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- W4387130003 abstract "Abstract:Biocompatible nanoparticles are very popular in health science research. Biomolecule carriers for wound healing and tissue engineering are two main applications among many others. In many instances, these structures come in direct vicinity of cells and govern cell behaviour and responses. In this study, gelatin nano/submicron structures were synthesized by binary nonsolvent aided coacervation (BNAC) method at pH ranging from 3 to 11 with an intention to employ in skin tissue regeneration. Effect of pH over morphology and the surface composition with respect to its ionic composition were studied. Further, the initial toxicity was assessed against peripheral blood mononuclear cells (PBMC). pH 7 was found to be the optimum for synthesis of gelatin nanoparticles (GNPs) with minimum particle size. Positive cell viability of 103.14% for GNPs synthesized at pH 7 was observed. It may be due to the minimum difference between cumulative negative and positive charge (CNCP) ratio of 1.19. Finally, effect of the gelatin nanoparticles over L929 mouse fibroblast cells were assessed through MTT assay. It has resulted in 122.77% cell viability.Keywords: Gelatin nano particleBinary nonsolvent aided coacervation (BNAC) methodCumulative negative and positive charge (CNCP) ratioPeripheral blood mononuclear cell (PBMC)L929 mouse fibroblast cellsDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. Acknowledgements1. We are thankful to Dr. D.S. Kothari Postdoctoral Fellowship, UGC, Govt. of India., for funding the research.2. We are thankful to Late Dr. D. N. Tibarewala (Retd. Professor and Ex. Director), School of Bioscience and Engineering, Jadavpur University, Kolkata-700032 to motivate us guide us as Dr. D. S. Kothari Postdoctoral mentor to initiate the journey in details.FundingThe author(s) reported there is no funding associated with the work featured in this article.Additional informationNotes on contributorsShamayita PatraDr. Shamayita Patra is working as Associate Professor in Shri Vaishnav Institute of Textile Technology at Shri Vaishnav Vidyapeeth Vishwavidyalaya, Indore, India. She has completed her B.Tech in Jute and Fibre Technology from University of Calcutta. She earned her M.Tech in Fibre Science and Technology and Ph.D in Biomedical Engineering from Indian Institute of Technology Delhi (IIT Delhi). She was Dr. D.S. Kothari Postdoctoral fellow from 2013 to 2016. Her areas of research are Medical Textile, Nanomaterials, Soft Tissue Engineering, Natural Fibre, Textile Engineering etc. She has published and presented more than 35 research papers in various National and International Journals and Conferences. She has been awarded two patents and published one patent. Under her supervision presently 2 scholars are pursuing Ph.D research. She has been awarded with IEI Young Engineers Award 2018–19 in the field of Textile Engineering by Institute of Engineers (India), Young Scientist Award by IARDO in 2019 and Gurukul Academic Award 2022 for Teaching and Research excellence in 2022. Experience: 7 years of Teaching and 11 years of research experienceExperience: 7 years of Teaching and 11 years of research experiencePiyali BasakDr. Piyali Basak, M.E, PhD, Director, School of Bioscience and Engineering, Jadavpur University, Kolkata-700032, email- piyalibasak@gmail.com.Experience: more than 10 yearsProfessor Basak is currently the Director of the School of Bioscience and Engineering in Jadavpur University, Kolkata India. She authored more than 70 research and review articles in several international journals and several book chapters. She has got various research award throughout her career. She is currently working on nano-biomaterials, polymer nanocomposites for various applications like drug delivery, wound healing, antimicrobial coatings over surgical instruments, and nano-therapeutics. Dr.Basak is also involved in works related to environmental studies, related to dye degradation and natural product extract for therapeutic uses.Pratik DasMr. Pratik Das, (Research Scholar), School of Bioscience and Engineering, Jadavpur University, Kolkata-700032. email- pratik.me.online@gmail.comMr. Pratik Das did his Masters of Engineering from School of Bioscience and Engineering. Currently, Mr. Das is pursuing his PhD from Jadavpur University. He is working on the field of nano-biomaterials and their biomedical application. He is currently continuing his research in School of Bioscience and Engineering. He has authored or co-authored more than 10 international research and review articles in SCI and Scopus indexed journals. Current research interest of Mr. Das includes Green Synthesis of Silver nanoparticles, Computational Biology, Biomaterials and its application in Biomedical Industry and Synthesis and application of Bio derived carbon Dots.Samrat PaulDr. Samrat Paul, PhD, BIRAC innovation Post-Doctoral Fellow at E- YUVA center, Adamas University. email: paulsamrat.12@gmail.comExperience: more than 2.5 yearsDr. Samrat Paul is currently working as Post-Doctoral Fellow Aka BIRAC innovation Fellow at E- YUVA center, Adamas University since January, 2023. He has 2.5 years of academic experience as Assistant Professor at Department of Biotechnology, Brainware University. He did his MSc in Human Physiology from Calcutta University and completed his PhD in Science (Life science/Biotechnology) from Jadavpur University. He has 9 SCI impact Journal publication and 2 published Patent. He received WB-DST fellowship and RUSA 2.0 Doctorate Fellowship during his PhD time and obtained Best Poster Presentation award in Indian Science Congress, 2019." @default.
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- W4387130003 title "A Novel observation: Effect of Anionic Gelatin Nano Particle on Stromal Cells" @default.
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- W4387130003 doi "https://doi.org/10.1080/09205063.2023.2265129" @default.
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