FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387036769> ?p ?o ?g. }

• W4387036769 abstract "Abstract Background Revascularization and restoration of normal pulp-dentin complex are important for tissue-engineered pulp regeneration. Recently, a unique periodontal tip-like endothelial cells subtype (POTCs) specialized to dentinogenesis was identified. We have confirmed that TPPU, a soluble epoxide hydrolase (sEH) inhibitor targeting EETs metabolism, promotes bone growth and regeneration by angiogenesis and osteogenesis coupling. We hypothesized that TPPU could also promote revascularization and induce POTCs to contribute to pulp-dentin complex regeneration. Here, we in vitro and in vivo characterized the potential effect of TPPU on the coupling of angiogenesis and odontogenesis and investigated the relevant mechanism, providing new ideas for pulp-dentin regeneration by targeting sEH. Methods In vitro , the effects of TPPU on the proliferation, migration, and angiogenesis of dental pulp stem cells (DPSCs), human umbilical vein endothelial cells (HUVECs) and cocultured DPSCs and HUVECs were detected using CCK8, wound healing, transwell, tube formation and RT-qPCR. In vivo , Matrigel plug assay was performed to outline the roles of TPPU in revascularization and survival of grafts. Then we characterized the VEGFR2 + POTCs around odontoblast layer in the molar of pups from C57BL/6 female mice gavaged with TPPU. Finally, the root segments with DPSCs mixed Matrigel were implanted subcutaneously in BALB/c nude mice treated with TPPU and the root grafts were isolated for histological staining. Results In vitro , TPPU significantly promoted the migration and tube formation capability of cocultured DPSCs and HUVECs. ALP and ARS staining and RT-qPCR showed that TPPU promoted the osteogenic and odontogenic differentiation of cultured cells, treatment with an anti-TGF-β blocking antibody abrogated this effect. Knockdown of HIF-1α in HUVECs significantly reversed the effect of TPPU on the expression of angiogenesis, osteogenesis and odontogenesis-related genes in cocultured cells. Matrigel plug assay showed that TPPU increased VEGF/VEGFR2-expressed cells in transplanted grafts. TPPU contributed to angiogenic-odontogenic coupling featured by increased VEGFR2 + POTCs and odontoblast maturation during early dentinogenesis in molar of newborn pups from C57BL/6 female mice gavaged with TPPU. TPPU induced more dental pulp-like tissue with more vessels and collagen fibers in transplanted root segment. Conclusions TPPU promotes revascularization of dental pulp regeneration by enhancing migration and angiogenesis of HUVECs, and improves odontogenic differentiation of DPSCs by TGF-β. TPPU boosts the angiogenic–odontogenic coupling by enhancing VEGFR2 + POTCs meditated odontoblast maturation partly via upregulating HIF-1α, which contributes to increasing pulp-dentin complex for tissue-engineered pulp regeneration." @default.
• W4387036769 created "2023-09-27" @default.
• W4387036769 creator A5021541475 @default.
• W4387036769 creator A5030225561 @default.
• W4387036769 creator A5033259787 @default.
• W4387036769 creator A5036389767 @default.
• W4387036769 creator A5047567585 @default.
• W4387036769 creator A5065312383 @default.
• W4387036769 creator A5066652367 @default.
• W4387036769 creator A5074028169 @default.
• W4387036769 date "2023-09-26" @default.
• W4387036769 modified "2023-09-27" @default.
• W4387036769 title "Inhibition of soluble epoxide hydrolase induces POTCs to enhance the dentin-pulp complex regeneration mediated by crosstalk between vascular endothelial cells and dental pulp stem cells" @default.
• W4387036769 cites W1963907322 @default.
• W4387036769 cites W1991969740 @default.
• W4387036769 cites W2004193154 @default.
• W4387036769 cites W2038019472 @default.
• W4387036769 cites W2041217026 @default.
• W4387036769 cites W2041345281 @default.
• W4387036769 cites W2048609378 @default.
• W4387036769 cites W2076424556 @default.
• W4387036769 cites W2149507412 @default.
• W4387036769 cites W2154439651 @default.
• W4387036769 cites W2166573361 @default.
• W4387036769 cites W2312929775 @default.
• W4387036769 cites W2473264262 @default.
• W4387036769 cites W2510606237 @default.
