Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387336301> ?p ?o ?g. }
- W4387336301 endingPage "155128" @default.
- W4387336301 startingPage "155128" @default.
- W4387336301 abstract "Pancreatic cancer is an extremely malignant digestive tumor; however, owing to its high drug resistance of pancreatic cancer, the search for more effective anti-pancreatic cancer drugs is urgently needed. Lycorine, an alkaloid of natural plant origin, exerts antitumor effects on a variety of tumors. This study aimed to investigate the therapeutic effect of lycorine on pancreatic cancer and elucidate its potential molecular mechanism. Two pancreatic cancer cell lines, PANC-1 and BxPC-3, were used to investigate the therapeutic effects of lycorine on pancreatic cancer in vitro using the CCK8 assay, colony formation assay, 5-Ethynyl-2’- deoxyuridine (EdU) incorporation assay, flow cytometry, and western blotting. Transcriptome sequencing and gene set enrichment analysis (GSEA) were used to analyze the differentially expressed genes and pathways after lycorine treatment. Molecular docking, quantitative real-time PCR (qRT-PCR), oil red O staining, small interfering RNA (siRNA) transfection, and other experiments were performed to further validate the differentially expressed genes and pathways. In vivo experiments were conducted to investigate lycorine's inhibitory effects and toxicity on pancreatic cancer using a tumor-bearing mouse model. Lycorine inhibited the proliferation of pancreatic cancer cells, caused G2/M phase cycle arrest and induced apoptosis. Transcriptome sequencing and GSEA showed that lycorine inhibition of pancreatic cancer was associated with fatty acid metabolism, and aldehyde dehydrogenase 3A1 (ALDH3A1) was a significantly enriched target in the fatty acid metabolism process. ALDH3A1 expression was significantly upregulated in pancreatic cancer and was closely associated with prognosis. Molecular docking showed that lycorine binds strongly to ALDH3A1. Further studies revealed that lycorine inhibited the fatty acid oxidation (FAO) process in pancreatic cancer cells and induced cell growth inhibition and apoptosis through ALDH3A1. Lycorine also showed significant suppressive effects in tumor-bearing mice. Importantly, it did not result in significant toxicity to liver and kidney of mice, demonstrating its therapeutic potential as a safe antitumor agent. Lycorine inhibited pancreatic cancer cell proliferation, blocked the cell cycle, and induced apoptosis by targeting ALDH3A1. FAO inhibition was identified for the first time as a possible mechanism for the anticancer effects of lycorine. These findings enrich the theory of targeted therapy for pancreatic cancer, expand our understanding of the pharmacological targets of lycorine, and provide a reference for exploring its natural components." @default.
- W4387336301 created "2023-10-05" @default.
- W4387336301 creator A5003642180 @default.
- W4387336301 creator A5006470519 @default.
- W4387336301 creator A5014079192 @default.
- W4387336301 creator A5020146416 @default.
- W4387336301 creator A5042182955 @default.
- W4387336301 creator A5047862807 @default.
- W4387336301 creator A5057349325 @default.
- W4387336301 creator A5089986454 @default.
- W4387336301 date "2024-01-01" @default.
- W4387336301 modified "2023-10-17" @default.
- W4387336301 title "Transcriptomics and Molecular Docking Reveal the Potential Mechanism of Lycorine Against Pancreatic Cancer" @default.
- W4387336301 cites W1558276741 @default.
- W4387336301 cites W1973255896 @default.
- W4387336301 cites W1975311527 @default.
- W4387336301 cites W1992799260 @default.
- W4387336301 cites W2023217479 @default.
- W4387336301 cites W2056633943 @default.
- W4387336301 cites W2065519410 @default.
- W4387336301 cites W2153478782 @default.
- W4387336301 cites W2165428601 @default.
- W4387336301 cites W2168840812 @default.
- W4387336301 cites W2254771637 @default.
- W4387336301 cites W2399064224 @default.
- W4387336301 cites W2473591635 @default.
