FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387060520> ?p ?o ?g. }

• W4387060520 abstract "Abstract Stem cell-derived organoid cultures have emerged as attractive experimental models for infection biology research regarding various types of gastro-intestinal pathogens and host species. However, the large size of infectious nematode larvae and the closed structure of 3-dimensional organoids often hinder studies of the natural route of infection. To enable easy administration to the apical surface of the epithelium, organoids from the equine small intestine, i.e. enteroids, were in the present study used to establish epithelial monolayer cultures. These monolayers were functionally tested by stimulation with IL-4 and IL-13, and/or exposure to infectious stage larvae of the equine nematodes Parascaris univalens , cyathostominae and/or Strongylus vulgaris. Effects were recorded using transcriptional analysis combined with histochemistry, immunofluorescence-, live-cell- and scanning electron microscopy. These analyses revealed heterogeneous monolayers containing both immature and differentiated cells including tuft cells and mucus-producing goblet cells. Stimulation with IL-4/IL-13 increased tuft- and goblet cell differentiation as demonstrated by the expression of DCLK1 and MUC2. In these cytokine-primed monolayers, the expression of MUC2 was further promoted by co-culture with P. univalens . Moreover, live-cell imaging revealed morphological alterations of the epithelial cells following exposure to larvae even in the absence of cytokine stimulation. Thus, the present work describes the design, characterization and usability of an experimental model representing the equine nematode-infected small intestinal epithelium. The presence of tuft cells and goblet cells whose mucus production is affected by Th2 cytokines and/or the presence of larvae opens up for mechanistic studies of the casual interactions between nematodes and the equine intestinal mucosa." @default.
• W4387060520 created "2023-09-27" @default.
• W4387060520 creator A5026366428 @default.
• W4387060520 creator A5035613471 @default.
• W4387060520 creator A5040591246 @default.
• W4387060520 creator A5047731948 @default.
• W4387060520 creator A5068145506 @default.
• W4387060520 creator A5071212554 @default.
• W4387060520 creator A5080303392 @default.
• W4387060520 creator A5092946309 @default.
• W4387060520 date "2023-09-26" @default.
• W4387060520 modified "2023-09-27" @default.
• W4387060520 title "Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection" @default.
• W4387060520 cites W2025659071 @default.
• W4387060520 cites W2036295062 @default.
• W4387060520 cites W2072965356 @default.
• W4387060520 cites W2092842402 @default.
• W4387060520 cites W2138581811 @default.
• W4387060520 cites W2162098634 @default.
• W4387060520 cites W2167279371 @default.
• W4387060520 cites W2191813810 @default.
• W4387060520 cites W2233158352 @default.
• W4387060520 cites W2278768857 @default.
• W4387060520 cites W2610568155 @default.
• W4387060520 cites W2744610687 @default.
• W4387060520 cites W2792514364 @default.
• W4387060520 cites W2792848680 @default.
• W4387060520 cites W2792969305 @default.
• W4387060520 cites W2808066902 @default.
• W4387060520 cites W2896466964 @default.
• W4387060520 cites W2904869442 @default.
• W4387060520 cites W2917132599 @default.
• W4387060520 cites W2951791227 @default.
• W4387060520 cites W2958375893 @default.
• W4387060520 cites W3011320584 @default.
• W4387060520 cites W3087140673 @default.
• W4387060520 cites W3088190727 @default.
• W4387060520 cites W3133123878 @default.
• W4387060520 cites W3133787226 @default.
• W4387060520 cites W3154779742 @default.
• W4387060520 cites W3185018810 @default.
• W4387060520 cites W3194498307 @default.
• W4387060520 cites W3197360565 @default.
• W4387060520 cites W3198647108 @default.
• W4387060520 cites W3200210518 @default.
• W4387060520 cites W3206497217 @default.
• W4387060520 cites W3206693498 @default.
• W4387060520 cites W3211767641 @default.
• W4387060520 cites W4200135473 @default.
• W4387060520 cites W4213237714 @default.
• W4387060520 cites W4214843300 @default.
• W4387060520 cites W4220732182 @default.
• W4387060520 cites W4220821901 @default.
• W4387060520 cites W4220953001 @default.
• W4387060520 cites W4283718648 @default.
• W4387060520 cites W4285808873 @default.
• W4387060520 cites W4307902804 @default.
• W4387060520 cites W4313314186 @default.
• W4387060520 cites W4323666243 @default.
• W4387060520 cites W4368617550 @default.
• W4387060520 doi "https://doi.org/10.21203/rs.3.rs-3342735/v1" @default.
• W4387060520 hasPublicationYear "2023" @default.
• W4387060520 type Work @default.
• W4387060520 citedByCount "0" @default.
• W4387060520 crossrefType "posted-content" @default.
• W4387060520 hasAuthorship W4387060520A5026366428 @default.
• W4387060520 hasAuthorship W4387060520A5035613471 @default.
• W4387060520 hasAuthorship W4387060520A5040591246 @default.
• W4387060520 hasAuthorship W4387060520A5047731948 @default.
• W4387060520 hasAuthorship W4387060520A5068145506 @default.
• W4387060520 hasAuthorship W4387060520A5071212554 @default.
• W4387060520 hasAuthorship W4387060520A5080303392 @default.
• W4387060520 hasAuthorship W4387060520A5092946309 @default.
• W4387060520 hasBestOaLocation W43870605201 @default.
• W4387060520 hasConcept C18903297 @default.
• W4387060520 hasConcept C203014093 @default.
• W4387060520 hasConcept C2775941134 @default.
• W4387060520 hasConcept C2777312293 @default.
• W4387060520 hasConcept C2778690821 @default.
• W4387060520 hasConcept C2781077229 @default.
• W4387060520 hasConcept C28328180 @default.
• W4387060520 hasConcept C31695470 @default.
• W4387060520 hasConcept C529295009 @default.
• W4387060520 hasConcept C54355233 @default.
• W4387060520 hasConcept C86803240 @default.
• W4387060520 hasConcept C95444343 @default.
• W4387060520 hasConceptScore W4387060520C18903297 @default.
• W4387060520 hasConceptScore W4387060520C203014093 @default.
• W4387060520 hasConceptScore W4387060520C2775941134 @default.
• W4387060520 hasConceptScore W4387060520C2777312293 @default.
• W4387060520 hasConceptScore W4387060520C2778690821 @default.
• W4387060520 hasConceptScore W4387060520C2781077229 @default.
• W4387060520 hasConceptScore W4387060520C28328180 @default.
• W4387060520 hasConceptScore W4387060520C31695470 @default.
• W4387060520 hasConceptScore W4387060520C529295009 @default.
• W4387060520 hasConceptScore W4387060520C54355233 @default.
• W4387060520 hasConceptScore W4387060520C86803240 @default.
• W4387060520 hasConceptScore W4387060520C95444343 @default.
• W4387060520 hasLocation W43870605201 @default.
• W4387060520 hasOpenAccess W4387060520 @default.

• next