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- W4387345449 abstract "Microinjection, which is widely employed in modern biology and medicine research, is a crucial method for transferring biomaterial in a single cell. Despite substantial research into microinjection automation technology, how to achieve high precision, which is crucial for quantitative analysis, has gotten scant attention. This paper offers a dual-loop control syringe-based picoliter-scale injection system, with the inner being a pneumatic servo loop with a baroreceptor providing pressure feedback and the outside being a position servo loop with a microscope providing visual feedback. Specifically, significant crawling phenomenon are found throughout the characteristic test trial, rendering the gas-liquid interface uncontrollable. To mathematically characterize this phenomenon, a theoretical model is developed that accounts for static friction, capillary force, capillary cone angle, and fluid viscous force. The genetic algorithm is used to identify difficult-to-measure and calibrate parameters. Based on the established model, an integral sliding-mode controller (ISMC) with saturation function and a PID controller for the inside loop control were established, both of which have a fast convergence rate and do not overshoot. Finally, experimentation is used to validate the designed cell injection system, demonstrating that the suggested system can regulate the smallest droplet in the tip of the needle to within 0.25 pL." @default.
- W4387345449 created "2023-10-05" @default.
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- W4387345449 date "2024-01-01" @default.
- W4387345449 modified "2023-10-09" @default.
- W4387345449 title "Development, modeling, and control of a syringe-based picoliter-scale microinjection system" @default.
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- W4387345449 doi "https://doi.org/10.1016/j.precisioneng.2023.09.011" @default.
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