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• W4387141282 abstract "Full text Figures and data Side by side Abstract eLife assessment Introduction Results Discussion Materials and methods Data availability References Peer review Author response Article and author information Metrics Abstract The study of color patterns in the animal integument is a fundamental question in biology, with many lepidopteran species being exemplary models in this endeavor due to their relative simplicity and elegance. While significant advances have been made in unraveling the cellular and molecular basis of lepidopteran pigmentary coloration, the morphogenesis of wing scale nanostructures involved in structural color production is not well understood. Contemporary research on this topic largely focuses on a few nymphalid model taxa (e.g., Bicyclus, Heliconius), despite an overwhelming diversity in the hierarchical nanostructural organization of lepidopteran wing scales. Here, we present a time-resolved, comparative developmental study of hierarchical scale nanostructures in Parides eurimedes and five other papilionid species. Our results uphold the putative conserved role of F-actin bundles in acting as spacers between developing ridges, as previously documented in several nymphalid species. Interestingly, while ridges are developing in P. eurimedes, plasma membrane manifests irregular mesh-like crossribs characteristic of Papilionidae, which delineate the accretion of cuticle into rows of planar disks in between ridges. Once the ridges have grown, disintegrating F-actin bundles appear to reorganize into a network that supports the invagination of plasma membrane underlying the disks, subsequently forming an extruded honeycomb lattice. Our results uncover a previously undocumented role for F-actin in the morphogenesis of complex wing scale nanostructures, likely specific to Papilionidae. eLife assessment This important study reports how swallowtail butterflies pattern structures composed of chitin at the nanometer scale to produce structural colors. The work uses state-of-the-art microscopy techniques to convincingly show that F-actin is utilized in these butterflies in a novel way to produce structure, paving the way for further studies on growth regulation leading to precise ultrastructures and structural colors. https://doi.org/10.7554/eLife.89082.3.sa0 About eLife assessments Introduction The patterning and coloration of butterfly wings have been a paradigmatic research focus due to their fundamental role in signaling and crypsis (Beldade and Brakefield, 2002; Sweeney et al., 2003; Tsai et al., 2020). Significant advances have been made recently in identifying the cellular and molecular basis of lepidopteran pigmentary coloration (Matsuoka and Monteiro, 2018; Nadeau et al., 2016; Nishikawa et al., 2015; Reed et al., 2011; Van Belleghem et al., 2023; Zhang et al., 2017a). A small number of regulatory genes have been found to exert significant influence on the synthesis and spatial expression of pigments, as well as regulating cuticle deposition, thereby affecting the overall scale morphology (e.g., Banerjee and Monteiro, 2022; Brien et al., 2022; Ficarrotta et al., 2022; Matsuoka and Monteiro, 2018; Prakash et al., 2022; Van Belleghem et al., 2023; Wee et al., 2022). For instance, suppression of the optix gene has been found to tune lower lamina thickness, inducing iridescent structural coloration and affecting cuticular coverage of the upper lamina (Wasik et al., 2014; Zhang et al., 2017b). Building on the classic studies on cellular organization of lepidopteran scales (Ghiradella, 1974; Ghiradella, 1985; Ghiradella, 1989; Ghiradella and Radigan, 1976; Overton, 1966), a few recent studies utilized advances in microscopy techniques to interrogate the formation of longitudinal ridges (Day et al., 2019; Dinwiddie et al., 2014; McDougal et al., 2021). These insights are, however, limited to ridge formation on the scale surface. Moreover, contemporary research on scale cell development focuses on just a few model taxa (Bicyclus, Precis, Heliconius, Vanessa) all from the family of Nymphalidae (Beldade and Brakefield, 2002; Brien et al., 2022; Day et al., 2019; Dinwiddie et al., 2014; Matsuoka and Monteiro, 2018; Nadeau et al., 2016; Pomerantz