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- W4387135866 abstract "<h3>Introduction/Background</h3> Tumor spheroids in the ascites of high-grade serous cancer (HGSC) patients are poorly described since all research has focused on 3D in vitro models. Our objective was to describe HGSC spheroids in the ascites, with emphasis on their morphological features, cell composition, and PD-1 and PD-L1 expression on spheroid-associated tumor- and immune cells. <h3>Methodology</h3> Patients with histologically confirmed HGSC and present malignant ascites were included in the study. Spheroid density and size in the ascites were assessed from Giemsa smears, the cell composition of spheroid-associated immune cells, tumor cells and endothelial cells, as well as PD-1 and PD-L1 expression were assessed from immunocytochemically stained cell block sections for CD3, CD4, CD8, CD20, CD68, CD34, ERG, PD-1, and PD-L1 (22C3, SP263 and SP142 clones). The analysis of all parameters was performed semi-quantitatively. Obtained data were used to calculate if there is any correlation with progression-free survival (PFS) and overall survival (OS). Furthermore, a cut-off value for low and high density of tumor spheroids in the ascites that could predict better/poorer survival outcome in the patients was determined. Survival analysis was based on a 3.5-year patient follow-up. <h3>Results</h3> Forty-seven patients were included in the study. Three different spheroid-size categories were observed in the ascites, (small, medium, large) but the size did not correlate with survival outcome. However, a 10% cut-off for spheroid density was established, that significantly predicted better/worse PFS and OS. Furthermore, our results confirmed the presence of spheroid-associated lymphocytes (mostly T lymphocytes), macrophages, and endothelial cells. PD-1 expression was observed only on spheroid-associated lymphocytes. PD-L1 expression was seen on both spheroid-associated tumor cells, lymphocytes, and macrophages assessed by 22C3 and SP263 clones but not with the SP142 clone. <h3>Conclusion</h3> Our results highlight the potential of cytopathology to analyze ascites spheroids as potential predictive markers for HGSC patients. <h3>Disclosures</h3> The authors made no disclosures. The study was funded by the research program P3–0289 of the Slovenian Research Agency." @default.
- W4387135866 created "2023-09-29" @default.
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- W4387135866 date "2023-09-01" @default.
- W4387135866 modified "2023-10-17" @default.
- W4387135866 title "#271 Characterization of tumor spheroids from high-grade serous ovarian cancer based on morphological features and cell composition" @default.
- W4387135866 doi "https://doi.org/10.1136/ijgc-2023-esgo.551" @default.
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