptor. We found TRPV1 channels in the reticular membrane, including some at MAMs, in a rat cardiomyoblast cell line (SV40-transformed H9c2) by Western blotting, immunostaining, cell fractionation, and proximity ligation assay. We used chemical and genetic probes to perform Ca2+ imaging in four cellular compartments: the endoplasmic reticulum (ER), cytoplasm, mitochondrial matrix, and mitochondrial surface. Our results showed that the ER Ca2+ released through TRPV1 channels is detected at the mitochondrial outer membrane and transferred to the mitochondria. Finally, we observed that prolonged TRPV1 modulation for 30 min alters the intracellular Ca2+ equilibrium and influences the MAM structure or the hypoxia/reoxygenation-induced cell death. Thus, our study provides the first evidence that TRPV1 channels contribute to MAM Ca2+ exchanges." @default.
- W4386879220 created "2023-09-21" @default.
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- W4386879220 date "2023-09-20" @default.
- W4386879220 modified "2023-10-18" @default.
- W4386879220 title "TRPV1 Channels Are New Players in the Reticulum–Mitochondria Ca2+ Coupling in a Rat Cardiomyoblast Cell Line" @default.
- W4386879220 cites W104189985 @default.
- W4386879220 cites W1511198581 @default.
- W4386879220 cites W1582245657 @default.
- W4386879220 cites W1822580098 @default.
- W4386879220 cites W1928784746 @default.
- W4386879220 cites W1968320713 @default.
- W4386879220 cites W1968365147 @default.
- W4386879220 cites W1969233157 @default.
- W4386879220 cites W1975987057 @default.
- W4386879220 cites W1980959257 @default.
- W4386879220 cites W1981659462 @default.
- W4386879220 cites W1988798680 @default.
- W4386879220 cites W1996146434 @default.
- W4386879220 cites W1997511216 @default.
- W4386879220 cites W2003396297 @default.
- W4386879220 cites W2009667219 @default.
- W4386879220 cites W2015426350 @default.
- W4386879220 cites W2015536770 @default.
- W4386879220 cites W2020352730 @default.
- W4386879220 cites W2027966934 @default.
- W4386879220 cites W2038221909 @default.
- W4386879220 cites W2042646075 @default.
- W4386879220 cites W204998232 @default.
- W4386879220 cites W2050781058 @default.
- W4386879220 cites W2056034142 @default.
- W4386879220 cites W2056775926 @default.
- W4386879220 cites W2061627146 @default.
- W4386879220 cites W2065453809 @default.
- W4386879220 cites W2070148593 @default.
- W4386879220 cites W2077590811 @default.
- W4386879220 cites W2089781399 @default.
- W4386879220 cites W2094657005 @default.
- W4386879220 cites W2094999489 @default.
- W4386879220 cites W2096579721 @default.
- W4386879220 cites W2099902423 @default.
- W4386879220 cites W2104659034 @default.
- W4386879220 cites W2105963832 @default.
- W4386879220 cites W2106201634 @default.
- W4386879220 cites W2107705149 @default.
- W4386879220 cites W2111617797 @default.
- W4386879220 cites W2122778205 @default.
- W4386879220 cites W2125479650 @default.
- W4386879220 cites W2128489958 @default.
- W4386879220 cites W2136177283 @default.
- W4386879220 cites W2140388091 @default.
- W4386879220 cites W2148185042 @default.
- W4386879220 cites W2152757414 @default.
- W4386879220 cites W2153535707 @default.
- W4386879220 cites W2158551386 @default.
- W4386879220 cites W2223869592 @default.
- W4386879220 cites W2276380881 @default.
- W4386879220 cites W2281735941 @default.
- W4386879220 cites W2469871926 @default.
- W4386879220 cites W2472925387 @default.
- W4386879220 cites W2524439359 @default.
- W4386879220 cites W2534987268 @default.
- W4386879220 cites W2598521796 @default.
- W4386879220 cites W2599204814 @default.
- W4386879220 cites W2610619839 @default.
- W4386879220 cites W2611851783 @default.
- W4386879220 cites W2613365825 @default.
- W4386879220 cites W2618042792 @default.
- W4386879220 cites W2620236757 @default.
- W4386879220 cites W2624937154 @default.
- W4386879220 cites W2651812890 @default.
- W4386879220 cites W2749621854 @default.
- W4386879220 cites W2766966618 @default.
- W4386879220 cites W2783880365 @default.
- W4386879220 cites W2788640168 @default.
- W4386879220 cites W2800335290 @default.
- W4386879220 cites W2802543878 @default.
- W4386879220 cites W2806263581 @default.
- W4386879220 cites W2809062142 @default.
- W4386879220 cites W2900649129 @default.
- W4386879220 cites W2921717581 @default.
- W4386879220 cites W2945471566 @default.
- W4386879220 cites W2953602755 @default.
- W4386879220 cites W2969731445 @default.
- W4386879220 cites W2971274207 @default.
- W4386879220 cites W3000062473 @default.
- W4386879220 cites W3005884339 @default.
- W4386879220 cites W3026360123 @default.
- W4386879220 cites W3027245524 @default.