et al., 2020; Prakash et al., 2022 ; Reed et al., 2011; Sweeney et al., 2003; Van Belleghem et al., 2023; Wasik et al., 2014; Zhang et al., 2017b), despite an overwhelming diversity in the hierarchical organization of scale nanostructures across Lepidoptera (Ghiradella, 1984; Ghiradella, 1985; Ghiradella, 1989; Prum et al., 2006). A rare exception is the recent work on pierid butterfly Colias identifying bric à brac as a genetic switch that suppresses UV-reflecting Christmas tree-like ridge gratings in males (Ficarrotta et al., 2022), even though the precise development of the ridge grating is still unresolved (Ghiradella, 1974). Deciphering the cellular and developmental basis of scale organization in beyond just a few model lepidopterans is also highly relevant to current challenges in the synthesis of complex sub-micron and micron-scale hierarchical nanostructures, and could inspire novel biomimetic routes to fabricate multifunctional nanomaterials (Kolle et al., 2010; McDougal et al., 2019; Pokroy et al., 2009; Potyrailo et al., 2007; Siddique et al., 2017; Wilts et al., 2019). The bauplan of lepidopteran wing scales consists an ornamented upper lamina over a relatively unstructured basal lamina, supported by arches with pillar-like struts called trabeculae (Ghiradella, 1984; Figure 1—figure supplement 1). The upper lamina is comprised of longitudinal ridges with transverse crossribs framing a set of rectilinear windows. These windows typically open into the interior lumen of the scale cell, but can also be covered by a thin layer of cuticular lamina (Ghiradella, 1984). The sides of the ridges feature microribs – fine flute-like stripes visible at higher magnifications under an electron microscope (Figure 1—figure supplement 1). Among all Lepidoptera families, swallowtail butterflies (Papilionidae) not only encompasses the known diversity of scale nanostructure, but also exhibit some of the most complex hierarchical morphologies found in nature (Ghiradella, 1984; Ghiradella, 1985; Ghiradella, 1989; Prum et al., 2006; Saranathan et al., 2010; Figure 1—figure supplement 1). Despite having a few model species (e.g., Papilio xuthus, Papilio polytes, and Papilio machaon), the focus of the current research on papilionids is limited to the molecular basis of mimicry of pigment patterns (e.g., Nishikawa et al., 2015; Yoda et al., 2021). The wing scales of papilionid species (e.g., Parides sesostris, Papilio nireus) exhibit irregular veins or mesh-like crossribs, often with an underlying honeycomb-like lattice of cuticular walls enclosing columnar pores (Ghiradella, 1984; Ghiradella, 1985; Ghiradella and Radigan, 1976; Prum et al., 2006; Saranathan et al., 2010; Wilts et al., 2014; Figure 1 and Figure 1—figure supplement 1). In the following, we use ‘honeycomb lattices’ to refer to irregular crossribs with lattice walls extending into the interior lumen (e.g., green scales in P. sesostris, Parides arcas, and Parides eurimedes), and ‘irregular crossribs’ for crossribs that lack such extruded cuticular walls. In any case, these irregular crossribs are an autapomorphy of papilionid butterflies (Ghiradella, 1984; Ghiradella, 1985; Ghiradella and Radigan, 1976) that are thought to increase the surface area over which pigments such as papiliochromes can be deposited (Wilts et al., 2012), and likely play a role in thermoregulation as they are efficient absorbers of solar radiation (Siddique et al., 2017). Figure 1 with 1 supplement see all Download asset Open asset Hierarchical nanostructure of wing scales in Parides eurimedes (Papilionidae). (A) Adult male with structurally colored green patches on their dorsal forewings. Credit: iDigBio YPM Ent 433579. (B) Scanning electron microscopy (SEM) top view and (C) Focused ion beam (FIB)-SEM cross-sectional image of an adult green scale showing ridges and honeycomb lattice ending in trabeculae on top of a perforated multilayer lattice. Scale bars: (A) 1 cm, (B, C) 1 µm. Here, we use scanning electron microscopy (SEM), conventional confocal and super-resolution structured illumination microscopy (3D-SIM) to study the time-resolved development of hierarchical scale nanostructures in wing scales of six papilionid species, focusing mainly on P. eurimedes. We discovered that following ridge formation, rows