Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387242024> ?p ?o ?g. }
- W4387242024 abstract "Sequences that form DNA secondary structures, such as G-quadruplexes (G4s) and intercalated-Motifs (iMs), are abundant in the human genome and play various physiological roles. However, they can also interfere with replication and threaten genome stability. Multiple lines of evidence suggest G4s inhibit replication, but the underlying mechanism remains unclear. Moreover, evidence of how iMs affect the replisome is lacking. Here, we reconstitute replication of physiologically derived structure-forming sequences to find that a single G4 or iM arrest DNA replication. Direct single-molecule structure detection within solid-state nanopores reveals structures form as a consequence of replication. Combined genetic and biophysical characterisation establishes that structure stability and probability of structure formation are key determinants of replisome arrest. Mechanistically, replication arrest is caused by impaired synthesis, resulting in helicase-polymerase uncoupling. Significantly, iMs also induce breakage of nascent DNA. Finally, stalled forks are only rescued by a specialised helicase, Pif1, but not Rrm3, Sgs1, Chl1 or Hrq1. Altogether, we provide a mechanism for quadruplex structure formation and resolution during replication and highlight G4s and iMs as endogenous sources of replication stress." @default.
- W4387242024 created "2023-10-03" @default.
- W4387242024 creator A5004417012 @default.
- W4387242024 creator A5007096853 @default.
- W4387242024 creator A5008473311 @default.
- W4387242024 creator A5035077989 @default.
- W4387242024 creator A5041237515 @default.
- W4387242024 creator A5051282109 @default.
- W4387242024 creator A5053044199 @default.
- W4387242024 creator A5060736255 @default.
- W4387242024 creator A5076346374 @default.
- W4387242024 creator A5077846914 @default.
- W4387242024 creator A5079239498 @default.
- W4387242024 creator A5089523004 @default.
- W4387242024 date "2023-10-02" @default.
- W4387242024 modified "2023-10-17" @default.
- W4387242024 title "Replication‐induced DNA secondary structures drive fork uncoupling and breakage" @default.
- W4387242024 cites W1450574606 @default.
- W4387242024 cites W1534291862 @default.
- W4387242024 cites W1960108177 @default.
- W4387242024 cites W1965423318 @default.
- W4387242024 cites W1965853538 @default.
- W4387242024 cites W1984693763 @default.
- W4387242024 cites W1986071629 @default.
- W4387242024 cites W1991754576 @default.
- W4387242024 cites W1992223960 @default.
- W4387242024 cites W1994474261 @default.
- W4387242024 cites W1997528341 @default.
- W4387242024 cites W1997963513 @default.
- W4387242024 cites W1998590756 @default.
- W4387242024 cites W2008182786 @default.
- W4387242024 cites W2008871447 @default.
- W4387242024 cites W2010595848 @default.
- W4387242024 cites W2027738669 @default.
- W4387242024 cites W2032640722 @default.
- W4387242024 cites W2037291224 @default.
- W4387242024 cites W2040860750 @default.
- W4387242024 cites W2043720815 @default.
- W4387242024 cites W2045840287 @default.
- W4387242024 cites W2048170340 @default.
- W4387242024 cites W2048897962 @default.
- W4387242024 cites W2050913592 @default.
- W4387242024 cites W2052442164 @default.
- W4387242024 cites W2054378721 @default.
- W4387242024 cites W2054825565 @default.
- W4387242024 cites W2056375209 @default.
- W4387242024 cites W2061494556 @default.
- W4387242024 cites W2064310741 @default.
- W4387242024 cites W2064809185 @default.
- W4387242024 cites W2065652369 @default.
- W4387242024 cites W2067613039 @default.
- W4387242024 cites W2073722866 @default.
- W4387242024 cites W2077891224 @default.
- W4387242024 cites W2080311192 @default.
- W4387242024 cites W2080862728 @default.
- W4387242024 cites W2081446791 @default.
- W4387242024 cites W2082286833 @default.
- W4387242024 cites W2083158919 @default.
- W4387242024 cites W2085365618 @default.
- W4387242024 cites W2085617522 @default.
- W4387242024 cites W2093320042 @default.
- W4387242024 cites W2104426389 @default.
- W4387242024 cites W2109887615 @default.
- W4387242024 cites W2126007346 @default.
- W4387242024 cites W2127213026 @default.
- W4387242024 cites W2128322002 @default.
- W4387242024 cites W2132070326 @default.
- W4387242024 cites W2138222569 @default.
- W4387242024 cites W2155773930 @default.
- W4387242024 cites W2158737337 @default.
- W4387242024 cites W2164144387 @default.
- W4387242024 cites W2169745877 @default.
- W4387242024 cites W2179797565 @default.
- W4387242024 cites W2192331052 @default.
- W4387242024 cites W2327094479 @default.
- W4387242024 cites W2329887558 @default.
- W4387242024 cites W2411316500 @default.
- W4387242024 cites W2462761630 @default.
- W4387242024 cites W2473838984 @default.
- W4387242024 cites W2512389053 @default.
- W4387242024 cites W2520749639 @default.
- W4387242024 cites W2548136543 @default.
- W4387242024 cites W2562393208 @default.
- W4387242024 cites W2568780937 @default.
- W4387242024 cites W2586727998 @default.
- W4387242024 cites W2590204906 @default.
- W4387242024 cites W2598475451 @default.
- W4387242024 cites W2604825272 @default.
- W4387242024 cites W2620256087 @default.
- W4387242024 cites W2621220151 @default.
- W4387242024 cites W2742893306 @default.
- W4387242024 cites W2749895930 @default.
- W4387242024 cites W2750812953 @default.
- W4387242024 cites W2774908616 @default.
- W4387242024 cites W2800768216 @default.
- W4387242024 cites W2885236691 @default.
- W4387242024 cites W2886241640 @default.
- W4387242024 cites W2893373509 @default.
- W4387242024 cites W2894864443 @default.
- W4387242024 cites W2900339116 @default.