Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387367181> ?p ?o ?g. }
- W4387367181 endingPage "116689" @default.
- W4387367181 startingPage "116689" @default.
- W4387367181 abstract "Pogostemon cablin (patchouli) is one of the most prized medicinal herbs cultivated worldwide. However, with changes in the bioclimatic patterns and nematode infections in its root, the availability of quality planting material for essential oil extraction has become a challenging task in recent times. In the present research, a synergistic effort based on the identification of the optimal gene expression level of the patchoulol synthase (PS) along with fresh weight (FW), an effective strategy was formulated for the bioprospection of specialized metabolites of patchouli via plant tissue culture based in vitro approaches. The comparative expression levels of the PS gene in various tissues grown in semisolid as well as suspension cultures provided vital insights into its role in the biosynthesis of patchoulol and its associated compounds. Comparatively, cell suspension derived biomass exhibited better efficacy in terms of antioxidant activity (IC50 = 27.88 μg/ml), sun protection factor (SPF = 4.49–20.20), accumulation of both volatile and non-volatile metabolites profiled through ultra performance liquid chromatography and gas chromatography-mass spectrometry (GC-MS) along with up regulated PS gene activity (5.01 FC). Interestingly, semisolid culture media derived callus extract also exhibited potent activities establishing the novelty of the approach. The findings illustrate the utilization of aromatic cytokinin – meta-Topolin as a potent elicitor in synergy with conventional elicitors like methyl jasmonate and synthetic growth regulators such as thidiazuron. The current approach also provides vital, reproducible, sustainable and cost-effective method for the bioprospection of prized metabolites in the callus and cell suspension cultures with the goal of up-scaling to pilot scale via coordinated use of next generation bioreactor approaches." @default.
- W4387367181 created "2023-10-06" @default.
- W4387367181 creator A5000286105 @default.
- W4387367181 creator A5010643028 @default.
- W4387367181 creator A5011576036 @default.
- W4387367181 creator A5036256284 @default.
- W4387367181 creator A5071643601 @default.
- W4387367181 creator A5083149918 @default.
- W4387367181 date "2023-12-01" @default.
- W4387367181 modified "2023-10-15" @default.
- W4387367181 title "Metabolite bioprospection and expression analysis of patchoulol synthase gene in different in vitro systems of Pogostemon cablin: An important medicinal aromatic plant" @default.
- W4387367181 cites W1189460543 @default.
- W4387367181 cites W1540044642 @default.
- W4387367181 cites W1549839546 @default.
- W4387367181 cites W1780638118 @default.
- W4387367181 cites W1784815923 @default.
- W4387367181 cites W1928707112 @default.
- W4387367181 cites W1968954571 @default.
- W4387367181 cites W1972004106 @default.
- W4387367181 cites W1974446623 @default.
- W4387367181 cites W1983159130 @default.
- W4387367181 cites W1985423404 @default.
- W4387367181 cites W1985431229 @default.
- W4387367181 cites W1991679971 @default.
- W4387367181 cites W1993797925 @default.
- W4387367181 cites W1996147024 @default.
- W4387367181 cites W1996248058 @default.
- W4387367181 cites W1996676025 @default.
- W4387367181 cites W2001664558 @default.
- W4387367181 cites W2006670683 @default.
- W4387367181 cites W2008777824 @default.
- W4387367181 cites W2015531760 @default.
- W4387367181 cites W2015751517 @default.
- W4387367181 cites W2018495988 @default.
- W4387367181 cites W2021360131 @default.
- W4387367181 cites W2023505657 @default.
- W4387367181 cites W2024556021 @default.
- W4387367181 cites W2033208203 @default.
- W4387367181 cites W2039250537 @default.
- W4387367181 cites W2045082656 @default.
- W4387367181 cites W2045805733 @default.
- W4387367181 cites W2056213698 @default.
- W4387367181 cites W2060041263 @default.
- W4387367181 cites W2067931902 @default.
- W4387367181 cites W2069474296 @default.
- W4387367181 cites W2074066688 @default.
- W4387367181 cites W2083172349 @default.
- W4387367181 cites W2084046958 @default.
- W4387367181 cites W2084244231 @default.
- W4387367181 cites W2086082921 @default.
- W4387367181 cites W2088581304 @default.
- W4387367181 cites W2092003667 @default.
- W4387367181 cites W2092713296 @default.
- W4387367181 cites W2094778259 @default.
- W4387367181 cites W2099667688 @default.
- W4387367181 cites W2104251467 @default.
- W4387367181 cites W2109819239 @default.
- W4387367181 cites W2114570899 @default.
- W4387367181 cites W2116114859 @default.
- W4387367181 cites W2118477144 @default.
- W4387367181 cites W2123653483 @default.
- W4387367181 cites W2132123562 @default.
- W4387367181 cites W2134340518 @default.
- W4387367181 cites W2143195277 @default.
- W4387367181 cites W2145937448 @default.
- W4387367181 cites W2151628777 @default.
- W4387367181 cites W2166690382 @default.
- W4387367181 cites W2172102325 @default.
- W4387367181 cites W2280621788 @default.
- W4387367181 cites W2337083032 @default.
- W4387367181 cites W2390012465 @default.
- W4387367181 cites W2468334735 @default.
- W4387367181 cites W2468835921 @default.
- W4387367181 cites W2525238508 @default.
- W4387367181 cites W2529407431 @default.
- W4387367181 cites W2549719401 @default.
- W4387367181 cites W2610846459 @default.
- W4387367181 cites W2623740430 @default.
- W4387367181 cites W2734344812 @default.
- W4387367181 cites W2784133181 @default.
- W4387367181 cites W2790254882 @default.
- W4387367181 cites W2790553502 @default.
- W4387367181 cites W2886042057 @default.
- W4387367181 cites W2901159587 @default.
- W4387367181 cites W2901694035 @default.
- W4387367181 cites W2903210939 @default.
- W4387367181 cites W2913484606 @default.
- W4387367181 cites W2994754326 @default.
- W4387367181 cites W2995585247 @default.
- W4387367181 cites W3012702001 @default.
- W4387367181 cites W3038227151 @default.
- W4387367181 cites W3044008467 @default.
- W4387367181 cites W3085249443 @default.
- W4387367181 cites W3092096422 @default.
- W4387367181 cites W3136624969 @default.
- W4387367181 cites W3165672187 @default.
- W4387367181 cites W3191326478 @default.
- W4387367181 cites W3198544462 @default.