Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387302408> ?p ?o ?g. }
- W4387302408 endingPage "14854" @default.
- W4387302408 startingPage "14854" @default.
- W4387302408 abstract "Protein kinases are key regulators of cell signaling and have been important therapeutic targets for three decades. ATP-competitive drugs directly inhibit the activity of kinases but these enzymes work as part of complex protein networks in which protein-protein interactions (often referred to as kinase docking) may govern a more complex activation pattern. Kinase docking is indispensable for many signaling disease-relevant Ser/Thr kinases and it is mediated by a dedicated surface groove on the kinase domain which is distinct from the substrate-binding pocket. Thus, interfering with kinase docking provides an alternative strategy to control kinases. We describe activity sensors developed for p90 ribosomal S6 kinase (RSK) and mitogen-activated protein kinases (MAPKs: ERK, p38, and JNK) whose substrate phosphorylation is known to depend on kinase-docking-groove-mediated protein-protein binding. The in vitro assays were based on fragment complementation of the NanoBit luciferase, which is facilitated upon substrate motif phosphorylation. The new phosphorylation-assisted luciferase complementation (PhALC) sensors are highly selective and the PhALC assay is a useful tool for the quantitative analysis of kinase activity or kinase docking, and even for high-throughput screening of academic compound collections." @default.
- W4387302408 created "2023-10-04" @default.
- W4387302408 creator A5008286260 @default.
- W4387302408 creator A5025253938 @default.
- W4387302408 creator A5035402577 @default.
- W4387302408 creator A5049864337 @default.
- W4387302408 creator A5051735885 @default.
- W4387302408 creator A5067383234 @default.
- W4387302408 creator A5068951002 @default.
- W4387302408 date "2023-10-03" @default.
- W4387302408 modified "2023-10-17" @default.
- W4387302408 title "Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein–Protein Binding" @default.
- W4387302408 cites W1489171502 @default.
- W4387302408 cites W1550113325 @default.
- W4387302408 cites W1625702540 @default.
- W4387302408 cites W1993315330 @default.
- W4387302408 cites W2000295215 @default.
- W4387302408 cites W2003516462 @default.
- W4387302408 cites W2007832743 @default.
- W4387302408 cites W2020513008 @default.
- W4387302408 cites W2022095303 @default.
- W4387302408 cites W2033005482 @default.
- W4387302408 cites W2033036465 @default.
- W4387302408 cites W2039201738 @default.
- W4387302408 cites W2050779639 @default.
- W4387302408 cites W2052750950 @default.
- W4387302408 cites W2057358596 @default.
- W4387302408 cites W2061699263 @default.
- W4387302408 cites W2068652881 @default.
- W4387302408 cites W2073113196 @default.
- W4387302408 cites W2076629995 @default.
- W4387302408 cites W2083117403 @default.
- W4387302408 cites W2096931514 @default.
- W4387302408 cites W2103831357 @default.
- W4387302408 cites W2116386978 @default.
- W4387302408 cites W2121438864 @default.
- W4387302408 cites W2134824357 @default.
- W4387302408 cites W2151697120 @default.
- W4387302408 cites W2159056768 @default.
- W4387302408 cites W2162474938 @default.
- W4387302408 cites W2162997245 @default.
- W4387302408 cites W2170444099 @default.
- W4387302408 cites W2226712470 @default.
- W4387302408 cites W2316178659 @default.
- W4387302408 cites W2323034105 @default.
- W4387302408 cites W2430244088 @default.
- W4387302408 cites W2497333816 @default.
- W4387302408 cites W2700931938 @default.
- W4387302408 cites W2791221265 @default.
- W4387302408 cites W2908089242 @default.
- W4387302408 cites W2943923895 @default.
- W4387302408 cites W2951976042 @default.
- W4387302408 cites W2969266131 @default.
- W4387302408 cites W2989650880 @default.
- W4387302408 cites W2990996987 @default.
- W4387302408 cites W2993892456 @default.
- W4387302408 cites W3036759197 @default.
- W4387302408 cites W3056663096 @default.
- W4387302408 cites W3087769169 @default.
- W4387302408 cites W3096333766 @default.
- W4387302408 cites W3103753279 @default.
- W4387302408 cites W3122470240 @default.
- W4387302408 cites W3161074857 @default.
- W4387302408 cites W4210479541 @default.
- W4387302408 cites W4247904703 @default.
- W4387302408 cites W4282571817 @default.
- W4387302408 cites W4284992638 @default.
- W4387302408 cites W4309184823 @default.
- W4387302408 cites W4309352938 @default.
- W4387302408 cites W4315642941 @default.
- W4387302408 cites W4380995316 @default.
- W4387302408 doi "https://doi.org/10.3390/ijms241914854" @default.
- W4387302408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37834301" @default.
- W4387302408 hasPublicationYear "2023" @default.
- W4387302408 type Work @default.
- W4387302408 citedByCount "0" @default.
- W4387302408 crossrefType "journal-article" @default.
- W4387302408 hasAuthorship W4387302408A5008286260 @default.
- W4387302408 hasAuthorship W4387302408A5025253938 @default.
- W4387302408 hasAuthorship W4387302408A5035402577 @default.
- W4387302408 hasAuthorship W4387302408A5049864337 @default.
- W4387302408 hasAuthorship W4387302408A5051735885 @default.
- W4387302408 hasAuthorship W4387302408A5067383234 @default.
- W4387302408 hasAuthorship W4387302408A5068951002 @default.
- W4387302408 hasBestOaLocation W43873024081 @default.
- W4387302408 hasConcept C108636557 @default.
- W4387302408 hasConcept C11960822 @default.
- W4387302408 hasConcept C124160383 @default.
- W4387302408 hasConcept C137361374 @default.
- W4387302408 hasConcept C159110408 @default.
- W4387302408 hasConcept C159479382 @default.
- W4387302408 hasConcept C163035849 @default.
- W4387302408 hasConcept C184235292 @default.
- W4387302408 hasConcept C185592680 @default.
- W4387302408 hasConcept C196347352 @default.
- W4387302408 hasConcept C41685203 @default.
- W4387302408 hasConcept C55493867 @default.
- W4387302408 hasConcept C59143045 @default.