of chitinous disks form in between the ridges, followed by disintegration of F-actin that reorganizes into a mesh-like network. This network likely acts as a template for subsequent formation of honeycomb lattices by driving plasma membrane invagination into the scale lumen. Our study uncovers a novel process of wing scale nanostructure formation in papilionid species. Results Wing scale ridge formation is conserved in Papilionidae We comparatively studied the wing scale growth in P. polytes and P. eurimedes, which have irregular crossribs and honeycomb lattices, respectively. Early stages of scale cell growth are as previously documented in several nymphalid species (Day et al., 2019; Dinwiddie et al., 2014; Ghiradella, 1974; Pomerantz et al., 2020). Wheat germ agglutinin (WGA) stains the plasma membrane during early stages of scale development in P. eurimedes. Scale cells from relatively young pupae at 38% development resemble elongated buds containing densely packed polymerizing F-actin filaments (Figure 2—figure supplement 1A–A'' and B–B''). At 43% development, F-actin filaments form thicker bundles that extend down the full length of scale cells (Figure 2—figure supplement 1C'), laying down a scaffold that determines the eventual position of ridges (Dinwiddie et al., 2014). WGA stains pleating membranes (longitudinal striations) in between adjacent rows of F-actin bundles (Figure 2—figure supplement 1C–C'' and D–D''). Around 48–50% development, the developing ridges can be more clearly discerned in between F-actin bundles (Figure 2—figure supplement 1E–E'' and Figure 4—figure supplement 6D–D'' and E-E''). At this stage, there also appear to be irregular gaps in WGA staining in between the ridges of P. eurimedes scales (Figure 2—figure supplement 1E). These vein-like features resemble the irregular crossribs seen in adult wing scales (Figure 1B and C and Figure 4—figure supplement 6B and C). Crossrib and cuticular disk formation in P. eurimedes We use lectin and membrane stains to follow plasma membrane topology and cuticle deposition during scale cell maturation (Figure 2 and Figure 2—figure supplements 2–5). At 48% development, plasma membrane appears mottled while WGA predominantly stains longitudinal striations that will become ridges (Figure 2A–C and Figure 2—figure supplement 2B–B''). In cross-sections, both stains appear to be in close contact at the periphery of the cell, with plasma membrane underlying the cuticular layer (Figure 2C and Figure 2—figure supplement 2C–C''). This observation is expected as the cuticle of extracellular origin is being deposited onto the scale cell membrane. At 52% pupal development, plasma membrane shows distinct pleating corresponding to the developing ridges, with irregular crossribs in between (Figure 2A'–C' and Figure 2—figure supplement 3A'–C'). The bulk of cuticle remains above the plasma membrane at this stage (Figure 2C' and Figure 2—figure supplement 3C''). At around 62% pupal development, ridges appear to have grown to their near-final configuration while lectin staining reveals planar, disk-like cuticular features (hereafter cuticular disks) arranged in rows in between the ridges (Figure 2A''–C'' and A'''–C''' and Figure 2—figure supplements 4 and 5). Figure 2 with 5 supplements see all Download asset Open asset Morphogenetic time series of the development of cuticular disks in pupal P. eurimedes dorsal forewing scales, acquired with a 100× confocal microscope. AF-555 wheat germ agglutinin (WGA) (yellow) stains chitin and CellMask (magenta) stains plasma membrane. By 52% development, hollow vein-like crossribs appear on plasma membrane in between ridges that serve as a scaffold for cuticle accretion. As scales mature, more cuticle is deposited into these rows of disks bounded by plasma membrane. See also Figure 2—figure supplements 3–5. (B–B''') Closeup views of (A–A'''). (C–C''') xz cross-sections of the scale at locations marked with gray lines in (A–A''') reveal the planar aspect of the cuticular disks. Yellow regions of interest (ROIs) in (C'–C''') correspond to those in (B'–B'''). Scale bars (A–A''') 5 µm, (B–B''' and C–C''') 2 µm. Dynamics of F-actin reorganization in P. eurimedes We also co-stain chitin and F-actin on the developing pupal scales to investigate