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• W4386986585 abstract "Tissue autofluorescence poses significant challenges for RNA and protein analysis using fluorescence-based techniques. Here, we present a protocol that combines oxidation-mediated autofluorescence reduction with detergent-based tissue permeabilization for whole-mount RNA-fluorescence in situ hybridization (FISH) on mouse embryonic limb buds. We describe the steps for embryo collection, fixation, photochemical bleaching, permeabilization, and RNA-FISH, followed by optical clearing of RNA-FISH and immunofluorescence samples for imaging. The protocol alleviates the need for digital image post-processing to remove autofluorescence and is applicable to other tissues, organs, and vertebrate embryos." @default.
• W4386986585 created "2023-09-24" @default.
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• W4386986585 date "2023-12-01" @default.
• W4386986585 modified "2023-10-17" @default.
• W4386986585 title "Optimized protocol for whole-mount RNA fluorescent in situ hybridization using oxidation-mediated autofluorescence reduction on mouse embryos" @default.
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• W4386986585 doi "https://doi.org/10.1016/j.xpro.2023.102603" @default.
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