- W4387336301 cites W2527938089 @default.
- W4387336301 cites W2885029979 @default.
- W4387336301 cites W2887543213 @default.
- W4387336301 cites W2921145883 @default.
- W4387336301 cites W2937346106 @default.
- W4387336301 cites W2962427338 @default.
- W4387336301 cites W2965716756 @default.
- W4387336301 cites W2972602909 @default.
- W4387336301 cites W2979539500 @default.
- W4387336301 cites W2984107097 @default.
- W4387336301 cites W3007291424 @default.
- W4387336301 cites W3116521081 @default.
- W4387336301 cites W3119952098 @default.
- W4387336301 cites W3120949185 @default.
- W4387336301 cites W3126393481 @default.
- W4387336301 cites W3128064198 @default.
- W4387336301 cites W3157598024 @default.
- W4387336301 cites W3173746096 @default.
- W4387336301 cites W3177452448 @default.
- W4387336301 cites W3179084948 @default.
- W4387336301 cites W3184439942 @default.
- W4387336301 cites W3184820957 @default.
- W4387336301 cites W3184894660 @default.
- W4387336301 cites W3200090337 @default.
- W4387336301 cites W4211253487 @default.
- W4387336301 cites W4220922577 @default.
- W4387336301 cites W4221075985 @default.
- W4387336301 cites W4223590989 @default.
- W4387336301 cites W4225434005 @default.
- W4387336301 cites W4229458723 @default.
- W4387336301 cites W4280527222 @default.
- W4387336301 cites W4285806028 @default.
- W4387336301 cites W4293109054 @default.
- W4387336301 cites W4295902528 @default.
- W4387336301 cites W4322626977 @default.
- W4387336301 doi "https://doi.org/10.1016/j.phymed.2023.155128" @default.
- W4387336301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37839227" @default.
- W4387336301 hasPublicationYear "2024" @default.
- W4387336301 type Work @default.
- W4387336301 citedByCount "0" @default.
- W4387336301 crossrefType "journal-article" @default.
- W4387336301 hasAuthorship W4387336301A5003642180 @default.
- W4387336301 hasAuthorship W4387336301A5006470519 @default.
- W4387336301 hasAuthorship W4387336301A5014079192 @default.
- W4387336301 hasAuthorship W4387336301A5020146416 @default.
- W4387336301 hasAuthorship W4387336301A5042182955 @default.
- W4387336301 hasAuthorship W4387336301A5047862807 @default.
- W4387336301 hasAuthorship W4387336301A5057349325 @default.
- W4387336301 hasAuthorship W4387336301A5089986454 @default.
- W4387336301 hasBestOaLocation W43873363011 @default.
- W4387336301 hasConcept C104317684 @default.
- W4387336301 hasConcept C121608353 @default.
- W4387336301 hasConcept C150194340 @default.
- W4387336301 hasConcept C153911025 @default.
- W4387336301 hasConcept C162317418 @default.
- W4387336301 hasConcept C2777667214 @default.
- W4387336301 hasConcept C2780210213 @default.
- W4387336301 hasConcept C2781465977 @default.
- W4387336301 hasConcept C502942594 @default.
- W4387336301 hasConcept C54355233 @default.
- W4387336301 hasConcept C55493867 @default.
- W4387336301 hasConcept C59822182 @default.
- W4387336301 hasConcept C86803240 @default.
- W4387336301 hasConcept C96232424 @default.
- W4387336301 hasConceptScore W4387336301C104317684 @default.
- W4387336301 hasConceptScore W4387336301C121608353 @default.
- W4387336301 hasConceptScore W4387336301C150194340 @default.
- W4387336301 hasConceptScore W4387336301C153911025 @default.
- W4387336301 hasConceptScore W4387336301C162317418 @default.
- W4387336301 hasConceptScore W4387336301C2777667214 @default.
- W4387336301 hasConceptScore W4387336301C2780210213 @default.
- W4387336301 hasConceptScore W4387336301C2781465977 @default.