FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387168049> ?p ?o ?g. }

• W4387168049 abstract "ABSTRACT Objective Bmp6 knockout (KO) mice progressively accumulate a significant amount of iron in their liver as they age due to a defect in hepcidin (Hamp) expression and an upregulation of the iron exporter ferroportin (Fpn). In this study, we conducted a comprehensive investigation of the hepatic iron overload phenotype, with specific emphasis on the cellular and subcellular localization of Fpn in Bmp6 KO mice. Materials and Methods Livers obtained from Bmp6 knockout (KO) mice at different developmental stages were utilized for the quantification of iron content, investigation of iron distribution, histological analysis, histoimmunofluorescence assays performed on paraffin-embedded sections, confocal microscopy examinations, subcellular membrane fractionation, and western blot analysis. Results In Bmp6 KO livers, iron overload increased with age and was not homogeneous, with certain hepatic lobes and specific areas in liver sections showing more pronounced iron accumulation. In young mice, iron accumulated mostly in the centrilobular zone where low Fpn expression was observed. Fpn was strongly detected in periportal Kupffer cells and at the apical membrane of periportal hepatocytes lining the sinusoidal capillaries. The zonal distribution of iron tended to disappear with age in strongly iron-overloaded areas, with the appearance of large cellular aggregates strongly positive for Fpn, iron, and ceroid/lipofuscin. At the subcellular level, hepatic Fpn seemed to concentrate in specific cell surface compartments and was enriched in a lipid raft fraction. Conclusions Unregulated expression of Fpn on the cell surface of periportal macrophages and hepatocytes results in centrilobular iron overload within hepatocytes. In areas of pronounced iron overload, Fpn expression is present in lipogranulomas, identified as aggregations of macrophages accumulating hemosiderin and ceroid/lipofuscin pigments. These lesions likely form due to the phagocytosis of sideronecrotic/ferroptotic hepatocytes by macrophages. In contrast to the duodenal form of Fpn, both splenic and hepatic Fpn demonstrated robust enrichment within lipid rafts. The observed variations in the subcellular localization of Fpn could play a significant role in influencing the transporter’s iron transport activity and/or its regulation by hepcidin." @default.
• W4387168049 created "2023-09-30" @default.
• W4387168049 creator A5008663064 @default.
• W4387168049 creator A5037719295 @default.
• W4387168049 creator A5042002053 @default.
• W4387168049 creator A5042112338 @default.
• W4387168049 creator A5048550199 @default.
• W4387168049 creator A5075432044 @default.
• W4387168049 creator A5088274861 @default.
• W4387168049 date "2023-09-29" @default.
• W4387168049 modified "2023-10-16" @default.
• W4387168049 title "NEW INSIGHTS INTO THE HEPATIC IRON PHENOTYPE OF BMP6 KNOCKOUT MICE" @default.
• W4387168049 cites W130498243 @default.
• W4387168049 cites W1579469490 @default.
• W4387168049 cites W1618001767 @default.
• W4387168049 cites W1965282264 @default.
• W4387168049 cites W1968323673 @default.
• W4387168049 cites W1977767277 @default.
• W4387168049 cites W1989831120 @default.
• W4387168049 cites W1990330893 @default.
• W4387168049 cites W1994834562 @default.
• W4387168049 cites W2013232839 @default.
• W4387168049 cites W2018391935 @default.
• W4387168049 cites W2049537480 @default.
• W4387168049 cites W2055631866 @default.
• W4387168049 cites W2063162117 @default.
• W4387168049 cites W2074000647 @default.
• W4387168049 cites W2074759995 @default.
• W4387168049 cites W2078643425 @default.
• W4387168049 cites W2087744489 @default.
• W4387168049 cites W2095066403 @default.
• W4387168049 cites W2101071630 @default.
• W4387168049 cites W2101748158 @default.
• W4387168049 cites W2117446787 @default.
• W4387168049 cites W2136558879 @default.
• W4387168049 cites W2142833570 @default.
