Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387346481> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W4387346481 abstract "Recently, much work has been done on chemical probing strategies with nanopore sequencing to identify RNA modifications at the single nucleotide level. Here, we examine the use of Oxford Nanopore's Guppy basecalling to identify structural modifications using localized, non-parametric peak detection. In a novel experiment, we evaluate whether detection of structural modifications is possible using the Guppy's basecalling error and determine the accuracy of our approach for selected RNA control sequences. Next, we use statistical analysis to determine the dominant structural bindings in a set of averaged read errors. Finally, we compare our approach to average reactivity determined by orthogonal experiments from SHAPE-CE and alternative approaches. We show that localized, non-parametric peak detection demonstrates improved accuracy and coverage of structural modifications in selected control RNA and that our method is agnostic to underlying changes in the distribution. Our approach allows for a more generalizeable methodology for detecting structural modification with nanopore sequencing and the subsequent generated probabilities can be used to refine further downstream analysis." @default.
- W4387346481 created "2023-10-05" @default.
- W4387346481 creator A5000881484 @default.
- W4387346481 creator A5018570031 @default.
- W4387346481 creator A5044474048 @default.
- W4387346481 creator A5088084000 @default.
- W4387346481 date "2023-09-03" @default.
- W4387346481 modified "2023-10-06" @default.
- W4387346481 title "Localized, Non-parametric Detection of RNA Structural Modification using Nanopore Basecalling." @default.
- W4387346481 cites W1972533328 @default.
- W4387346481 cites W1972978214 @default.
- W4387346481 cites W1993403357 @default.
- W4387346481 cites W2066735930 @default.
- W4387346481 cites W2147362276 @default.
- W4387346481 cites W2762250455 @default.
- W4387346481 cites W2789843538 @default.
- W4387346481 cites W2884297880 @default.
- W4387346481 cites W2950993016 @default.
- W4387346481 cites W2971454256 @default.
- W4387346481 cites W2999689892 @default.
- W4387346481 cites W3043981686 @default.
- W4387346481 cites W3094287895 @default.
- W4387346481 cites W3131055609 @default.
- W4387346481 cites W3164139532 @default.
- W4387346481 cites W3164201909 @default.
- W4387346481 cites W3175727154 @default.
- W4387346481 cites W4221122360 @default.
- W4387346481 cites W4223962268 @default.
- W4387346481 cites W4224227778 @default.
- W4387346481 cites W4255375128 @default.
- W4387346481 cites W4297230967 @default.
- W4387346481 cites W4306970980 @default.
- W4387346481 doi "https://doi.org/10.1145/3584371.3612989" @default.
- W4387346481 hasPublicationYear "2023" @default.
- W4387346481 type Work @default.
- W4387346481 citedByCount "0" @default.
- W4387346481 crossrefType "proceedings-article" @default.
- W4387346481 hasAuthorship W4387346481A5000881484 @default.
- W4387346481 hasAuthorship W4387346481A5018570031 @default.
- W4387346481 hasAuthorship W4387346481A5044474048 @default.
- W4387346481 hasAuthorship W4387346481A5088084000 @default.
- W4387346481 hasBestOaLocation W43873464811 @default.
- W4387346481 hasConcept C104317684 @default.
- W4387346481 hasConcept C105795698 @default.
- W4387346481 hasConcept C11413529 @default.
- W4387346481 hasConcept C117251300 @default.
- W4387346481 hasConcept C126513998 @default.
- W4387346481 hasConcept C141795571 @default.
- W4387346481 hasConcept C171250308 @default.
- W4387346481 hasConcept C186060115 @default.
- W4387346481 hasConcept C192562407 @default.
- W4387346481 hasConcept C33923547 @default.
- W4387346481 hasConcept C41008148 @default.
- W4387346481 hasConcept C51679486 @default.
- W4387346481 hasConcept C54355233 @default.
- W4387346481 hasConcept C67705224 @default.
- W4387346481 hasConcept C70721500 @default.
- W4387346481 hasConcept C86803240 @default.
- W4387346481 hasConceptScore W4387346481C104317684 @default.
- W4387346481 hasConceptScore W4387346481C105795698 @default.
- W4387346481 hasConceptScore W4387346481C11413529 @default.
- W4387346481 hasConceptScore W4387346481C117251300 @default.
- W4387346481 hasConceptScore W4387346481C126513998 @default.
- W4387346481 hasConceptScore W4387346481C141795571 @default.
- W4387346481 hasConceptScore W4387346481C171250308 @default.
- W4387346481 hasConceptScore W4387346481C186060115 @default.
- W4387346481 hasConceptScore W4387346481C192562407 @default.
- W4387346481 hasConceptScore W4387346481C33923547 @default.
- W4387346481 hasConceptScore W4387346481C41008148 @default.
- W4387346481 hasConceptScore W4387346481C51679486 @default.
- W4387346481 hasConceptScore W4387346481C54355233 @default.
- W4387346481 hasConceptScore W4387346481C67705224 @default.
- W4387346481 hasConceptScore W4387346481C70721500 @default.
- W4387346481 hasConceptScore W4387346481C86803240 @default.
- W4387346481 hasLocation W43873464811 @default.
- W4387346481 hasOpenAccess W4387346481 @default.
- W4387346481 hasPrimaryLocation W43873464811 @default.
- W4387346481 hasRelatedWork W2509093936 @default.
- W4387346481 hasRelatedWork W2950636974 @default.
- W4387346481 hasRelatedWork W3109164186 @default.
- W4387346481 hasRelatedWork W3146423745 @default.
- W4387346481 hasRelatedWork W4210751374 @default.
- W4387346481 hasRelatedWork W4293199644 @default.
- W4387346481 hasRelatedWork W4296381520 @default.
- W4387346481 hasRelatedWork W4302362471 @default.
- W4387346481 hasRelatedWork W4321996631 @default.
- W4387346481 hasRelatedWork W4362698352 @default.
- W4387346481 isParatext "false" @default.
- W4387346481 isRetracted "false" @default.
- W4387346481 workType "article" @default.