Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386953172> ?p ?o ?g. }
- W4386953172 abstract "Abstract Background Uncontrolled proliferation of pulmonary artery smooth muscle cells (PASMCs) contributes to the pathogenesis of pulmonary arterial hypertension (PAH). In this work, we defined the precise part of circ_0068481 in PASMC proliferation and migration induced by hypoxia. We hypothesized that circ_0068481 enhanced hypoxia-induced PASMC proliferation, invasion, and migration through the microRNA (miR)-361-3p/Krüppel-like factor 5 (KLF5) pathway. Methods Human PASMCs (hPASMCs) were exposed to hypoxic (3% O2) conditions. Circ_0068481, miR-361-3p, and KLF5 levels were gauged by qRT-PCR and western blot. Cell viability, proliferation, invasion, and migration were detected by XTT, EdU incorporation, transwell, and wound-healing assays, respectively. Dual-luciferase reporter, RNA immunoprecipitation, and RNA pull-down assays were performed to confirm the direct relationship between miR-361-3p and circ_0068481 or KLF5. Results Circ_0068481 expression was increased in the serum of PAH patients and hypoxia-induced hPASMCs. Downregulation of circ_0068481 attenuated hypoxia-induced promotion in hPASMC proliferation, invasion, and migration. Circ_0068481 directly targeted miR-361-3p, and miR-361-3p downregulation reversed the inhibitory effects of circ_0068481 silencing on hypoxia-induced hPASMC proliferation, invasion, and migration. KLF5 was a direct miR-361-3p target, and miR-361-3p upregulation mitigated hypoxia-induced hPASMC proliferation, invasion, and migration by inhibiting KLF5 expression. Moreover, circ_0068481-induced KLF5 expression by binding to miR-361-3p in hypoxic hPASMCs. Conclusions Circ_0068481 knockdown ameliorated hypoxia-induced hPASMC proliferation, invasion, and migration at least in part through the miR-361-3p/KLF5 axis." @default.
- W4386953172 created "2023-09-23" @default.
- W4386953172 creator A5019005078 @default.
- W4386953172 creator A5020135358 @default.
- W4386953172 creator A5025013056 @default.
- W4386953172 creator A5042804558 @default.
- W4386953172 creator A5072947490 @default.
- W4386953172 creator A5074575547 @default.
- W4386953172 creator A5074854917 @default.
- W4386953172 date "2023-09-20" @default.
- W4386953172 modified "2023-10-12" @default.
- W4386953172 title "Circ_0068481 affects the human pulmonary artery smooth muscle cells’ progression by miR-361-3p/KLF5 axis" @default.
- W4386953172 cites W1892182006 @default.
- W4386953172 cites W1964727088 @default.
- W4386953172 cites W2117977572 @default.
- W4386953172 cites W2130687298 @default.
- W4386953172 cites W2210577735 @default.
- W4386953172 cites W2291993944 @default.
- W4386953172 cites W2319836054 @default.
- W4386953172 cites W2734176231 @default.
- W4386953172 cites W2769302225 @default.
- W4386953172 cites W2781436969 @default.
- W4386953172 cites W2938489589 @default.
- W4386953172 cites W2954167484 @default.
- W4386953172 cites W2967043226 @default.
- W4386953172 cites W2968663009 @default.
- W4386953172 cites W2977371882 @default.
- W4386953172 cites W2982673301 @default.
- W4386953172 cites W2990113818 @default.
- W4386953172 cites W2997142879 @default.
- W4386953172 cites W3005239251 @default.
- W4386953172 cites W3007621409 @default.
- W4386953172 cites W3016169192 @default.
- W4386953172 cites W3036212082 @default.
- W4386953172 cites W3040360065 @default.
- W4386953172 cites W3041511201 @default.
- W4386953172 cites W3081161470 @default.
- W4386953172 cites W3083774861 @default.
- W4386953172 cites W3085173548 @default.
- W4386953172 cites W3088816888 @default.
- W4386953172 cites W3090396682 @default.
- W4386953172 cites W3092263480 @default.
- W4386953172 cites W3094531751 @default.
- W4386953172 cites W3110409082 @default.
- W4386953172 cites W3133707758 @default.
- W4386953172 cites W3150453735 @default.
- W4386953172 cites W3198444924 @default.
- W4386953172 cites W3200061637 @default.
- W4386953172 cites W3215445868 @default.
- W4386953172 cites W4281610087 @default.
- W4386953172 doi "https://doi.org/10.1093/ajh/hpad028" @default.
- W4386953172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37738301" @default.
- W4386953172 hasPublicationYear "2023" @default.
- W4386953172 type Work @default.
- W4386953172 citedByCount "0" @default.
- W4386953172 crossrefType "journal-article" @default.
- W4386953172 hasAuthorship W4386953172A5019005078 @default.
- W4386953172 hasAuthorship W4386953172A5020135358 @default.
- W4386953172 hasAuthorship W4386953172A5025013056 @default.
- W4386953172 hasAuthorship W4386953172A5042804558 @default.
- W4386953172 hasAuthorship W4386953172A5072947490 @default.
- W4386953172 hasAuthorship W4386953172A5074575547 @default.
- W4386953172 hasAuthorship W4386953172A5074854917 @default.
- W4386953172 hasConcept C104317684 @default.
- W4386953172 hasConcept C119056186 @default.
- W4386953172 hasConcept C126322002 @default.
- W4386953172 hasConcept C127561419 @default.
- W4386953172 hasConcept C137738243 @default.
- W4386953172 hasConcept C145059251 @default.
- W4386953172 hasConcept C1491633281 @default.
- W4386953172 hasConcept C173396325 @default.
- W4386953172 hasConcept C178790620 @default.
- W4386953172 hasConcept C185592680 @default.
- W4386953172 hasConcept C2776415932 @default.
- W4386953172 hasConcept C2780940725 @default.
- W4386953172 hasConcept C2780942790 @default.
- W4386953172 hasConcept C502942594 @default.
- W4386953172 hasConcept C540031477 @default.
- W4386953172 hasConcept C54355233 @default.
- W4386953172 hasConcept C55493867 @default.
- W4386953172 hasConcept C62112901 @default.
- W4386953172 hasConcept C71924100 @default.
- W4386953172 hasConcept C7836513 @default.
- W4386953172 hasConcept C81885089 @default.
- W4386953172 hasConcept C86803240 @default.
- W4386953172 hasConcept C95444343 @default.
- W4386953172 hasConceptScore W4386953172C104317684 @default.
- W4386953172 hasConceptScore W4386953172C119056186 @default.
- W4386953172 hasConceptScore W4386953172C126322002 @default.
- W4386953172 hasConceptScore W4386953172C127561419 @default.
- W4386953172 hasConceptScore W4386953172C137738243 @default.
- W4386953172 hasConceptScore W4386953172C145059251 @default.
- W4386953172 hasConceptScore W4386953172C1491633281 @default.
- W4386953172 hasConceptScore W4386953172C173396325 @default.
- W4386953172 hasConceptScore W4386953172C178790620 @default.
- W4386953172 hasConceptScore W4386953172C185592680 @default.
- W4386953172 hasConceptScore W4386953172C2776415932 @default.
- W4386953172 hasConceptScore W4386953172C2780940725 @default.
- W4386953172 hasConceptScore W4386953172C2780942790 @default.
- W4386953172 hasConceptScore W4386953172C502942594 @default.