• W4387168049 cites W2145306168 @default.
• W4387168049 cites W2150887163 @default.
• W4387168049 cites W2154439794 @default.
• W4387168049 cites W2159430841 @default.
• W4387168049 cites W2563382573 @default.
• W4387168049 cites W2911912494 @default.
• W4387168049 cites W4206972726 @default.
• W4387168049 cites W4295902096 @default.
• W4387168049 cites W4386439125 @default.
• W4387168049 doi "https://doi.org/10.1101/2023.09.28.559941" @default.
• W4387168049 hasPublicationYear "2023" @default.
• W4387168049 type Work @default.
• W4387168049 citedByCount "0" @default.
• W4387168049 crossrefType "posted-content" @default.
• W4387168049 hasAuthorship W4387168049A5008663064 @default.
• W4387168049 hasAuthorship W4387168049A5037719295 @default.
• W4387168049 hasAuthorship W4387168049A5042002053 @default.
• W4387168049 hasAuthorship W4387168049A5042112338 @default.
• W4387168049 hasAuthorship W4387168049A5048550199 @default.
• W4387168049 hasAuthorship W4387168049A5075432044 @default.
• W4387168049 hasAuthorship W4387168049A5088274861 @default.
• W4387168049 hasBestOaLocation W43871680491 @default.
• W4387168049 hasConcept C104317684 @default.
• W4387168049 hasConcept C126322002 @default.
• W4387168049 hasConcept C127716648 @default.
• W4387168049 hasConcept C142724271 @default.
• W4387168049 hasConcept C170493617 @default.
• W4387168049 hasConcept C182704531 @default.
• W4387168049 hasConcept C185592680 @default.
• W4387168049 hasConcept C186198217 @default.
• W4387168049 hasConcept C2776415932 @default.
• W4387168049 hasConcept C2776710925 @default.
• W4387168049 hasConcept C2776768029 @default.
• W4387168049 hasConcept C2778248108 @default.
• W4387168049 hasConcept C2780883591 @default.
• W4387168049 hasConcept C55493867 @default.
• W4387168049 hasConcept C71924100 @default.
• W4387168049 hasConcept C86803240 @default.
• W4387168049 hasConcept C95444343 @default.
• W4387168049 hasConceptScore W4387168049C104317684 @default.
• W4387168049 hasConceptScore W4387168049C126322002 @default.
• W4387168049 hasConceptScore W4387168049C127716648 @default.
• W4387168049 hasConceptScore W4387168049C142724271 @default.
• W4387168049 hasConceptScore W4387168049C170493617 @default.
• W4387168049 hasConceptScore W4387168049C182704531 @default.
• W4387168049 hasConceptScore W4387168049C185592680 @default.
• W4387168049 hasConceptScore W4387168049C186198217 @default.
• W4387168049 hasConceptScore W4387168049C2776415932 @default.
• W4387168049 hasConceptScore W4387168049C2776710925 @default.
• W4387168049 hasConceptScore W4387168049C2776768029 @default.
• W4387168049 hasConceptScore W4387168049C2778248108 @default.
• W4387168049 hasConceptScore W4387168049C2780883591 @default.
• W4387168049 hasConceptScore W4387168049C55493867 @default.
• W4387168049 hasConceptScore W4387168049C71924100 @default.
• W4387168049 hasConceptScore W4387168049C86803240 @default.
• W4387168049 hasConceptScore W4387168049C95444343 @default.
• W4387168049 hasLocation W43871680491 @default.
• W4387168049 hasOpenAccess W4387168049 @default.
• W4387168049 hasPrimaryLocation W43871680491 @default.
• W4387168049 hasRelatedWork W1482134942 @default.
• W4387168049 hasRelatedWork W1980042430 @default.
• W4387168049 hasRelatedWork W2055536483 @default.
• W4387168049 hasRelatedWork W2734249968 @default.
• W4387168049 hasRelatedWork W2769664694 @default.

• next