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- W4387079350 abstract "This study was conducted to investigate in vitro pro-angiogenic and anti-inflammatory phenotypes and functions, and in vivo efficacy and safety of quality and quantity media-cultured mononuclear cells compared to standard cultured mononuclear cells from peripheral blood of chronic limb threatening ischemia patients with atherosclerotic risk factors. PBMNCs from CLTI patients were cultured in Quality and Quantity (QQ) culture media or standard culture media. Phenotypic analysis of progenitor cells (CD34+CD133+), M2 macrophages (CD206+), and inactivated T regulatory cells (CD4+CD25+CD127+); colony forming assay; and tube formation assay of quality and quantity media-cultured mononuclear cells (QQMNCs) and peripheral blood mononuclear cells (PBMNCs), were conducted. Intramuscular transplantation of QQMNCs or PBMNCs was performed in the ischemic hindlimb model. The clinical appearance of ischemic limbs was observed, and blood flow in ischemic limbs was measured using a laser Doppler perfusion imager. Outcomes were compared between the QQMNC and PBMNC groups. Twenty CLTI patients were included. The mean percentages of CD34+ cells, CD133+ cells, CD34+CD133+ progenitor cells, CD206+ cells, colony-forming cells, and tube formation were significantly higher in the QQMNCs. The mean percentage of CD4+CD25+CD127+ cells was significantly lower in QQMNC. The colony-forming unit count and Dil-acetylated low-density lipoprotein uptake were significantly greater in QQMNC. The clinical appearance of post-QQMNC-injected limbs was less severe than the appearance of post-PBMNC-injected limbs. Limb perfusion was significantly better in the QQMNCs. Proangiogenic and anti-inflammatory phenotypes of MNCs cultured in QQ culture media were reproducible. Intramuscular QQMNC transplantation was safe and resulted in better reperfusion of ischemic hindlimbs compared to PBMNCs." @default.
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- W4387079350 date "2023-09-01" @default.
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- W4387079350 title "The Quality and Quantity media-cultured mononuclear cell transplantation is safe and effective in ischemic hindlimb mouse model" @default.
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- W4387079350 doi "https://doi.org/10.1016/j.jvssci.2023.100129" @default.
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