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• W4387157981 abstract "AbstractThe objective of this study is to establish strategies to uniformly proliferate cells in a three-dimensional non-woven polyethylene terephthalate (PET)/ethylene vinyl alcohol (EVOH) scaffold by simple adjustments in seeding and culture methods and the scaffold design. The combined dynamic and static seeding (intermittent agitations at 300 rpm with 1 hr intervals) resulted in the highest seeding efficiency (71%) comparing to the static and continuous agitating seeding methods. Cell-attached scaffolds were cultivated under different conditions. The stirring culture permitted cells to proliferate to a significantly greater extent than the static or agitating cultures, although faster cell proliferation in the outer region of scaffold was observed. Next, based on this observation, scaffolds were opened with holes to alleviate the cell aggregation. Effect of hole size and number of scaffolds on the distribution of cells proliferated in the scaffold was evaluated. Two of 1-mm holes showed to be an optimal adjustment to allow cells to proliferate in a homogeneous manner. After 14 days culture, both of the holes were filled by cells proliferated with a 4-fold increase in the cell number. The cell viability in the scaffolds was also high upon evaluated the live/dead and 3[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) staining examinations. Different cell types of 3T3L1, C3H/10T1/2, and KUM6 cells showed the similar behavior of cell proliferation and distribution in the scaffold, indicating the applicability of the established procedure. It is concluded that the non-woven PET/EVOH scaffold serves as a potential cell culture substrate for an efficient cell proliferation.Keywords: 3D scaffoldnon-woven fabricmacroholeuniform cell proliferationPET/EVOHDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. FundingThe author(s) reported there is no funding associated with the work featured in this article." @default.
• W4387157981 created "2023-09-30" @default.
• W4387157981 creator A5055033211 @default.
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• W4387157981 date "2023-09-29" @default.
• W4387157981 modified "2023-10-11" @default.
• W4387157981 title "Cell culture design for homogeneous proliferation of cells in three-dimensional non-woven polymer scaffolds" @default.
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• W4387157981 doi "https://doi.org/10.1080/09205063.2023.2265623" @default.
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