• W4387036769 cites W2567622903 @default.
• W4387036769 cites W2582495616 @default.
• W4387036769 cites W2612645272 @default.
• W4387036769 cites W2704792100 @default.
• W4387036769 cites W2751254124 @default.
• W4387036769 cites W2757184482 @default.
• W4387036769 cites W2792025535 @default.
• W4387036769 cites W2794587232 @default.
• W4387036769 cites W2810046645 @default.
• W4387036769 cites W2883878261 @default.
• W4387036769 cites W2884393110 @default.
• W4387036769 cites W2888250189 @default.
• W4387036769 cites W2899343817 @default.
• W4387036769 cites W3028003900 @default.
• W4387036769 cites W3155980170 @default.
• W4387036769 cites W3188990633 @default.
• W4387036769 cites W4220943961 @default.
• W4387036769 cites W4225898613 @default.
• W4387036769 cites W4284900093 @default.
• W4387036769 cites W4296824571 @default.
• W4387036769 cites W4315928310 @default.
• W4387036769 cites W4362584179 @default.
• W4387036769 cites W4379184516 @default.
• W4387036769 doi "https://doi.org/10.21203/rs.3.rs-3188410/v1" @default.
• W4387036769 hasPublicationYear "2023" @default.
• W4387036769 type Work @default.
• W4387036769 citedByCount "0" @default.
• W4387036769 crossrefType "posted-content" @default.
• W4387036769 hasAuthorship W4387036769A5021541475 @default.
• W4387036769 hasAuthorship W4387036769A5030225561 @default.
• W4387036769 hasAuthorship W4387036769A5033259787 @default.
• W4387036769 hasAuthorship W4387036769A5036389767 @default.
• W4387036769 hasAuthorship W4387036769A5047567585 @default.
• W4387036769 hasAuthorship W4387036769A5065312383 @default.
• W4387036769 hasAuthorship W4387036769A5066652367 @default.
• W4387036769 hasAuthorship W4387036769A5074028169 @default.
• W4387036769 hasBestOaLocation W43870367691 @default.
• W4387036769 hasConcept C150903083 @default.
• W4387036769 hasConcept C171056886 @default.
• W4387036769 hasConcept C181199279 @default.
• W4387036769 hasConcept C185592680 @default.
• W4387036769 hasConcept C18605011 @default.
• W4387036769 hasConcept C188442228 @default.
• W4387036769 hasConcept C198826908 @default.
• W4387036769 hasConcept C199343813 @default.
• W4387036769 hasConcept C207001950 @default.
• W4387036769 hasConcept C2520015 @default.
• W4387036769 hasConcept C2777400515 @default.
• W4387036769 hasConcept C2778608917 @default.
• W4387036769 hasConcept C2779263046 @default.
• W4387036769 hasConcept C2780394083 @default.
• W4387036769 hasConcept C502942594 @default.
• W4387036769 hasConcept C55493867 @default.
• W4387036769 hasConcept C69103154 @default.
• W4387036769 hasConcept C71924100 @default.
• W4387036769 hasConcept C86803240 @default.
• W4387036769 hasConcept C95444343 @default.
• W4387036769 hasConceptScore W4387036769C150903083 @default.
• W4387036769 hasConceptScore W4387036769C171056886 @default.
• W4387036769 hasConceptScore W4387036769C181199279 @default.
• W4387036769 hasConceptScore W4387036769C185592680 @default.
• W4387036769 hasConceptScore W4387036769C18605011 @default.
• W4387036769 hasConceptScore W4387036769C188442228 @default.
• W4387036769 hasConceptScore W4387036769C198826908 @default.
• W4387036769 hasConceptScore W4387036769C199343813 @default.
• W4387036769 hasConceptScore W4387036769C207001950 @default.
• W4387036769 hasConceptScore W4387036769C2520015 @default.
• W4387036769 hasConceptScore W4387036769C2777400515 @default.
• W4387036769 hasConceptScore W4387036769C2778608917 @default.
• W4387036769 hasConceptScore W4387036769C2779263046 @default.
• W4387036769 hasConceptScore W4387036769C2780394083 @default.
• W4387036769 hasConceptScore W4387036769C502942594 @default.
• W4387036769 hasConceptScore W4387036769C55493867 @default.

• next