Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386979887> ?p ?o ?g. }
- W4386979887 abstract "Cysteine dioxygenase 1 (CDO1) is frequently methylated, and its expression is decreased in many human cancers including breast cancer (BC). However, the functional and mechanistic aspects of CDO1 inactivation in BC are poorly understood, and the diagnostic significance of serum CDO1 methylation remains unclear.We performed bioinformatics analysis of publicly available databases and employed MassARRAY EpiTYPER methylation sequencing technology to identify differentially methylated sites in the CDO1 promoter of BC tissues compared to normal adjacent tissues (NATs). Subsequently, we developed a MethyLight assay using specific primers and probes for these CpG sites to detect the percentage of methylated reference (PMR) of the CDO1 promoter. Furthermore, both LentiCRISPR/dCas9-Tet1CD-based CDO1-targeted demethylation system and CDO1 overexpression strategy were utilized to detect the function and underlying mechanism of CDO1 in BC. Finally, the early diagnostic value of CDO1 as a methylation biomarker in BC serum was evaluated.CDO1 promoter was hypermethylated in BC tissues, which was related to poor prognosis (p < .05). The CRISPR/dCas9-based targeted demethylation system significantly reduced the PMR of CDO1 promotor and increased CDO1 expression in BC cells. Consequently, this leads to suppression of cell proliferation, migration and invasion. Additionally, we found that CDO1 exerted a tumour suppressor effect by inhibiting the cell cycle, promoting cell apoptosis and ferroptosis. Furthermore, we employed the MethyLight to detect CDO1 PMR in BC serum, and we discovered that serum CDO1 methylation was an effective non-invasive biomarker for early diagnosis of BC.CDO1 is hypermethylated and acts as a tumour suppressor gene in BC. Epigenetic editing of abnormal CDO1 methylation could have a crucial role in the clinical treatment and prognosis of BC. Additionally, serum CDO1 methylation holds promise as a valuable biomarker for the early diagnosis and management of BC." @default.
- W4386979887 created "2023-09-24" @default.
- W4386979887 creator A5000894606 @default.
- W4386979887 creator A5000940922 @default.
- W4386979887 creator A5001864411 @default.
- W4386979887 creator A5006619535 @default.
- W4386979887 creator A5006827480 @default.
- W4386979887 creator A5010123110 @default.
- W4386979887 creator A5016790981 @default.
- W4386979887 creator A5018289057 @default.
- W4386979887 creator A5018742393 @default.
- W4386979887 creator A5021108291 @default.
- W4386979887 creator A5022105543 @default.
- W4386979887 creator A5023760424 @default.
- W4386979887 creator A5025032602 @default.
- W4386979887 creator A5031598645 @default.
- W4386979887 creator A5043610472 @default.
- W4386979887 creator A5060470068 @default.
- W4386979887 creator A5073845618 @default.
- W4386979887 creator A5079791147 @default.
- W4386979887 creator A5086859444 @default.
- W4386979887 creator A5091413207 @default.
- W4386979887 date "2023-09-01" @default.
- W4386979887 modified "2023-10-16" @default.
- W4386979887 title "Targeted demethylation of the CDO1 promoter based on CRISPR system inhibits the malignant potential of breast cancer cells" @default.
- W4386979887 cites W1583605880 @default.
- W4386979887 cites W1927355923 @default.
- W4386979887 cites W1965301144 @default.
- W4386979887 cites W1968328969 @default.
- W4386979887 cites W1977709885 @default.
- W4386979887 cites W1981463058 @default.
- W4386979887 cites W1982935532 @default.
- W4386979887 cites W2000434123 @default.
- W4386979887 cites W2006852463 @default.
- W4386979887 cites W2017394727 @default.
- W4386979887 cites W2029484950 @default.
- W4386979887 cites W2029816080 @default.
- W4386979887 cites W2032882684 @default.
- W4386979887 cites W2042464369 @default.
- W4386979887 cites W2055446766 @default.
- W4386979887 cites W2064393241 @default.
- W4386979887 cites W2076932702 @default.
- W4386979887 cites W2082857671 @default.
- W4386979887 cites W2093836594 @default.
- W4386979887 cites W2101108029 @default.
- W4386979887 cites W2106680700 @default.
- W4386979887 cites W2108664872 @default.
- W4386979887 cites W2112060104 @default.
- W4386979887 cites W2121090755 @default.
- W4386979887 cites W2139122477 @default.
- W4386979887 cites W2146645283 @default.
- W4386979887 cites W2148760132 @default.
- W4386979887 cites W2157473097 @default.
- W4386979887 cites W2163267129 @default.
- W4386979887 cites W2163311832 @default.
- W4386979887 cites W2314493329 @default.
- W4386979887 cites W2346829986 @default.
- W4386979887 cites W2414449891 @default.
- W4386979887 cites W2414836841 @default.
- W4386979887 cites W2525328967 @default.
- W4386979887 cites W2525877111 @default.
- W4386979887 cites W2740317277 @default.
- W4386979887 cites W2750926847 @default.
- W4386979887 cites W2762509232 @default.
- W4386979887 cites W2769191782 @default.
- W4386979887 cites W2770434260 @default.
- W4386979887 cites W2774170088 @default.
- W4386979887 cites W2797336915 @default.
- W4386979887 cites W2801793270 @default.
- W4386979887 cites W2886080030 @default.
- W4386979887 cites W2890668604 @default.
- W4386979887 cites W2907084168 @default.
- W4386979887 cites W2937354235 @default.
- W4386979887 cites W2946994270 @default.
- W4386979887 cites W2973580032 @default.
- W4386979887 cites W2994655911 @default.
- W4386979887 cites W3005685791 @default.
- W4386979887 cites W3048653545 @default.
- W4386979887 cites W3123940477 @default.
- W4386979887 cites W3137719665 @default.
- W4386979887 cites W3139370705 @default.
- W4386979887 cites W3147142721 @default.
- W4386979887 cites W3169544586 @default.
- W4386979887 cites W3169699960 @default.
- W4386979887 cites W3178499714 @default.
- W4386979887 cites W3211524417 @default.
- W4386979887 cites W3212246792 @default.
- W4386979887 cites W4205783315 @default.
- W4386979887 cites W4206501634 @default.
- W4386979887 cites W4210426211 @default.
- W4386979887 cites W4214915560 @default.
- W4386979887 cites W4220716665 @default.
- W4386979887 cites W4224253316 @default.
- W4386979887 cites W4283077974 @default.
- W4386979887 cites W4285093742 @default.
- W4386979887 cites W4294579002 @default.
- W4386979887 cites W4311835442 @default.
- W4386979887 doi "https://doi.org/10.1002/ctm2.1423" @default.
- W4386979887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37740473" @default.
- W4386979887 hasPublicationYear "2023" @default.