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• W4387344186 abstract "The biopanning of target-specific phages is one of the most critical steps in the preparation of single-domain antibodies. In the traditional biopanning of haptens, the nonspecific binding of library phages to macromolecular proteins is one of the most challenging problems in preparing single-domain antibodies. In this research, Fe3O4@ENR-functionalized core-shell magnetic nanoparticles (FMNPs) were silylated and aminated by tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane, and target enrofloxacin was coupled onto the surface by the carbodiimide method. The magnetic nanoparticles were characterized by Fourier transform infrared spectroscopy, particle size distribution, zeta potential, transmission electron microscopy observation, and indirect enzyme-linked immunosorbent assay (ELISA). A biopanning strategy based on Fe3O4@ENR FMNPs was then established to solve the problem in the traditional solid-phase biopanning process. The results showed that a considerable number of enrofloxacin (ENR)-positive phages were screened by only one round of biopanning. Finally, two ENR-specific shark-derived single-domain genes were identified and validated by monoclonal phage ELISA, gene sequencing, and biolayer interferometry technology. Our study provides a new biopanning strategy based on Fe3O4@ENR FMNPs for efficiently providing phages specific to haptens." @default.
• W4387344186 created "2023-10-05" @default.
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• W4387344186 date "2023-10-04" @default.
• W4387344186 modified "2023-10-18" @default.
• W4387344186 title "Efficient Hapten-Specific Biopanning Strategy Based on the Fe₃O₄@ENR-Functionalized Core–Shell Magnetic Nanoparticles" @default.
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• W4387344186 doi "https://doi.org/10.1021/acs.langmuir.3c01803" @default.
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• W4387344186 hasPublicationYear "2023" @default.
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