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• W4387165885 abstract "The high cost associated with polyhydroxybutyrate (PHB) production demands immediate attention to advance its commercial viability. Herein, we innovatively construct highly efficient production of PHB from low-cost lignocellulosic biomass (LCB) using an open one-pot fermentation by the alkali-halophilic Halomonas alkalicola M2 developed in our previous work. We first develop alkaline pretreatment of LCB to achieve open one-pot fermentation and efficient conversion. Multi-objective optimization of fermentation conditions produces 103.421 mg/L of PHB with a content of 14.437 % and effectively promotes saccharification with cellulose and xylan enzymatic efficiencies of 82.948 % and 73.710 %, respectively. We then develop an open dual one-pot fermentation process, involving the conversion of lignin- and lignin-derived compounds-enriched slurry in the first pot and subsequent polysaccharide conversion in the second pot, to achieve efficient whole LCB components conversion, yielding a record 9.57 g/L of PHB. More importantly, we extend an open dual one-pot-fed-batch fermentation, producing 0.80 g/L of PHB with 27.69 % content in the first pot and 20.08 g/L of PHB with 61.86 % content in the second pot, showing significantly higher than many reported strains. Overall, this work demonstrates the effectiveness of utilizing cheap LCB in a newly designed fermentation process to produce PHB efficiently, representing a significant advancement in this field." @default.
• W4387165885 created "2023-09-30" @default.
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• W4387165885 date "2023-11-01" @default.
• W4387165885 modified "2023-10-09" @default.
• W4387165885 title "Highly efficient production of polyhydroxybutyrate using an open dual one-pot fermentation by Halomonas alkalicola M2" @default.
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• W4387165885 doi "https://doi.org/10.1016/j.cej.2023.146327" @default.
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