Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387081486> ?p ?o ?g. }
- W4387081486 abstract "As CRISPR technology is promoted to more fine-divided molecular biology applications, its inherent performance finds it increasingly difficult to cope with diverse needs in these different fields, and how to more accurately control the performance has become a key issue to develop CRISPR technology to a new stage. Herein, we propose a CRISPR/Cas12a regulation strategy based on the powerful programmability of nucleic acid nanotechnology. Unlike previous difficult and rigid regulation of core components Cas nuclease and crRNA, only a simple switch of different external RNA accessories is required to change the reaction kinetics or thermodynamics, thereby finely and almost steplessly regulating multi-performance of CRISPR/Cas12a including activity, speed, specificity, compatibility, programmability and sensitivity. In particular, the significantly improved specificity is expected to mark advance the accuracy of molecular detection and the safety of gene editing. In addition, this strategy was applied to regulate the delayed activation of Cas12a, overcoming the compatibility problem of the one-pot assay without any physical separation or external stimulation, and demonstrating great potential for fine-grained control of CRISPR. This simple but powerful CRISPR regulation strategy without any component modification has pioneering flexibility and versatility, and will unlock the potential for deeper applications of CRISPR technology in many finely divided fields." @default.
- W4387081486 created "2023-09-28" @default.
- W4387081486 creator A5011666777 @default.
- W4387081486 creator A5014235192 @default.
- W4387081486 creator A5021687717 @default.
- W4387081486 creator A5027575817 @default.
- W4387081486 creator A5029468709 @default.
- W4387081486 creator A5030222738 @default.
- W4387081486 creator A5031004784 @default.
- W4387081486 creator A5031076949 @default.
- W4387081486 creator A5033131707 @default.
- W4387081486 creator A5035256183 @default.
- W4387081486 creator A5042282225 @default.
- W4387081486 creator A5051447698 @default.
- W4387081486 creator A5066716873 @default.
- W4387081486 creator A5068033289 @default.
- W4387081486 creator A5072011646 @default.
- W4387081486 date "2023-09-27" @default.
- W4387081486 modified "2023-09-29" @default.
- W4387081486 title "Unmodificated stepless regulation of CRISPR/Cas12a multi-performance" @default.
- W4387081486 cites W1617520624 @default.
- W4387081486 cites W1964928696 @default.
- W4387081486 cites W1980301976 @default.
- W4387081486 cites W1993061471 @default.
- W4387081486 cites W2013676609 @default.
- W4387081486 cites W2019682748 @default.
- W4387081486 cites W2096786237 @default.
- W4387081486 cites W2108038398 @default.
- W4387081486 cites W2188223075 @default.
- W4387081486 cites W2316952090 @default.
- W4387081486 cites W2317687374 @default.
- W4387081486 cites W2341061908 @default.
- W4387081486 cites W2410526873 @default.
- W4387081486 cites W2606915822 @default.
- W4387081486 cites W2607086226 @default.
- W4387081486 cites W2754927917 @default.
- W4387081486 cites W2767343818 @default.
- W4387081486 cites W2785793003 @default.
- W4387081486 cites W2786426577 @default.
- W4387081486 cites W2790313560 @default.
- W4387081486 cites W2790790238 @default.
- W4387081486 cites W2810059222 @default.
- W4387081486 cites W2810756255 @default.
- W4387081486 cites W2816361009 @default.
- W4387081486 cites W2901552404 @default.
- W4387081486 cites W2939661038 @default.
- W4387081486 cites W2944263608 @default.
- W4387081486 cites W2974267223 @default.
- W4387081486 cites W2979046344 @default.
- W4387081486 cites W2984059576 @default.
- W4387081486 cites W2992478910 @default.
- W4387081486 cites W2997842553 @default.
- W4387081486 cites W2998106169 @default.
- W4387081486 cites W3017334101 @default.
- W4387081486 cites W3024194273 @default.
- W4387081486 cites W3027680984 @default.
- W4387081486 cites W3037841445 @default.
- W4387081486 cites W3042406794 @default.
- W4387081486 cites W3045235658 @default.
- W4387081486 cites W3047186826 @default.
- W4387081486 cites W3084605929 @default.
- W4387081486 cites W3087332812 @default.
- W4387081486 cites W3087541931 @default.
- W4387081486 cites W3090270817 @default.
- W4387081486 cites W3097643224 @default.
- W4387081486 cites W3104540940 @default.
- W4387081486 cites W3108711749 @default.
- W4387081486 cites W3110932432 @default.
- W4387081486 cites W3123905071 @default.
- W4387081486 cites W3126347114 @default.
- W4387081486 cites W3138228681 @default.
- W4387081486 cites W3162126409 @default.
- W4387081486 cites W3188079964 @default.
- W4387081486 cites W3190407283 @default.
- W4387081486 cites W3200131276 @default.
- W4387081486 cites W3214357114 @default.
- W4387081486 cites W4200007229 @default.
- W4387081486 cites W4206939642 @default.
- W4387081486 cites W4210330458 @default.
- W4387081486 cites W4220710122 @default.
- W4387081486 cites W4281661400 @default.
- W4387081486 cites W4283219719 @default.
- W4387081486 cites W4284701626 @default.
- W4387081486 cites W4296689706 @default.
- W4387081486 cites W4297264811 @default.
- W4387081486 cites W4306914543 @default.
- W4387081486 cites W4307840375 @default.
- W4387081486 cites W4308773145 @default.
- W4387081486 cites W4316810847 @default.
- W4387081486 cites W4318071567 @default.
- W4387081486 cites W4320032924 @default.
- W4387081486 cites W4324021335 @default.
- W4387081486 cites W4327744893 @default.
- W4387081486 cites W4362523392 @default.
- W4387081486 cites W4362601933 @default.
- W4387081486 cites W4367291436 @default.
- W4387081486 cites W4376454526 @default.
- W4387081486 doi "https://doi.org/10.1093/nar/gkad748" @default.
- W4387081486 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37757856" @default.
- W4387081486 hasPublicationYear "2023" @default.