the role of cytoskeleton in scale nanostructure formation. Interestingly, as cuticular disks of various sizes are forming in between ridges at 57% development, F-actin bundles are breaking down into short fibrils distributed around the disks (Figure 3B–C, Figure 3—figure supplement 1, and Figure 3—video 1). As the scales became more sclerotized and flattened out later in development (at 67%), disintegrating F-actin appears to reorganize and associate more clearly with cuticular disks, which now appear elongated and slightly tubular in cross-sectional view (Figure 3A'–C', Figure 3—figure supplement 2, and Figure 3—video 2). By 76% development, the cuticular disks appeared hollow and are surrounded by distinct bulb-like structures formed by the disintegrated F-actin bundles (Figure 3A''–C'', Figure 3—figure supplement 3, and Figure 3—video 3). Figure 3 with 6 supplements see all Download asset Open asset Morphogenetic time series of the development of columnar honeycomb lattice in pupal P. eurimedes dorsal forewing scale cells acquired with super-resolution lattice structured illumination microscopy (SIM). AF-555 wheat germ agglutinin (WGA) (yellow) stains chitin and AF-647 phalloidin (magenta) stains F-actin. AF-555 WGA shows a gradual evolution of the cuticular disks from filled-in planar to hollow tubular outgrowths. The disintegrating F-actin bundles show evidence of reorganization from linear to reticulated features with ring-like cross-sections. See also Figure 3—figure supplements 1–3. (B–B'') Closeup views of (A–A''). Insets correspond to the regions of interest (ROI) marked in yellow shown with a 3D aspect. (C–C'') xz cross-sections of the scale at locations marked with gray lines in (A–A''). Scale bars (A–A'') 5 µm, (B–B'', C–C'', and insets) 1 µm. To better understand the mechanism behind F-actin reorganization, we investigated whether the actin-related proteins, Arp2/3 complex, is a potential factor regulating the dendritic growth of actin fibrils (Hudson and Cooley, 2002; Pollard, 2007; Rouiller et al., 2008). At around 52% development in P. eurimedes, Arp2/3 complex appear in a sparse punctate pattern while the F-actin bundles are still intact (Figure 4A–C and Figure 4—figure supplement 1). As F-actin bundles disintegrate and start to reorganize around 62% development, a relatively higher density of punctate Arp2/3 complex is seen in close association with the F-actin network (Figure 4A'–C' and Figure 4—figure supplement 2). At a later stage (~76% development), no distinctive punctate patterns can be discerned (Figure 4A''–C'' and Figure 4—figure supplement 3), which is expected given that the reorganization of F-actin is nearly complete by this stage (see Figure 3). However, the scales are more mature at this point with prominent ridges, and autofluorescence signal from the cuticle has shifted into the green wavelengths (Figure 4—figure supplement 4), complicating the interpretation of the Arp2/3 immunofluorescence signal. Figure 4 with 8 supplements see all Download asset Open asset Arp2/3 complex is involved in F-actin reorganization in pupal P. eurimedes wing scales. Dorsal forewing scales stained with AF-594 anti-Arp2 (yellow) and AF-647 phalloidin (magenta) acquired with 100× confocal microscope. Initially (~52% development), Arp2/3 complex appear as sparse punctate dots while the F-actin bundles are still intact. As F-actin bundles disintegrate and reorganize, a relatively higher density of punctate Arp2/3 signal is seen in close association with the reticulate F-actin network. Our oldest timepoint in P. eurimedes (~76% development) has a large amount of cuticle autofluorescence overlapping with the AF594 signal, but the punctate pattern can be still discerned. See also Figure 4—figure supplements 1–3. (B–B'') Closeup views of (A–A''). (C–C'') xz cross-sections of the scale at locations marked with gray lines in (A–A''). Yellow regions of interest (ROIs) correspond to those in (B–B''). Scale bars (A–A'') 5 µm, (B–B'' and C–C'') 2 µm. Pharmacological disruption of Arp2/3 using CK-666 In order to mechanistically test the putative role of Arp2/3 in reorganizing F-actin during honeycomb lattice formation, we carried out pharmacological disruption experiments in pupae of both Parides and Papilio species using CK-666, a