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• W4387163911 abstract "L-Leucine is a high-value amino acid with promising applications in the medicine and feed industries. However, the complex metabolic network and intracellular redox imbalance in fermentative microbes limit their efficient biosynthesis of L-leucine.In this study, we applied rational metabolic engineering and a dynamic regulation strategy to construct a plasmid-free, non-auxotrophic Escherichia coli strain that overproduces L-leucine. First, the L-leucine biosynthesis pathway was strengthened through multi-step rational metabolic engineering. Then, a cooperative cofactor utilization strategy was designed to ensure redox balance for L-leucine production. Finally, to further improve the L-leucine yield, a toggle switch for dynamically controlling sucAB expression was applied to accurately regulate the tricarboxylic acid cycle and the carbon flux toward L-leucine biosynthesis. Strain LEU27 produced up to 55 g/L of L-leucine, with a yield of 0.23 g/g glucose.The combination of strategies can be applied to the development of microbial platforms that produce L-leucine and its derivatives." @default.
• W4387163911 created "2023-09-30" @default.
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• W4387163911 date "2023-09-29" @default.
• W4387163911 modified "2023-10-09" @default.
• W4387163911 title "Construction of a plasmid-free l-leucine overproducing Escherichia coli strain through reprogramming of the metabolic flux" @default.
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• W4387163911 doi "https://doi.org/10.1186/s13068-023-02397-x" @default.
• W4387163911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37775757" @default.
• W4387163911 hasPublicationYear "2023" @default.
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