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- W4386916181 abstract "Abstract The Cryptococcus genus comprises more than 100 species, of which C. neoformans and C. gattii are the leading cause of cryptococcosis. The distribution of C. gattii and C. neoformans species complexes has been extensively studied and widely reported globally. Other species such as Naganishia albida , Papiliotrema laurentii , and Papiliotrema flavescens have been reported as pathogenic yeasts. Since there are no reports of environmental isolation in the Boyacá region (Colombia), this study aimed to isolate and characterize Cryptococcus and Cryptococcus -like yeasts from pigeon feces, Eucalyptus , and olive trees distributed in the municipalities of Tunja and Ricaute Alto. The environmental data was recovered, and the isolations obtained were identified by microscopy, biochemical test, MALDI-TOF MS, URA5 -RFLP, and sequencing of the ITS and LSU loci. For the 93 pigeon dropping samples collected in Tunja, 23 yielded to C. neoformans , 3 to N. globosa , 2 N. albida and 1 to P. laurentii . Of the 1188 samples collected from olive trees, 17 (1.43%) positive samples were identified as C. gattii species complex (4), C. neoformans species complex (2), P. laurentii (3), N. albida (2), N. globosa (5) and P. flavescens (1). Likewise, specimens of C. neoformans presented molecular type VNI and molecular type VNII; for C. gattii the molecular types found were VGIII and one VGIV by URA5 -RFLP but VGIII by MALDI-TOF and sequencing of the ITS and LSU. Therefore, it can be concluded that the species of Cryptococcus, Naganishia and Papiliotrema genera, are present in the environment of Boyacá, and show a predilection for climate conditions that are typical of this region." @default.
- W4386916181 created "2023-09-22" @default.
- W4386916181 creator A5036384543 @default.
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- W4386916181 creator A5058443876 @default.
- W4386916181 creator A5064462178 @default.
- W4386916181 creator A5067012004 @default.
- W4386916181 date "2023-09-21" @default.
- W4386916181 modified "2023-10-16" @default.
- W4386916181 title "First report of environmental isolation of Cryptococcus and Cryptococcus-like yeasts from Boyacá, Colombia" @default.
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