Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386989184> ?p ?o ?g. }
- W4386989184 endingPage "117223" @default.
- W4386989184 startingPage "117223" @default.
- W4386989184 abstract "Eugenia uniflora Linn, popularly known as ‘pitanga’, is a native plant endemic to Brazil that belongs to the Myrtaceae family. Its traditional use (leaves infusion) has been reported for the treatment of different diseases, including hypertension, inflammation, and as a diuretic agent. Considering the snakebite problem and the rich molecule repertoire of this herbal species, studies that evaluate its antiophidic potential are relevant for a broad social impact. This approach aims to evaluate the anti-inflammatory and antiophidic potential in vitro and in vivo of the extract (aqueous) and a fraction (ethyl acetate) of E. uniflora leaves against Bothrops leucurus and Bothrops brazili venoms. Extract and fraction from E. uniflora leaves were obtained by turbo-extraction and partitioning. The cytotoxicity was assayed on normal cell lines (Vero E6 and 3T3) using the 3-methyl-[4-5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide method. The anti-inflammatory activity of the aqueous extract was analyzed in vivo in the zymosan-induced air pouch model, and the leukocytes migration and other molecular inflammatory mediators quantified (myeloperoxidase, total protein, pro-inflammatory cytokine, malondialdehyde, and glutathione). In vitro, the antiophidic effect was evaluated by the ability of the E. uniflora extract and fraction to inhibit the enzymatic action (proteolytic, phospholipase A2, and hyaluronidase) of B. leucurus and B. brazili venoms. In addition, the antiophidic action in vivo was investigated after treatment with E. uniflora extract and fraction (50, 100, and 200 mg/kg) in the B. leucurus venom-induced paw edema with an evaluation of the antiedematogenic effect and quantification of myeloperoxidase (MPO) and pro-inflammatory cytokine levels. The E. uniflora leaves extract (7.8–125 mg/mL) revealed no toxicity in cell culture, but reduced MTT by 47% at the highest concentration (250 mg/mL) in Vero E6 cells. In contrast, the E. uniflora fraction (7.8–250 mg/mL) showed no cytotoxicity for both cell lines. In the air pouch model, E. uniflora leaves extract demonstrated anti-inflammatory activity, reducing cell migration, MPO activity, protein, malondialdehyde, and proinflammatory cytokines, and increased glutathione levels. Evaluating the antiophidic action in vitro, E. uniflora extract and fraction inhibited the proteolytic, phospholipase, and hyaluronidase effects of B. leucurus and B. brazili venoms at low concentrations. In addition, the extract and fraction also demonstrated in vivo antiophidic activity by reducing edema in the first 0.5 h after treatment, besides reducing MPO and pro-inflammatory cytokines levels. E. uniflora leaves extract showed cytotoxicity only at the highest concentration while the fraction revealed no toxic effect in vitro. This approach showed for the first time that the aqueous extract and ethyl acetate fraction of E. uniflora leaves has similar antiophidic action in vitro and in vivo, with antiedematogenic and anti-inflammatory effects and the ability to inhibit the enzymatic action of B. leucurus and B. brazili venoms. Therefore, this study points to the presence of bioactive components in the leaves of E. uniflora useful for the treatment of inflammatory disorders and ophidian accidents, expanding the therapeutic potential of this herbal species." @default.
- W4386989184 created "2023-09-24" @default.
- W4386989184 creator A5006466399 @default.
- W4386989184 creator A5010736475 @default.
- W4386989184 creator A5041447433 @default.
- W4386989184 creator A5044359321 @default.
- W4386989184 creator A5048003612 @default.
- W4386989184 creator A5048661242 @default.
- W4386989184 creator A5057239850 @default.
- W4386989184 creator A5065271407 @default.
- W4386989184 creator A5089058079 @default.
- W4386989184 date "2024-01-01" @default.
- W4386989184 modified "2023-10-17" @default.
- W4386989184 title "In vitro and in vivo anti-inflammatory and antiophidic effects of the extract and fraction of Eugenia uniflora" @default.
- W4386989184 cites W1970652854 @default.
- W4386989184 cites W1970927146 @default.
- W4386989184 cites W1988777181 @default.
- W4386989184 cites W1993943341 @default.
- W4386989184 cites W2020358486 @default.
