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• W4387138094 abstract "Abstract Aflatoxins (AFs) are secondary fungal metabolites that contaminate common food crops and are harmful to humans and animals. The ability to degrade or remove aflatoxins from common feed commodities will improve health standards and counter the economic drain inflicted by AF contamination. Bioremediation is a promising solution to AF contamination because of its low cost and few undesired environmental side-effects. Identifying new degrader species is highly beneficial in that it can offer alternatives to overcome the limitations of existing biodegraders, such as narrow working conditions and low degradation rates. Here, we screen several environmental isolates for their AF detoxification ability, using aflatoxin G 2 . We use different carbon sources (glucose and starch) as isolation and culturing media to examine the effect of the environment on degradation ability. Strains isolated in media with starch as the primary carbon source showed a higher percentage of good AF degraders, 16% compared to 2% when glucose was the primary carbon source. Additionally, the majority of species isolated in glucose medium exhibited improved degradation efficiency when moved into starch medium, with one isolate improving degradation levels from 30% to 70%. Our starch screen also revealed three previously unidentified AF degrader bacterial species. Good aflatoxin G 2 degraders also appear to perform well against aflatoxin B 1 . Overall, for AF degradation, starch medium expedites the screening process and generally improves the performance of isolates. We thus propose that using starch as the carbon source is a promising means to identify new AF degraders in the environment." @default.
• W4387138094 created "2023-09-29" @default.
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• W4387138094 date "2023-09-27" @default.
• W4387138094 modified "2023-10-05" @default.
• W4387138094 title "Starch can expedite the screening for bacterial aflatoxin degraders" @default.
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• W4387138094 doi "https://doi.org/10.1101/2023.09.27.559811" @default.
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