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- W4387050640 abstract "The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies." @default.
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- W4387050640 date "2023-12-01" @default.
- W4387050640 modified "2023-10-11" @default.
- W4387050640 title "Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis" @default.
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- W4387050640 doi "https://doi.org/10.1016/j.xpro.2023.102618" @default.
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