Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387079461> ?p ?o ?g. }
- W4387079461 endingPage "105296" @default.
- W4387079461 startingPage "105296" @default.
- W4387079461 abstract "3D chromatin organization plays a critical role in regulating gene expression, DNA replication, recombination, and repair. While initially discovered for its role in sister chromatid cohesion, emerging evidence suggests that the cohesin complex (SMC1, SMC3, RAD21, and SA1/SA2), facilitated by NIPBL, mediates topologically associating domains (TADs) and chromatin loops through DNA loop extrusion. However, information on how conformational changes of cohesin-NIPBL drive its loading onto DNA, initiation, and growth of DNA loops is still lacking. In this study, high-speed atomic force microscopy (HS-AFM) imaging reveals that cohesin-NIPBL captures DNA through arm extension, assisted by feet (shorter protrusions), and followed by transfer of DNA to its lower compartment (SMC heads, RAD21, SA1 and NIPBL). While binding at the lower compartment, arm extension leads to the capture of a second DNA segment and the initiation of a DNA loop that is independent of ATP hydrolysis. The feet are likely contributed by the C-terminal domains of SA1 and NIPBL and can transiently bind to DNA to facilitate the loading of the cohesin complex onto DNA. Furthermore, HS-AFM imaging reveals distinct forward and reverse DNA loop extrusion steps by cohesin-NIPBL. These results advance our understanding of cohesin by establishing direct experimental evidence for a multi-step DNA binding mechanism mediated by dynamic protein conformational changes." @default.
- W4387079461 created "2023-09-28" @default.
- W4387079461 creator A5003467017 @default.
- W4387079461 creator A5006271100 @default.
- W4387079461 creator A5013164620 @default.
- W4387079461 creator A5030985240 @default.
- W4387079461 creator A5031802450 @default.
- W4387079461 creator A5032037949 @default.
- W4387079461 creator A5041579082 @default.
- W4387079461 creator A5051576642 @default.
- W4387079461 creator A5060110465 @default.
- W4387079461 creator A5075968667 @default.
- W4387079461 creator A5087013377 @default.
- W4387079461 date "2023-09-01" @default.
- W4387079461 modified "2023-10-14" @default.
- W4387079461 title "High-speed AFM imaging reveals DNA capture and loop extrusion dynamics by cohesin-NIPBL" @default.
- W4387079461 cites W171381810 @default.
- W4387079461 cites W1859117663 @default.
- W4387079461 cites W1941076503 @default.
- W4387079461 cites W1973062929 @default.
- W4387079461 cites W1975542854 @default.
- W4387079461 cites W1992476706 @default.
- W4387079461 cites W2018658338 @default.
- W4387079461 cites W2021486745 @default.
- W4387079461 cites W2022431828 @default.
- W4387079461 cites W2045533286 @default.
- W4387079461 cites W2056482755 @default.
- W4387079461 cites W2064537118 @default.
- W4387079461 cites W2070021921 @default.
- W4387079461 cites W2090037139 @default.
- W4387079461 cites W2099034339 @default.
- W4387079461 cites W2107866789 @default.
- W4387079461 cites W2110691960 @default.
- W4387079461 cites W2117028719 @default.
- W4387079461 cites W2120588322 @default.
- W4387079461 cites W2129515289 @default.
- W4387079461 cites W2131253587 @default.
- W4387079461 cites W2132089243 @default.
- W4387079461 cites W2149015451 @default.
- W4387079461 cites W2161212572 @default.
- W4387079461 cites W2163605769 @default.
- W4387079461 cites W2275797864 @default.
- W4387079461 cites W2340540054 @default.
- W4387079461 cites W2401568148 @default.
- W4387079461 cites W2416877295 @default.
- W4387079461 cites W2426320252 @default.
- W4387079461 cites W2761461280 @default.
- W4387079461 cites W2768736041 @default.
- W4387079461 cites W2787093671 @default.
- W4387079461 cites W2788376770 @default.
- W4387079461 cites W2796109902 @default.
- W4387079461 cites W2798187866 @default.
- W4387079461 cites W2891817481 @default.
- W4387079461 cites W2895074278 @default.
- W4387079461 cites W2899721502 @default.
- W4387079461 cites W2900190809 @default.
- W4387079461 cites W2946010758 @default.
- W4387079461 cites W2951726997 @default.
- W4387079461 cites W2963275370 @default.
- W4387079461 cites W2977744221 @default.
- W4387079461 cites W2989713781 @default.
- W4387079461 cites W2991248607 @default.
- W4387079461 cites W3009665504 @default.
- W4387079461 cites W3023617318 @default.
- W4387079461 cites W3023701433 @default.
- W4387079461 cites W3024121050 @default.
- W4387079461 cites W3025316148 @default.
- W4387079461 cites W3026388278 @default.
- W4387079461 cites W3045191935 @default.
- W4387079461 cites W3046933533 @default.
- W4387079461 cites W3091074861 @default.
- W4387079461 cites W3112767499 @default.
- W4387079461 cites W3119506860 @default.
- W4387079461 cites W3120718977 @default.
- W4387079461 cites W3145811080 @default.
- W4387079461 cites W3153266494 @default.
- W4387079461 cites W3161529933 @default.
- W4387079461 cites W3175930821 @default.
- W4387079461 cites W3184281134 @default.
- W4387079461 cites W3204390956 @default.
- W4387079461 cites W4205107526 @default.
- W4387079461 cites W4205560910 @default.
- W4387079461 cites W4226017322 @default.
- W4387079461 cites W4281793572 @default.
- W4387079461 cites W4293368060 @default.
- W4387079461 cites W4295242589 @default.
- W4387079461 cites W4309413498 @default.
- W4387079461 doi "https://doi.org/10.1016/j.jbc.2023.105296" @default.
- W4387079461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37774974" @default.
- W4387079461 hasPublicationYear "2023" @default.
- W4387079461 type Work @default.
- W4387079461 citedByCount "0" @default.
- W4387079461 crossrefType "journal-article" @default.
- W4387079461 hasAuthorship W4387079461A5003467017 @default.
- W4387079461 hasAuthorship W4387079461A5006271100 @default.
- W4387079461 hasAuthorship W4387079461A5013164620 @default.
- W4387079461 hasAuthorship W4387079461A5030985240 @default.
- W4387079461 hasAuthorship W4387079461A5031802450 @default.