known inhibitor of the Arp2/3 complex (Henson et al., 2015). Unfortunately, we were only able to obtain a small number of Parides pupae (N = 15), which were already somewhat mature when they arrived, and further, a third of the pupae were dead upon arrival (Supplementary file 1). Honeycomb lattices can be seen in scales from emerged males of both P. eurimedes and P. iphidamas pupae treated with CK-666 (Figure 4—figure supplement 5). However, the height of the honeycombs in scales from pupae treated on 52% development was on average lower than the control (Figure 4—figure supplement 5A and D). Furthermore, in P. eurimedes, the height of the honeycombs in scales from pupae injected at 57% development was higher on average than those treated at 52% development, but indistinguishable from any controls (Figure 4—figure supplement 5A). These results are reminiscent of a previous study utilizing cytochalasin-D to inhibit linear actin polymerization (Dinwiddie et al., 2014) that found that once the pupae were past a certain developmental stage, ridge formation is no longer disrupted by the inhibitor. However, despite this intriguing trend in our results being consistent with a hypothesized role for Arp2/3, we require data with robust sample sizes and younger time points (i.e., 28–48% development) for Parides spp. in future experiments to validate the role of Arp2/3. We also injected CK-666 into the wing pouches of the more readily available P. polytes pupae from 40 to 80% development to cover the possible timeframe during which Arp2/3 complex could be involved in F-actin reorganization. However, neither cuticular disks nor the dendritic reorganization of linear F-actin bundles are observed in P. polytes, whose adult scales have irregular crossribs without the deep lattice walls, that is, no honeycombs (Figure 4—figure supplement 6B and C). Shortly after ridge formation at 60% development (Figure 4—figure supplement 6D–D''), irregular crossribs are seen in between intact F-actin bundles (Figure 4—figure supplement 6F–F''). At 70% development, strong chitin autofluorescence signal is seen at the ridges (Figure 4—figure supplement 6H–I). Since F-actin reorganization is absent in this species, Arp2/3 inhibition did not disrupt ridge and crossrib formation in P. polytes as expected (Figure 4—figure supplement 7). Concurrent SEM imaging of pupal scales from the same samples shows that the formation of irregular crossribs is similarly not affected (Figure 4—figure supplement 8). Our results suggests the formation of cuticular disks and their subsequent extrusion by reorganizing F-actin is restricted to species with elaborate honeycomb lattice walls such as P. eurimedes. Honeycomb morphogenesis is conserved across Papilionidae We also assayed the development of pupal wing scales of several other papilionid species: P. arcas, P. nireus, and P. palinurus (Figure 5), Although we were unable to obtain pupae with clearly marked pupation dates for these three species, we observed similar early stages of honeycomb development as seen in P. eurimedes. In adult male P. arcas, green scales have well-developed honeycomb lattice walls that sit above single gyroid crystals (Figure 5A). During P. arcas pupal scale development, both solid and hollow (annular) cuticular disks can be observed in between intact linear actin bundles (Figure 5B–B' and C–C'). The same is observed in P. nireus, where the upper lamina of adult blue scales consists of an elaborate honeycomb lattice with smaller sized pores (Figure 5D–F). In P. palinurus adults, green forewing cover scales feature large concave depressions in between the ridges (Figure 5G). Phalloidin staining of pupal P. palinurus scales reveals reorganization of longitudinal F-actin bundles into a shallow concave network (concentric rings in cross-section) (Figure 5H and I and Figure 5—video 1), which closely correspond to the cuticular dimples seen in adult green cover scales. Figure 5 with 1 supplement see all Download asset Open asset Conservation of honeycomb lattice development in Papilionidae. Scanning electron microscopy (SEM) cross-sectional view of (A) adult green scales in P. arcas, (D) blue scales in P. nireus, and (G) green scales in P. palinurus. All pupal dorsal forewing scales are stained either