Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387130360> ?p ?o ?g. }
- W4387130360 abstract "Paclitaxel is an anticancer therapeutic produced by the yew tree. Over the last two decades, a significant bottleneck in the reconstitution of early paclitaxel biosynthesis has been the propensity of heterologously expressed pathway cytochromes P450, including taxadiene 5α-hydroxylase (T5αH), to form multiple products. This diverts metabolic flux away from the paclitaxel precursor, taxadien-5α-ol, thus previous attempts of reconstitution have not yielded sufficient material for characterization, regardless of the heterologous host. Here, we structurally characterized four new products of T5αH, many of which appear to be over-oxidation of the primary mono-oxidized products. By tuning the promoter strength for T5αH expression, levels of these proposed byproducts decrease with a concomitant increase in the accumulation of taxadien-5α-ol by four-fold. This engineered system enabled the reconstitution of a six step biosynthetic pathway to produce isolatable 5α,10β-diacetoxy-taxadien-13α-ol. Furthermore, we showed that this pathway may function as a metabolic network rather than a linear pathway. The engineering of the paclitaxel biosynthetic network demonstrates that Taxus genes can coordinatively function for the biosynthetic production of key early stage paclitaxel intermediates and serves as a crucial platform for the discovery of the remaining biosynthetic genes." @default.
- W4387130360 created "2023-09-29" @default.
- W4387130360 creator A5011726556 @default.
- W4387130360 creator A5045999960 @default.
- W4387130360 creator A5047908856 @default.
- W4387130360 creator A5092959753 @default.
- W4387130360 date "2023-09-28" @default.
- W4387130360 modified "2023-10-18" @default.
- W4387130360 title "Reconstitution of Early Paclitaxel Biosynthetic Network" @default.
- W4387130360 cites W1830282417 @default.
- W4387130360 cites W1965217309 @default.
- W4387130360 cites W1973481954 @default.
- W4387130360 cites W1976365177 @default.
- W4387130360 cites W1990226486 @default.
- W4387130360 cites W1998240064 @default.
- W4387130360 cites W1998858180 @default.
- W4387130360 cites W2001573310 @default.
- W4387130360 cites W2012111825 @default.
- W4387130360 cites W2013251051 @default.
- W4387130360 cites W2026553348 @default.
- W4387130360 cites W2028965340 @default.
- W4387130360 cites W2035680796 @default.
- W4387130360 cites W2050289749 @default.
- W4387130360 cites W2051582324 @default.
- W4387130360 cites W2067769315 @default.
- W4387130360 cites W2070763113 @default.
- W4387130360 cites W2085406594 @default.
- W4387130360 cites W2087271371 @default.
- W4387130360 cites W2097049612 @default.
- W4387130360 cites W2149202606 @default.
- W4387130360 cites W2161699606 @default.
- W4387130360 cites W2166323054 @default.
- W4387130360 cites W2168059482 @default.
- W4387130360 cites W2212394217 @default.
- W4387130360 cites W2270732674 @default.
- W4387130360 cites W2291625402 @default.
- W4387130360 cites W2294225971 @default.
- W4387130360 cites W2300339793 @default.
- W4387130360 cites W2320632410 @default.
- W4387130360 cites W2330181854 @default.
- W4387130360 cites W2369191639 @default.
- W4387130360 cites W2413265783 @default.
- W4387130360 cites W2541859010 @default.
- W4387130360 cites W2556049317 @default.
- W4387130360 cites W2559716871 @default.
- W4387130360 cites W2601550844 @default.
- W4387130360 cites W2753282153 @default.
- W4387130360 cites W2792496404 @default.
- W4387130360 cites W2917202476 @default.
- W4387130360 cites W2944421815 @default.
- W4387130360 cites W2982260601 @default.
- W4387130360 cites W2990866481 @default.
- W4387130360 cites W2993040703 @default.
- W4387130360 cites W2993720587 @default.
- W4387130360 cites W3082772719 @default.
- W4387130360 cites W3087389893 @default.
- W4387130360 cites W3093707140 @default.
- W4387130360 cites W3110830766 @default.
- W4387130360 cites W3127892542 @default.
- W4387130360 cites W4238591244 @default.
- W4387130360 cites W4239270076 @default.
- W4387130360 cites W4294052668 @default.
- W4387130360 cites W4296368399 @default.
- W4387130360 cites W4318264067 @default.
- W4387130360 cites W4323653227 @default.
- W4387130360 doi "https://doi.org/10.1101/2023.09.27.559859" @default.
- W4387130360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37808792" @default.
- W4387130360 hasPublicationYear "2023" @default.
- W4387130360 type Work @default.
- W4387130360 citedByCount "0" @default.
- W4387130360 crossrefType "posted-content" @default.
- W4387130360 hasAuthorship W4387130360A5011726556 @default.
- W4387130360 hasAuthorship W4387130360A5045999960 @default.
- W4387130360 hasAuthorship W4387130360A5047908856 @default.
- W4387130360 hasAuthorship W4387130360A5092959753 @default.
- W4387130360 hasBestOaLocation W43871303601 @default.
- W4387130360 hasConcept C104317684 @default.
- W4387130360 hasConcept C121608353 @default.
- W4387130360 hasConcept C14036430 @default.
- W4387130360 hasConcept C178790620 @default.
- W4387130360 hasConcept C181199279 @default.
- W4387130360 hasConcept C185592680 @default.
- W4387130360 hasConcept C192989942 @default.
- W4387130360 hasConcept C2777292972 @default.
- W4387130360 hasConcept C2778894183 @default.
- W4387130360 hasConcept C2780115692 @default.
- W4387130360 hasConcept C54355233 @default.
- W4387130360 hasConcept C553450214 @default.
- W4387130360 hasConcept C55493867 @default.
- W4387130360 hasConcept C59822182 @default.
- W4387130360 hasConcept C68709404 @default.
- W4387130360 hasConcept C86803240 @default.
- W4387130360 hasConcept C95444343 @default.
- W4387130360 hasConceptScore W4387130360C104317684 @default.
- W4387130360 hasConceptScore W4387130360C121608353 @default.
- W4387130360 hasConceptScore W4387130360C14036430 @default.
- W4387130360 hasConceptScore W4387130360C178790620 @default.
- W4387130360 hasConceptScore W4387130360C181199279 @default.
- W4387130360 hasConceptScore W4387130360C185592680 @default.
- W4387130360 hasConceptScore W4387130360C192989942 @default.