FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387361182> ?p ?o ?g. }

• W4387361182 abstract "Abstract Disclosure: S. Rafiqi: None. A. Aldasouqi: None. S. faisal: None. S. Qureshi: None. A. Mahmood: None. J.C. Jaume: None. S. Imam: None. We explored immune resetting and plasticity of T effector cells towards T regulatory cells (CD4+CD25+FOXP3+) by elF5A and Notch inhibition. We validated our approach for plasticizing human T effector cells into Treg cells conducted on recent onset latent autoimmune diabetes of adult (LADA) patients (n=3), and nondiabetic patients (n=4) with simultaneous use of GC7 (100 μM) and anti-DLL4 (10 μg/ml). We also investigated the functional stability of plasticized Tregs and compared it with freshly isolated naïve Tregs from the same patient. Tregs were co-cultured with T effector cells and suppression/proliferation was accessed after 5 days. To create an ex-vivo Treg deficient environment, we sorted out CD4+ cells from the PBMCs. CD4+ deficient PBMCs and sorted single positive CD4+CD25- cells were treated with anti-DLL4+GC7+rhGAD65 with and without IL-2 supplementation and were compared with conventional anti-(CD3+CD28) stimulation and absolute control (20%FCS+RPMI). The presence of Treg cells was confirmed by flow cytometry. 30.5±5.1% of CD4 cells were plasticized into Tregs in the treatment group supplemented with IL-2 while only 18.8±4.6 % were plasticized in treatment group without IL-2 supplementation. Also, 11.2±3.1% plasticized to Tregs in treatment group in presence of CD3/CD28 microbeads compared to 6.19±2.4% in absolute control group. Similarly, 13±4.3% of CD8 cells had plasticized into Tregs in treatment group supplemented with IL-2 while only 11.5±6.7% plasticized in treatment group without IL-2 supplementation. 14.3±5.8% plasticized to Tregs in treatment group in presence of CD3/CD28 microbeads compared to 12.4±6.3% in absolute control group. Interestingly, the plasticized Tregs from LADA patients were more robust than those of non-diabetic patients at a 1:2 ratio (Treg:Tresp). Furthermore, the plasticized Tregs had comparable suppressive capacity over Tresp (CD4+CD25-) cells as that of freshly isolated naïve human Tregs. To investigate any off-target effects of (GC7 and anti-DLL4), live, dead, and apoptotic cell count at 24, 48, 96 hours, and 7 days post-treatment using dead cell apoptosis kit (Invitrogen). In our study, there was no significant difference between treatment and control groups in terms of live, dead, and apoptotic cells at different time intervals. On the 7th day of culture 64.06±8.66% and 68.88±6.15% of cells were live in treatment and absolute control groups respectively while 7.24±1.59% and 10.0±2.02% cells were found apoptotic respectively. Therefore, we define the reprogramming of T effector cells towards Treg cells with functional suppressive capacity using GC7 and anti-DLL4 without any off-target effect (toxicity). The present study expands the concept of T cell plasticity and establishes the functional stability of plasticized T cells that can accelerate pre-clinical trials for therapeutic use in T1D and as an adjunct to immunotherapies. Presentation: Friday, June 16, 2023" @default.
• W4387361182 created "2023-10-06" @default.
• W4387361182 creator A5007297883 @default.
• W4387361182 creator A5009973048 @default.
• W4387361182 creator A5026935900 @default.
• W4387361182 creator A5055269698 @default.
• W4387361182 creator A5072879857 @default.
• W4387361182 creator A5084978172 @default.
• W4387361182 creator A5093007138 @default.
• W4387361182 date "2023-10-01" @default.
• W4387361182 modified "2023-10-06" @default.
• W4387361182 title "FRI616 Combined Elf5a And Notch Inhibition Reprograms The Effector T Cell Fate Decisions Towards Treg Phenotype Without Any Off Target Effect" @default.
• W4387361182 doi "https://doi.org/10.1210/jendso/bvad114.838" @default.
• W4387361182 hasPublicationYear "2023" @default.