with AF-555 wheat germ agglutinin (WGA) or FITC-WGA (yellow) showing chitin, and AF-647 phalloidin or TRITC-phalloidin (magenta) showing F-actin. (B, C) Maximum projected 3D structured illumination microscopy (3D-SIM) micrograph of green cover scales in pupal male P. arcas featuring planar cuticular disks similar in shape and arrangement to P. eurimedes. (B'–C') Maximum projected 3D-SIM micrograph of green cover scales of a different male P. arcas pupa showing irregular crossribs patterns. (E, F) Maximum projected 3D-SIM micrograph of blue cover scales of pupal P. nireus, similarly with crossribs and intact linear actin bundles. (H, I) Maximum projected 60× confocal micrographs of green cover scales of pupal P. palinurus. A concave network of F-actin underlies the cuticular dimples. At lower z, the actin rings (in cross-section) are smaller in size and show a foam-like appearance. (C–C', F, and I) xz cross-sections of the scales with region of interest (ROI) at locations marked with gray lines in (B–B'), (E), and (H), respectively. Scale bars (A, D, and G) 5 µm, (B–B', C–C', E, and F) 1 µm, (H, I) 5 µm. Discussion Swallowtail butterflies (Papilionidae) is the sister lineage to all other butterflies (Espeland et al., 2018) and the showcase family of lepidopterans as they exhibit some of the most diverse assortment of wing scale nanostructures (Ghiradella, 1985; Prum et al., 2006). Papilionid wing scales generally exhibit irregular crossribs, with some having underlying cuticular walls enclosing tubular pores, although the regularity and depth of the walls vary between species (Figure 1—figure supplement 1). These crossrib patterns are unlike the regular, rectilinear crossribs commonly seen in other lepidopteran families, for example, Nymphalidae (Figure 1—figure supplement 1R). In this study, we extended previous observations that parallel F-actin bundles configure the spacing and position of longitudinal ridges of wing scales (Day et al., 2019; Dinwiddie et al., 2014; Ghiradella, 1974; Pomerantz et al., 2020) to Papilionidae, further supporting the hypothesis that this mechanism is broadly conserved across lepidopterans. Our first novel finding is the appearance of irregular crossrib patterns in the plasma membrane between surface ridges, which seemed to serve as a template for chitin accretion into chitinous disks. This process is important as it marks the beginning of honeycomb lattice formation. Further, our results revealed that F-actin plays a predominant and previously unconsidered role in the morphogenesis of some papilionid butterfly wing scales. Once the longitudinal ridges have developed, F-actin bundles, which typically degenerate in nymphalid species (Day et al., 2019; Dinwiddie et al., 2014), subsequently reorganize into a reticulate network surrounding rows of cuticular disks on the scale surface. This actin network likely draws in plasma membrane underlying the cuticular disks into a porous honeycomb lattice. Similar nonlinear F-actin morphologies have been observed in other organisms. In diatoms, F-actin organizes into an interdigitating mesh-like porous network during development (Tesson and Hildebrand, 2010). This actin network defines frustule (cell walls) morphogenesis by providing a template for silica biomineralization at the meso- and microscales. In mammalian cells, transient ring-like F-actin structures are thought to drive autophagosome generation by serving as a scaffold for mitophagy initiation structures. 3D-SIM revealed F-actin partially associating with mitochondria in the form of curved sheet (Hsieh and Yang, 2019), akin to the F-actin structures seen in this study. The schematic in Figure 6 illustrates our proposed formation of honeycomb lattices in some papilionid wing scales. During early stages of scale formation, F-actin bundles prefigure the loci of future ridge formation in between adjacent actin bundles (Dinwiddie et al., 2014). Plasma membrane then forms a scaffold resembling the irregular crossrib pattern. The cuticle accretes into roughly circular disks delineated by the scaffold. Reorganization of F-actin around the chitinous disks draws the plasma membrane along with the overlying cuticle inward, molding it into the lengthened cuticular walls of the honeycomb lattice. Figure 6 Download