Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387014993> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W4387014993 endingPage "AB37" @default.
- W4387014993 startingPage "AB37" @default.
- W4387014993 abstract "Bullous systemic lupus erythematosus (BSLE) and epidermolysis bullosa acquisita (EBA) are immunobullous disorders caused by antibodies against type-VII collagen. Histologic presentations are identical, with subepidermal blisters and neutrophilic infiltrates in the dermis. Direct immunofluorescent staining (DIF) shows linear depositions of IgG and C3 and uncommonly IgM/IgA along the dermo- epidermal junction (DEJ). Salt-split skin shows dermal deposition of immunoglobulins. Clinically, they present with erythematous dermal plaques, and tense vesicles/bullae involving the face, trunk, and extensor extremities. Clinico-histopathologic differentiation may be difficult as 30-50% of BSLE precede SLE and EBA can present with systemic symptoms of fever, non-erosive arthritis and be associated with SLE. Western-blot of serum IgG subclasses have shown elevated IgG2/IgG3 and IgG1/IgG4 in BSLE and EBA, respectively. DIF of IgG subclasses in perilesional skin may aid in the differentiation between BSLE and EBA. We report 4 patients with clinico-histopathologic differential diagnoses of BSLE or EBA. Frozen tissue-slides from perilesional skin was obtained from each patient. Primary rabbit anti-human monoclonal antibodies against IgG1/IgG2/IgG3/IgG4 were added to the tissue and then incubated with goat anti-rabbit antibodies conjugated with Alexa-Fluor-488. Images were captured via Olympus microscope 10x and 20x magnification. Two people blinded to the study evaluated the intensity of the DIF staining. At the DEJ, DIF showed stronger linear IgG2 and IgG3 staining in BSLE, whereas EBA showed stronger linear IgG1 and IgG4 staining. In cases with features consistent with both BSLE and EBA, IgG subclass analysis via DIF, can be useful to prioritize diagnostic options and to decide therapeutic management." @default.
- W4387014993 created "2023-09-26" @default.
- W4387014993 creator A5020385507 @default.
- W4387014993 creator A5046835137 @default.
- W4387014993 creator A5052194232 @default.
- W4387014993 creator A5083780142 @default.
- W4387014993 date "2023-09-01" @default.
- W4387014993 modified "2023-09-26" @default.
- W4387014993 title "44274 Direct immunofluorescence IgG autoantibody subclass analysis as a tool to differentiate between bullous systemic lupus erythematosus and epidermolysis bullosa acquisita" @default.
- W4387014993 doi "https://doi.org/10.1016/j.jaad.2023.07.151" @default.
- W4387014993 hasPublicationYear "2023" @default.
- W4387014993 type Work @default.
- W4387014993 citedByCount "0" @default.
- W4387014993 crossrefType "journal-article" @default.
- W4387014993 hasAuthorship W4387014993A5020385507 @default.
- W4387014993 hasAuthorship W4387014993A5046835137 @default.
- W4387014993 hasAuthorship W4387014993A5052194232 @default.
- W4387014993 hasAuthorship W4387014993A5083780142 @default.
- W4387014993 hasConcept C142724271 @default.
- W4387014993 hasConcept C159654299 @default.
- W4387014993 hasConcept C163764329 @default.
- W4387014993 hasConcept C203014093 @default.
- W4387014993 hasConcept C2775915377 @default.
- W4387014993 hasConcept C2775934546 @default.
- W4387014993 hasConcept C2776194381 @default.
- W4387014993 hasConcept C2777624698 @default.
- W4387014993 hasConcept C2778075117 @default.
- W4387014993 hasConcept C2778236600 @default.
- W4387014993 hasConcept C2779252107 @default.
- W4387014993 hasConcept C2779305546 @default.
- W4387014993 hasConcept C2780439664 @default.
- W4387014993 hasConcept C2781207856 @default.
- W4387014993 hasConcept C3019965523 @default.
- W4387014993 hasConcept C71924100 @default.
- W4387014993 hasConcept C74864618 @default.
- W4387014993 hasConcept C98246554 @default.
- W4387014993 hasConceptScore W4387014993C142724271 @default.
- W4387014993 hasConceptScore W4387014993C159654299 @default.
- W4387014993 hasConceptScore W4387014993C163764329 @default.
- W4387014993 hasConceptScore W4387014993C203014093 @default.
- W4387014993 hasConceptScore W4387014993C2775915377 @default.
- W4387014993 hasConceptScore W4387014993C2775934546 @default.
- W4387014993 hasConceptScore W4387014993C2776194381 @default.
- W4387014993 hasConceptScore W4387014993C2777624698 @default.
- W4387014993 hasConceptScore W4387014993C2778075117 @default.
- W4387014993 hasConceptScore W4387014993C2778236600 @default.
- W4387014993 hasConceptScore W4387014993C2779252107 @default.
- W4387014993 hasConceptScore W4387014993C2779305546 @default.
- W4387014993 hasConceptScore W4387014993C2780439664 @default.
- W4387014993 hasConceptScore W4387014993C2781207856 @default.
- W4387014993 hasConceptScore W4387014993C3019965523 @default.
- W4387014993 hasConceptScore W4387014993C71924100 @default.
- W4387014993 hasConceptScore W4387014993C74864618 @default.
- W4387014993 hasConceptScore W4387014993C98246554 @default.
- W4387014993 hasIssue "3" @default.
- W4387014993 hasLocation W43870149931 @default.
- W4387014993 hasOpenAccess W4387014993 @default.
- W4387014993 hasPrimaryLocation W43870149931 @default.
- W4387014993 hasRelatedWork W1969074690 @default.
- W4387014993 hasRelatedWork W1994829202 @default.
- W4387014993 hasRelatedWork W1996086845 @default.
- W4387014993 hasRelatedWork W1999677706 @default.
- W4387014993 hasRelatedWork W2005021325 @default.
- W4387014993 hasRelatedWork W2012923237 @default.
- W4387014993 hasRelatedWork W2079464368 @default.
- W4387014993 hasRelatedWork W2119552335 @default.
- W4387014993 hasRelatedWork W2122130153 @default.
- W4387014993 hasRelatedWork W4297934026 @default.
- W4387014993 hasVolume "89" @default.
- W4387014993 isParatext "false" @default.
- W4387014993 isRetracted "false" @default.
- W4387014993 workType "article" @default.