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- W4387162521 abstract "Cell-free protein synthesis provides a flexible platform for the production of difficult-to-express proteins, because maintaining cell viability is unnecessary. The antimicrobial proteins known as bacteriocins have great potential for development as antibiotic alternatives. Here, we describe detailed protocols for producing and characterizing colicins-antimicrobial proteins that are produced by Escherichia coli hosts and inactivate nonhost E. coli strains. Active colicins can be produced with lysates containing molecular chaperones and coproduction of immunity proteins in cell-free protein synthesis reactions." @default.
- W4387162521 created "2023-09-30" @default.
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- W4387162521 date "2023-09-30" @default.
- W4387162521 modified "2023-10-17" @default.
- W4387162521 title "Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production" @default.
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- W4387162521 doi "https://doi.org/10.1007/978-1-0716-3469-1_1" @default.
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