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- W4387062221 abstract "Chronic functional overload (FO) induced by synergist muscle ablation causes rapid and sustained hypertrophy of rodent hindlimb muscles and a fast-to-slow phenotypic transformation in myosin heavy chain (MHC) isoforms associated with aerobicity and fatigue resistance. PURPOSE: Here, we investigated whether mitochondrial proteins associated with oxidative phosphorylation (OXPHOS) paralleled the phenotypic changes in prolonged FO skeletal muscle. We also determined the expression of two mitochondria-derived peptides, humanin and MOTS-c, that are implicated as having systemic impacts on glucose metabolism and energy expenditure. METHODS: Bilateral FO of the plantaris (PLT) muscle was performed in adult female Sprague Dawley rats (~200 g). Age-matched controls (CON) were used for comparison. After 10 wks, PLT muscles were removed, wet weighed, and frozen in isopentane cooled by liquid nitrogen. MHC isoforms and total protein were isolated using separate homogenization procedures. Mitochondria-associated proteins encoded by genomic and mitochondria DNA were separated using standard SDS-PAGE and expression quantified using ImageJ software. Group comparisons were made using a Student’s t-test. RESULTS: FO absolute (401 mg) and relative masses (1.53 mg/g body mass) were significantly (p < 0.01) greater than CON PLT (295.6 mg and 1.13 mg/g, respectively). No group differences in terminal body mass were observed. CON PLT composition for MHC I, IIa, IIx, and IIb was 5.5, 15.6, 44.2 and 34.7%, respectively. FO PLT muscles exhibited a significant increase (p < 0.01) in MHC types I (11.9%) and IIa (18.2%) and a decrease (p < 0.01) in MHC type IIb (24.1%). Compared to CON, no changes were detected in the expression of nuclear-encoded OXPHOS proteins (ATP5, UQCRC2, SDHB, and NDUFB8) of FO PLT muscles. However, FO mitochondrial-encoded OXPHOS expression increased significantly (p < 0.05) for ATP6 (25% above CON), ND1 (21%), COX1 (35%), COX3 (28%), but not for CYTB (p > 0.05). MOTS-c and humanin expression was significantly elevated above CON in FO PLT by 15 and 8%, respectively (p < 0.05). CONCLUSION: The expected increase in types I and IIa MHC isoform after FO is matched by an increase in mitochondrial- but not nuclear-encoded OXPHOS protein expression, possibly reflecting an increase in mitochondrial DNA copy number." @default.
- W4387062221 created "2023-09-27" @default.
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- W4387062221 date "2023-09-01" @default.
- W4387062221 modified "2023-09-27" @default.
- W4387062221 title "Increased Mitochondrial-encoded Protein Expression Coincides With Mhc Phenotype Shift During Hypertrophy Of Rat Plantaris" @default.
- W4387062221 doi "https://doi.org/10.1249/01.mss.0000981752.09655.68" @default.
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