- W4386989184 cites W2023637921 @default.
- W4386989184 cites W2028031080 @default.
- W4386989184 cites W2055015556 @default.
- W4386989184 cites W2058398296 @default.
- W4386989184 cites W2059885690 @default.
- W4386989184 cites W2061565150 @default.
- W4386989184 cites W2068840229 @default.
- W4386989184 cites W2072564805 @default.
- W4386989184 cites W2073136536 @default.
- W4386989184 cites W2083269140 @default.
- W4386989184 cites W2114918609 @default.
- W4386989184 cites W2123105952 @default.
- W4386989184 cites W2256742438 @default.
- W4386989184 cites W2521574223 @default.
- W4386989184 cites W2567569673 @default.
- W4386989184 cites W2743704406 @default.
- W4386989184 cites W2766212784 @default.
- W4386989184 cites W2771472591 @default.
- W4386989184 cites W2797648265 @default.
- W4386989184 cites W2803019100 @default.
- W4386989184 cites W2899460209 @default.
- W4386989184 cites W2902283202 @default.
- W4386989184 cites W2921381937 @default.
- W4386989184 cites W2946490258 @default.
- W4386989184 cites W2965443052 @default.
- W4386989184 cites W2996706808 @default.
- W4386989184 cites W3011366455 @default.
- W4386989184 cites W3024738743 @default.
- W4386989184 cites W3049128907 @default.
- W4386989184 cites W3049297743 @default.
- W4386989184 cites W3081085071 @default.
- W4386989184 cites W3106125810 @default.
- W4386989184 cites W3204090440 @default.
- W4386989184 cites W4200430151 @default.
- W4386989184 cites W4212926360 @default.
- W4386989184 cites W4214893611 @default.
- W4386989184 cites W4229446325 @default.
- W4386989184 cites W4293247451 @default.
- W4386989184 cites W4294390563 @default.
- W4386989184 cites W4306158160 @default.
- W4386989184 cites W4313424571 @default.
- W4386989184 cites W4362218836 @default.
- W4386989184 cites W4382401192 @default.
- W4386989184 cites W4384525972 @default.
- W4386989184 doi "https://doi.org/10.1016/j.jep.2023.117223" @default.
- W4386989184 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37748636" @default.
- W4386989184 hasPublicationYear "2024" @default.
- W4386989184 type Work @default.
- W4386989184 citedByCount "0" @default.
- W4386989184 crossrefType "journal-article" @default.
- W4386989184 hasAuthorship W4386989184A5006466399 @default.
- W4386989184 hasAuthorship W4386989184A5010736475 @default.
- W4386989184 hasAuthorship W4386989184A5041447433 @default.
- W4386989184 hasAuthorship W4386989184A5044359321 @default.
- W4386989184 hasAuthorship W4386989184A5048003612 @default.
- W4386989184 hasAuthorship W4386989184A5048661242 @default.
- W4386989184 hasAuthorship W4386989184A5057239850 @default.
- W4386989184 hasAuthorship W4386989184A5065271407 @default.
- W4386989184 hasAuthorship W4386989184A5089058079 @default.
- W4386989184 hasConcept C150903083 @default.
- W4386989184 hasConcept C181199279 @default.
- W4386989184 hasConcept C185592680 @default.
- W4386989184 hasConcept C207001950 @default.
- W4386989184 hasConcept C2776725428 @default.
- W4386989184 hasConcept C2779448229 @default.
- W4386989184 hasConcept C2779703632 @default.
- W4386989184 hasConcept C2779815518 @default.
- W4386989184 hasConcept C2780375986 @default.
- W4386989184 hasConcept C55493867 @default.
- W4386989184 hasConcept C556039675 @default.
- W4386989184 hasConcept C71924100 @default.
- W4386989184 hasConcept C86803240 @default.
- W4386989184 hasConcept C98274493 @default.
- W4386989184 hasConceptScore W4386989184C150903083 @default.
- W4386989184 hasConceptScore W4386989184C181199279 @default.
- W4386989184 hasConceptScore W4386989184C185592680 @default.
- W4386989184 hasConceptScore W4386989184C207001950 @default.
- W4386989184 hasConceptScore W4386989184C2776725428 @default.
- W4386989184 hasConceptScore W4386989184C2779448229 @default.