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- W4386889285 abstract "We present a detailed protocol for sequencing full-length mRNA isoforms using the Oxford nanopore long-read sequencing technology. We describe steps for poly(A) RNA isolation, library preparation, and cDNA size selection. We then detail procedures for sequencing and processing and a computational framework to identify exon couplings and assign mRNA 5′ ends and 3′ ends to each other. Our approach enables the identification of links between transcription initiation and co-transcriptional RNA processing events. For complete details on the use and execution of this protocol, please refer to Alfonso-Gonzalez et al.1" @default.
- W4386889285 created "2023-09-21" @default.
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- W4386889285 date "2023-12-01" @default.
- W4386889285 modified "2023-10-11" @default.
- W4386889285 title "Identification of regulatory links between transcription and RNA processing with long-read sequencing" @default.
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- W4386889285 doi "https://doi.org/10.1016/j.xpro.2023.102505" @default.
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