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• W4386910410 abstract "A novel immunochromatography strip (ICS) based on the DNA-bridged double gold nanoparticles was established and evaluated for the dual-mode detection of ochratoxin A (OTA). For this purpose, the streptavidin was coupled with the big size of gold nanoparticle (40 nm, AuNP), the OTA monoclonal antibody and biotinylated DNA-SH were simultaneously immobilized on the small size of AuNP (20 nm), and then the enhanced ICS was self-assembled and evaluated. The dual-mode detection of semi-quantification and quantification had been achieved and performed by the proposed ICS. The LOD for semi-quantification (semi-Q-LOD) was 0.06 ng/mL by the directly naked eye (eightfold enhanced than conventional ICS). By the on-site reader, the LOD for quantification (Q-LOD) was 0.03 ng/mL (threefold enhanced), with the detection range between 0.03 and 1.2 ng/mL. The specificity, reliability, and practicability had been well represented. Furthermore, the OTA-positive results of the enhanced ICS method correlated well with those obtained by the referenced HPLC-MS/MS for the market samples. This study provided a new ICS pattern of semi-quantification and quantification for OTA contamination, which could be used as a valuable reference for improving the ICS technology and enhancing the sensitivity." @default.
• W4386910410 created "2023-09-22" @default.
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• W4386910410 date "2023-09-21" @default.
• W4386910410 modified "2023-10-18" @default.
• W4386910410 title "DNA‐bridged double gold nanoparticles‐based immunochromatography for dual‐mode detection of ochratoxin A" @default.
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• W4386910410 doi "https://doi.org/10.1111/1750-3841.16763" @default.
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