Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387164549> ?p ?o ?g. }
- W4387164549 abstract "Autophagy induction involves extensive molecular and membrane reorganization. Despite substantial progress, the mechanism underlying autophagy initiation remains poorly understood. Here, we used quantitative photoactivated localization microscopy with single-molecule sensitivity to analyze the nanoscopic distribution of endogenous ULK1, the kinase that triggers autophagy. Under amino acid starvation, ULK1 formed large clusters containing up to 161 molecules at the endoplasmic reticulum. Cross-correlation analysis revealed that ULK1 clusters engaging in autophagosome formation require 30 or more molecules. The ULK1 structures with more than the threshold number contained varying levels of Atg13, Atg14, Atg16, LC3B, GEC1, and WIPI2. We found that ULK1 activity is dispensable for the initial clustering of ULK1, but necessary for the subsequent expansion of the clusters, which involves interaction with Atg14, Atg16, and LC3B and relies on Vps34 activity. This quantitative analysis at the single-molecule level has provided unprecedented insights into the behavior of ULK1 during autophagy initiation." @default.
- W4387164549 created "2023-09-30" @default.
- W4387164549 creator A5012086852 @default.
- W4387164549 creator A5014249671 @default.
- W4387164549 creator A5039043368 @default.
- W4387164549 creator A5046688257 @default.
- W4387164549 creator A5052448663 @default.
- W4387164549 creator A5061332658 @default.
- W4387164549 creator A5072386159 @default.
- W4387164549 date "2023-09-29" @default.
- W4387164549 modified "2023-10-15" @default.
- W4387164549 title "ULK1 forms distinct oligomeric states and nanoscopic structures during autophagy initiation" @default.
- W4387164549 cites W1523693806 @default.
- W4387164549 cites W1601216591 @default.
- W4387164549 cites W1624868929 @default.
- W4387164549 cites W1918384196 @default.
- W4387164549 cites W1965661521 @default.
- W4387164549 cites W1973163678 @default.
- W4387164549 cites W1974279922 @default.
- W4387164549 cites W1987900501 @default.
- W4387164549 cites W1988554417 @default.
- W4387164549 cites W1996068251 @default.
- W4387164549 cites W2004032495 @default.
- W4387164549 cites W2004851762 @default.
- W4387164549 cites W2036568535 @default.
- W4387164549 cites W2043891370 @default.
- W4387164549 cites W2048030072 @default.
- W4387164549 cites W2052618262 @default.
- W4387164549 cites W2054455698 @default.
- W4387164549 cites W2057764483 @default.
- W4387164549 cites W2062552264 @default.
- W4387164549 cites W2063096840 @default.
- W4387164549 cites W2064815984 @default.
- W4387164549 cites W2064853491 @default.
- W4387164549 cites W2068391177 @default.
- W4387164549 cites W2073576660 @default.
- W4387164549 cites W2076570319 @default.
- W4387164549 cites W2077409117 @default.
- W4387164549 cites W2081057817 @default.
- W4387164549 cites W2086096948 @default.
- W4387164549 cites W2094241924 @default.
- W4387164549 cites W2096261947 @default.
- W4387164549 cites W2109034822 @default.
- W4387164549 cites W2113432343 @default.
- W4387164549 cites W2114847797 @default.
- W4387164549 cites W2132763961 @default.
- W4387164549 cites W2136485408 @default.
- W4387164549 cites W2138617109 @default.
- W4387164549 cites W2139868016 @default.
- W4387164549 cites W2141541555 @default.
- W4387164549 cites W2145157070 @default.
- W4387164549 cites W2154516787 @default.
- W4387164549 cites W2155810842 @default.
- W4387164549 cites W2156144699 @default.
- W4387164549 cites W2161803468 @default.
- W4387164549 cites W2193239553 @default.
- W4387164549 cites W2231357824 @default.
- W4387164549 cites W2231569015 @default.
- W4387164549 cites W2330972395 @default.
- W4387164549 cites W2397093333 @default.
- W4387164549 cites W2398577671 @default.
- W4387164549 cites W2514327877 @default.
- W4387164549 cites W2545595629 @default.
- W4387164549 cites W2556834045 @default.
- W4387164549 cites W2567008309 @default.
- W4387164549 cites W2600590737 @default.
- W4387164549 cites W2617561678 @default.
- W4387164549 cites W2744062609 @default.
- W4387164549 cites W2756341799 @default.
- W4387164549 cites W2782609861 @default.
- W4387164549 cites W2894519757 @default.
- W4387164549 cites W2912386236 @default.
- W4387164549 cites W2950208982 @default.
- W4387164549 cites W2964400130 @default.
- W4387164549 cites W2977251762 @default.
- W4387164549 cites W3016296705 @default.
- W4387164549 cites W3094582012 @default.
- W4387164549 cites W3097055298 @default.
- W4387164549 cites W3214143288 @default.
- W4387164549 cites W4378172260 @default.
- W4387164549 cites W90071970 @default.
- W4387164549 doi "https://doi.org/10.1126/sciadv.adh4094" @default.
- W4387164549 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37774021" @default.
- W4387164549 hasPublicationYear "2023" @default.
- W4387164549 type Work @default.
- W4387164549 citedByCount "0" @default.
- W4387164549 crossrefType "journal-article" @default.
- W4387164549 hasAuthorship W4387164549A5012086852 @default.
- W4387164549 hasAuthorship W4387164549A5014249671 @default.
- W4387164549 hasAuthorship W4387164549A5039043368 @default.
- W4387164549 hasAuthorship W4387164549A5046688257 @default.
- W4387164549 hasAuthorship W4387164549A5052448663 @default.
- W4387164549 hasAuthorship W4387164549A5061332658 @default.
- W4387164549 hasAuthorship W4387164549A5072386159 @default.
- W4387164549 hasBestOaLocation W43871645491 @default.
- W4387164549 hasConcept C12554922 @default.
- W4387164549 hasConcept C158617107 @default.
- W4387164549 hasConcept C168296220 @default.
- W4387164549 hasConcept C171250308 @default.
- W4387164549 hasConcept C171896709 @default.