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• W4387076987 abstract "Eradication of HIV-1 latently infected cells is an important issue in HIV treatment. However, there are limited models available to assess therapeutic efficacy in vitro. Here, we present a protocol for establishing a variety of HIV-infected Jurkat cells, including productive and latent status, evaluating the efficacy of antiviral agents, followed by PCR/sequencing-based detection of replication competent HIV provirus. This protocol is useful for optimization of treatment of HIV-1 and provides insights into the mechanisms of clonal selection of heterogeneous HIV-1-infected cells. For complete details on the use and execution of this protocol, please refer to Matsuda et al. (2021).1." @default.
• W4387076987 created "2023-09-28" @default.
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• W4387076987 date "2023-12-01" @default.
• W4387076987 modified "2023-10-15" @default.
• W4387076987 title "Assessing the effects of antiretroviral therapy-latency-reversing agent combination therapy on eradicating replication-competent HIV provirus in a Jurkat cell culture model" @default.
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• W4387076987 doi "https://doi.org/10.1016/j.xpro.2023.102547" @default.
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