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- W4387372488 abstract "As a holoparasitic weed, broomrape has seriously threatened the production of economically important crops, such as melon, watermelon, processed tomato, and sunflower, in the Xinjiang Province in recent years. However, the distribution and genetic diversity of broomrape populations in Xinjiang are not clear at present, which hinders their prevention and control process. In the present study, 93 samples from different geographic regions of Xinjiang were collected to identify the species based on ITS and plastid rps2 regions, and the samples were used to analyze the genetic diversity based on ISSR markers. The results showed that broomrape is not monophyletic in Xinjiang and consists of two major clades (Orobanche cf. aegyptiaca and O. cernua) and three subclades (O. cf. aegyptiaca var. tch, O. cf. aegyptiaca var. klz, O. cernua.var. alt) by phylogenetic analysis based on ITS and rps2. Furthermore, the results of the genetic diversity analysis indicated that the 11 selected primers produced 154 repeatable polymorphic bands, of which 150 were polymorphic. The genetic diversity of the samples was 37.19% within populations and 62.81% among the populations, indicating that the main genetic differentiation occurred among the populations. There was less gene exchange between populations, with a gene flow index (Nm) of 0.2961 (<1). The UPGMA dendrogram indicated that most populations with similar geographical conditions and hosts were clustered first, and then all samples were separated into two major groups and seven subclusters. This is the first study to clarify genetic diversity and population distribution of broomrape in Xinjiang. Our results provide a theoretical basis for the prevention, control, and breeding of broomrape-resistant varieties." @default.
- W4387372488 created "2023-10-06" @default.
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- W4387372488 date "2023-10-05" @default.
- W4387372488 modified "2023-10-16" @default.
- W4387372488 title "Identification and genetic diversity analysis of broomrape in Xinjiang province, China" @default.
- W4387372488 doi "https://doi.org/10.22541/au.169648575.57105081/v1" @default.
- W4387372488 hasPublicationYear "2023" @default.
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