Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387169310> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W4387169310 endingPage "s80" @default.
- W4387169310 startingPage "s80" @default.
- W4387169310 abstract "Background: Multidrug-resistant organisms (MDROs) are increasingly implicated in nosocomial outbreaks worldwide. We evaluated whole-genome sequencing (WGS) as an adjunctive epidemiological tool to identify infection clusters and MDRO transmission in the healthcare setting. Methods: Clinical isolates of carbapenem-resistant Enterobacterales (CRE) from July 1, 2021, to June 30, 2022, underwent Illumina WGS. Assembled genomes were taxonomically classified with GTDB-Tk software and were typed using multilocus sequence typing (MLST). Average nucleotide identity (ANI) was calculated between genomes. Numbers of differences among core single-nucleotide polymorphisms (SNPs) were calculated for pairs within taxonomic groups, and the data were evaluated in the context of patient dates and locations of care obtained from the electronic medical record. Results: In total, 39 CRE isolates underwent WGS (Fig. 1). Klebsiella pneumoniae represented the largest number of isolates (n = 18). Using MLST, 2 distinct groups of K. pneumoniae were identified (ST307 and ST258) with 5 and 4 isolates, respectively (Fig. 2). Within ST307, SNP differences ranged between 8 and 115. 3 isolates (CRE8, CRE10, and CRE12) were collected within 4 weeks of each other and had ≤26 pairwise SNP differences. Notably, CRE8 and CRE10 were located on the same unit at the same time and used the same MRI scanner on the same day. CRE35 had >95 SNP differences and was admitted 8 months prior to others in ST307 but had surgery in the same OR as CRE8. Within ST258, pairwise comparison of samples revealed 139–588 SNP differences. CRE21, CRE31, and CRE33 had SNP differences of ≤150. These patients were in the same hospital room (CRE33 and CRE21) and unit (CRE31 and CRE33), but they did not overlap temporally. CRE37 had >580 SNP differences, with no overlap in hospitalization dates or locations with other patients. Conclusions: Two closely related K. pneumoniae isolate populations were identified using WGS. Strong temporal and spatial commonalities were identified among isolates with few SNP differences. Isolate pairs with intermediate SNP differences shared spatial commonalities, suggesting possible indirect transmission between patients. No common exposures were identified for pairs with large numbers of SNP differences. WGS is an evolving tool to detect outbreak clonal populations of MDRO not identified through traditional epidemiologic techniques. WGS can provide insight into transmission patterns and the role of environmental contamination in propagating these nosocomial infections. More studies are needed to define the role and clinical significance of isolates with intermediate SNP differences in transmission of these pathogens between hosts and the healthcare environment. Disclosures: None" @default.
- W4387169310 created "2023-09-30" @default.
- W4387169310 creator A5016456474 @default.
- W4387169310 creator A5017657671 @default.
- W4387169310 creator A5025176676 @default.
- W4387169310 creator A5029299597 @default.
- W4387169310 creator A5037581229 @default.
- W4387169310 creator A5055075306 @default.
- W4387169310 creator A5065770157 @default.
- W4387169310 creator A5073753827 @default.
- W4387169310 creator A5075486537 @default.
- W4387169310 creator A5088863009 @default.
- W4387169310 date "2023-06-01" @default.
- W4387169310 modified "2023-09-30" @default.
- W4387169310 title "Whole-genome sequencing of carbapenem-resistant Enterobacterales isolates and evaluation of hospital-acquired infections" @default.
- W4387169310 doi "https://doi.org/10.1017/ash.2023.335" @default.
- W4387169310 hasPublicationYear "2023" @default.
- W4387169310 type Work @default.
- W4387169310 citedByCount "0" @default.
- W4387169310 crossrefType "journal-article" @default.
- W4387169310 hasAuthorship W4387169310A5016456474 @default.
- W4387169310 hasAuthorship W4387169310A5017657671 @default.
- W4387169310 hasAuthorship W4387169310A5025176676 @default.
- W4387169310 hasAuthorship W4387169310A5029299597 @default.
- W4387169310 hasAuthorship W4387169310A5037581229 @default.
- W4387169310 hasAuthorship W4387169310A5055075306 @default.
- W4387169310 hasAuthorship W4387169310A5065770157 @default.
- W4387169310 hasAuthorship W4387169310A5073753827 @default.
- W4387169310 hasAuthorship W4387169310A5075486537 @default.
- W4387169310 hasAuthorship W4387169310A5088863009 @default.
- W4387169310 hasBestOaLocation W43871693101 @default.
- W4387169310 hasConcept C104317684 @default.
- W4387169310 hasConcept C135763542 @default.
- W4387169310 hasConcept C139275648 @default.
- W4387169310 hasConcept C141231307 @default.
- W4387169310 hasConcept C151730666 @default.
- W4387169310 hasConcept C153209595 @default.
- W4387169310 hasConcept C193252679 @default.
- W4387169310 hasConcept C24432333 @default.
- W4387169310 hasConcept C2777058267 @default.
- W4387169310 hasConcept C2779343474 @default.
- W4387169310 hasConcept C2781209916 @default.
- W4387169310 hasConcept C54355233 @default.
- W4387169310 hasConcept C547475151 @default.
- W4387169310 hasConcept C77434933 @default.
- W4387169310 hasConcept C86803240 @default.
- W4387169310 hasConceptScore W4387169310C104317684 @default.
- W4387169310 hasConceptScore W4387169310C135763542 @default.
- W4387169310 hasConceptScore W4387169310C139275648 @default.
- W4387169310 hasConceptScore W4387169310C141231307 @default.
- W4387169310 hasConceptScore W4387169310C151730666 @default.
- W4387169310 hasConceptScore W4387169310C153209595 @default.
- W4387169310 hasConceptScore W4387169310C193252679 @default.
- W4387169310 hasConceptScore W4387169310C24432333 @default.
- W4387169310 hasConceptScore W4387169310C2777058267 @default.
- W4387169310 hasConceptScore W4387169310C2779343474 @default.
- W4387169310 hasConceptScore W4387169310C2781209916 @default.
- W4387169310 hasConceptScore W4387169310C54355233 @default.
- W4387169310 hasConceptScore W4387169310C547475151 @default.
- W4387169310 hasConceptScore W4387169310C77434933 @default.
- W4387169310 hasConceptScore W4387169310C86803240 @default.
- W4387169310 hasIssue "S2" @default.
- W4387169310 hasLocation W43871693101 @default.
- W4387169310 hasOpenAccess W4387169310 @default.
- W4387169310 hasPrimaryLocation W43871693101 @default.
- W4387169310 hasRelatedWork W1923230355 @default.
- W4387169310 hasRelatedWork W1995519181 @default.
- W4387169310 hasRelatedWork W2026728845 @default.
- W4387169310 hasRelatedWork W2082710782 @default.
- W4387169310 hasRelatedWork W2144462209 @default.
- W4387169310 hasRelatedWork W2293346226 @default.
- W4387169310 hasRelatedWork W2740216169 @default.
- W4387169310 hasRelatedWork W3211970273 @default.
- W4387169310 hasRelatedWork W4284705443 @default.
- W4387169310 hasRelatedWork W2739666146 @default.
- W4387169310 hasVolume "3" @default.
- W4387169310 isParatext "false" @default.
- W4387169310 isRetracted "false" @default.
- W4387169310 workType "article" @default.