asset Open asset A schematic illustrating the proposed morphogenesis of honeycomb lattices in papilionid wing scales. During early stages, F-actin bundles prefigure the loci where ridges form in between adjacent actin bundles. Next the plasma membrane (black lines) forms a scaffold where cuticle (green lines and structures) accumulates into the spaces and forms irregular disk-like structures. Reorganization of F-actin around the disks subsequently extrudes the plasma membrane along with the deposited cuticle, forming the honeycomb lattice walls. In our attempt to unravel the molecular players in actin reorganization, we used CK-666 to inhibit the role of Arp2/3 complex in actin branching (Henson et al., 2015). However, CK-666 did not quite disrupt the formation of honeycombs even when administered across multiple timepoints. A variety of reasons could potentially explain this. While 100 uM of CK-666 has been shown to work in other animal models (Henson et al., 2015), it might still not be a sufficient titer for papilionid butterfly pupal scales. Unfortunately, due to logistical difficulties in obtaining and rearing Parides spp., we were unable to perform a thorough optimization using CK-666. Furthermore, the Parides pupae were likely too mature at the time of CK-666 treatment, when the F-actin network has already been established (52% development). In a recent study (Sakamoto et al., 2018), CK-666 did not disrupt the formation of cortical actin meshwork in Sertoli cells of mouse seminiferous tubules as they were found to be regulated by formins rather than Arp2/3. This suggests that other molecular players, including formins, could be involved in wing scale honeycomb morphogenesis and need testing. We note that the irregular crossrib patterns are in place while the ridges are still growing (Figure 2A'–C'). These crossribs appear to constrain and delineate the accumulation of cuticle into planar disks in between the ridges. A close examination of SEM images (Figure 1B and Figure 1—figure supplement 1) reveals that the endpoints of crossribs at the ridge interface are often connected to microribs present on the ridges. Given their relatively small size and pitch, we are unable to resolve the development of microribs here, which likely requires imaging techniques with even higher resolution such as photo-activated localization microscopy and stochastic optical reconstruction microscopy. However, both the periodic stripes (microribs) and quasi-periodic spots (crossribs) are reminiscent of Turing patterns (Meinhardt, 1982; Turing, 1952), that is, implying that an activator-inhibitor type mechanism could be involved in their formation. Alternatively, the crossrib pattern could be produced as a result of spontaneous processes like Ostwald ripening, phase separation, and/or biomechanical forces determined by the mechanical properties of the membranes, leading to stable, quasi-periodic, foam-like perforations on the plasma membrane in between ridges (Dinwiddie et al., 2014; Elson et al., 2010; McDougal et al., 2021; Rosetti et al., 2017; Voorhees, 1985; Wilts et al., 2019). Overall, the presence of chitinous disks followed by F-actin reorganization appears to be specific to species with extended honeycomb lattice walls, for example, P. eurimedes, P. arcas, and P. nireus (Figure 1—figure supplement 1A and E). Such reorganized actin structures are not observed in species with shallow irregular crossribs, such as P. polytes (Figure 4—figure supplement 6). On the other hand, several Papilio species (e.g., palinurus, blumei, karna) possess multilayered cover scales (Ghiradella, 1974; Prum et al., 2006; Trzeciak et al., 2012) with widely spaced and reduced (in both height and number) ridges. These scales have no apparent crossribs, trabeculae, or honeycombs. Instead, they have a characteristic inter-ridge array of concave depressions and underlying perforated lamellae in the scale interior (Figure 1—figure supplement 1L and M). Ba" @default.
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• W4387141282 title "eLife assessment: Hierarchical morphogenesis of swallowtail butterfly wing scale nanostructures" @default.
• W4387141282 doi "https://doi.org/10.7554/elife.89082.3.sa0" @default.
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