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- W4387302569 endingPage "14865" @default.
- W4387302569 startingPage "14865" @default.
- W4387302569 abstract "CRISPR/Cas9-mediated cleavage of DNA, which depends on the endonuclease activity of Cas9, has been widely used for gene editing due to its excellent programmability and specificity. However, the changes to the DNA sequence that are mediated by CRISPR/Cas9 affect the structures and stability of the genome, which may affect the accuracy of results. Mutations in the RuvC and HNH regions of the Cas9 protein lead to the inactivation of Cas9 into dCas9 with no endonuclease activity. Despite the loss of endonuclease activity, dCas9 can still bind the DNA strand using guide RNA. Recently, proteins with active/inhibitory effects have been linked to the end of the dCas9 protein to form fusion proteins with transcriptional active/inhibitory effects, named CRISPRa and CRISPRi, respectively. These CRISPR tools mediate the transcription activity of protein-coding and non-coding genes by regulating the chromosomal modification states of target gene promoters, enhancers, and other functional elements. Here, we highlight the epigenetic mechanisms and applications of the common CRISPR/dCas9 tools, by which we hope to provide a reference for future related gene regulation, gene function, high-throughput target gene screening, and disease treatment." @default.
- W4387302569 created "2023-10-04" @default.
- W4387302569 creator A5021827162 @default.
- W4387302569 creator A5036622335 @default.
- W4387302569 creator A5046013419 @default.
- W4387302569 creator A5073370105 @default.
- W4387302569 creator A5076948208 @default.
- W4387302569 date "2023-10-03" @default.
- W4387302569 modified "2023-10-17" @default.
- W4387302569 title "CRISPR/dCas9 Tools: Epigenetic Mechanism and Application in Gene Transcriptional Regulation" @default.
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