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- W4387071808 abstract "Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.1" @default.
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- W4387071808 date "2023-12-01" @default.
- W4387071808 modified "2023-10-07" @default.
- W4387071808 title "Using PrISMa to reveal the interactome of the human claudins family" @default.
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- W4387071808 doi "https://doi.org/10.1016/j.xpro.2023.102549" @default.
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