• W4387361182 type Work @default.
• W4387361182 citedByCount "0" @default.
• W4387361182 crossrefType "journal-article" @default.
• W4387361182 hasAuthorship W4387361182A5007297883 @default.
• W4387361182 hasAuthorship W4387361182A5009973048 @default.
• W4387361182 hasAuthorship W4387361182A5026935900 @default.
• W4387361182 hasAuthorship W4387361182A5055269698 @default.
• W4387361182 hasAuthorship W4387361182A5072879857 @default.
• W4387361182 hasAuthorship W4387361182A5084978172 @default.
• W4387361182 hasAuthorship W4387361182A5093007138 @default.
• W4387361182 hasBestOaLocation W43873611821 @default.
• W4387361182 hasConcept C114684123 @default.
• W4387361182 hasConcept C137061746 @default.
• W4387361182 hasConcept C148125776 @default.
• W4387361182 hasConcept C167672396 @default.
• W4387361182 hasConcept C185592680 @default.
• W4387361182 hasConcept C202751555 @default.
• W4387361182 hasConcept C203014093 @default.
• W4387361182 hasConcept C2776090121 @default.
• W4387361182 hasConcept C2779727006 @default.
• W4387361182 hasConcept C502942594 @default.
• W4387361182 hasConcept C51785407 @default.
• W4387361182 hasConcept C553184892 @default.
• W4387361182 hasConcept C55493867 @default.
• W4387361182 hasConcept C71924100 @default.
• W4387361182 hasConcept C79484868 @default.
• W4387361182 hasConcept C86803240 @default.
• W4387361182 hasConcept C8891405 @default.
• W4387361182 hasConcept C95444343 @default.
• W4387361182 hasConceptScore W4387361182C114684123 @default.
• W4387361182 hasConceptScore W4387361182C137061746 @default.
• W4387361182 hasConceptScore W4387361182C148125776 @default.
• W4387361182 hasConceptScore W4387361182C167672396 @default.
• W4387361182 hasConceptScore W4387361182C185592680 @default.
• W4387361182 hasConceptScore W4387361182C202751555 @default.
• W4387361182 hasConceptScore W4387361182C203014093 @default.
• W4387361182 hasConceptScore W4387361182C2776090121 @default.
• W4387361182 hasConceptScore W4387361182C2779727006 @default.
• W4387361182 hasConceptScore W4387361182C502942594 @default.
• W4387361182 hasConceptScore W4387361182C51785407 @default.
• W4387361182 hasConceptScore W4387361182C553184892 @default.
• W4387361182 hasConceptScore W4387361182C55493867 @default.
• W4387361182 hasConceptScore W4387361182C71924100 @default.
• W4387361182 hasConceptScore W4387361182C79484868 @default.
• W4387361182 hasConceptScore W4387361182C86803240 @default.
• W4387361182 hasConceptScore W4387361182C8891405 @default.
• W4387361182 hasConceptScore W4387361182C95444343 @default.
• W4387361182 hasIssue "Supplement_1" @default.
• W4387361182 hasLocation W43873611821 @default.
• W4387361182 hasOpenAccess W4387361182 @default.
• W4387361182 hasPrimaryLocation W43873611821 @default.
• W4387361182 hasRelatedWork W1557245755 @default.
• W4387361182 hasRelatedWork W1585751203 @default.
• W4387361182 hasRelatedWork W1990002777 @default.
• W4387361182 hasRelatedWork W1997185845 @default.
• W4387361182 hasRelatedWork W2354113403 @default.
• W4387361182 hasRelatedWork W2389761496 @default.
• W4387361182 hasRelatedWork W2474797438 @default.
• W4387361182 hasRelatedWork W2560768058 @default.
• W4387361182 hasRelatedWork W4200177251 @default.
• W4387361182 hasRelatedWork W4295086901 @default.
• W4387361182 hasVolume "7" @default.
• W4387361182 isParatext "false" @default.
• W4387361182 isRetracted "false" @default.
• W4387361182 workType "article" @default.