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- W4387140094 abstract "The pre-treatment of seeds prior to processing is gaining attention as alternative ways of modifying properties of foods. In this work, the extrusion and germination of quinoa seeds were evaluated for their effect on protein profile, alcalase hydrolysis and the antioxidant capacity (AOC) of peptide fractions (>10, 3–10 and <3 kDa). The proteins in extruded (EQ), germinated (GQ) and unprocessed (UQ) seeds were extracted, hydrolysed and fractionated by ultrafiltration. An SDS-PAGE protein profile showed that the pre-treatments partially hydrolysed high-molecular-weight proteins (75–100 kDa) into low-molecular-weight polypeptides, and chenopodin was unaltered. The hydrolysis degree in hydrolysates reached 38.38% for UQ seeds, 31.85% for EQ seeds and 30.09% for GQ seeds. Compared to the UQ hydrolysate, the extrusion and germination significantly improved (p < 0.05) the AOC of the <3 kDa fraction by 61.49% (EQ) and 38.11% (GQ) and the 3–103–10 kDa fraction by 130.98% (EQ) and 57.71% (GQ). The pre-treatment of seeds before protein extraction and hydrolysis modified the peptide profile with improved antiradical activity after alcalase hydrolysis. This study highlights the use of mild pre-treatments applied to quinoa seeds as a way to modify proteins and obtain hydrolysates with enhanced bioactivity." @default.
- W4387140094 created "2023-09-29" @default.
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- W4387140094 date "2023-09-28" @default.
- W4387140094 modified "2023-10-18" @default.
- W4387140094 title "Effect of Pre-Treatment of Quinoa Seeds on Alcalase Hydrolysis and Antiradical Activity of Peptides Fractions" @default.
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- W4387140094 doi "https://doi.org/10.3390/blsf2023025008" @default.
- W4387140094 hasPublicationYear "2023" @default.
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