Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387376479> ?p ?o ?g. }
- W4387376479 endingPage "6937" @default.
- W4387376479 startingPage "6937" @default.
- W4387376479 abstract "Glutarimide-containing polyketides exhibiting potent antitumor and antimicrobial activities were encoded via conserved module blocks in various strains that favor the genomic mining of these family compounds. The bioinformatic analysis of the genome of Burkholderia gladioli ATCC 10248 showed a silent trans-AT PKS biosynthetic gene cluster (BGC) on chromosome 2 (Chr2C8), which was predicted to produce new glutarimide-containing derivatives. Then, the silent polyketide synthase gene cluster was successfully activated via in situ promoter insertion and heterologous expression. As a result, seven glutarimide-containing analogs, including five new ones, gladiofungins D-H (3–7), and two known gladiofungin A/gladiostatin (1) and 2 (named gladiofungin C), were isolated from the fermentation of the activated mutant. Their structures were elucidated through the analysis of HR-ESI-MS and NMR spectroscopy. The structural diversities of gladiofungins may be due to the degradation of the butenolide group in gladiofungin A (1) during the fermentation and extraction process. Bioactivity screening showed that 2 and 4 had moderate anti-inflammatory activities. Thus, genome mining combined with promoter engineering and heterologous expression were proved to be effective strategies for the pathway-specific activation of the silent BGCs for the directional discovery of new natural products." @default.
- W4387376479 created "2023-10-06" @default.
- W4387376479 creator A5006923891 @default.
- W4387376479 creator A5020768419 @default.
- W4387376479 creator A5026735983 @default.
- W4387376479 creator A5036716997 @default.
- W4387376479 creator A5041432334 @default.
- W4387376479 creator A5064203235 @default.
- W4387376479 creator A5080272284 @default.
- W4387376479 date "2023-10-05" @default.
- W4387376479 modified "2023-10-18" @default.
- W4387376479 title "The Genomic-Driven Discovery of Glutarimide-Containing Derivatives from Burkholderia gladioli" @default.
- W4387376479 cites W1606461650 @default.
- W4387376479 cites W1948803169 @default.
- W4387376479 cites W1967756460 @default.
- W4387376479 cites W1968146772 @default.
- W4387376479 cites W1970270191 @default.
- W4387376479 cites W1973375098 @default.
- W4387376479 cites W1977421075 @default.
- W4387376479 cites W1977683305 @default.
- W4387376479 cites W1996752455 @default.
- W4387376479 cites W1997453147 @default.
- W4387376479 cites W2000469215 @default.
- W4387376479 cites W2000832822 @default.
- W4387376479 cites W2014874550 @default.
- W4387376479 cites W2016233397 @default.
- W4387376479 cites W2028903560 @default.
- W4387376479 cites W2045021635 @default.
- W4387376479 cites W2071982598 @default.
- W4387376479 cites W2113949943 @default.
- W4387376479 cites W2115552487 @default.
- W4387376479 cites W2139434153 @default.
- W4387376479 cites W2325918749 @default.
- W4387376479 cites W2327944389 @default.
- W4387376479 cites W2334777282 @default.
- W4387376479 cites W2346792764 @default.
- W4387376479 cites W2417585904 @default.
- W4387376479 cites W2526962005 @default.
- W4387376479 cites W2603188767 @default.
- W4387376479 cites W2771508325 @default.
- W4387376479 cites W2799615008 @default.
- W4387376479 cites W2895366230 @default.
- W4387376479 cites W2909909436 @default.
- W4387376479 cites W2913875306 @default.
- W4387376479 cites W2917010368 @default.
- W4387376479 cites W2949169092 @default.
- W4387376479 cites W2978629681 @default.
- W4387376479 cites W2995613696 @default.
- W4387376479 cites W3037382645 @default.
- W4387376479 cites W3084925804 @default.
- W4387376479 cites W3095583226 @default.
- W4387376479 cites W3123411726 @default.
- W4387376479 cites W3133838327 @default.
- W4387376479 cites W3137969676 @default.
- W4387376479 cites W3156330121 @default.
- W4387376479 cites W3162547463 @default.
- W4387376479 cites W3173984248 @default.
- W4387376479 cites W3175669157 @default.
- W4387376479 cites W3186852362 @default.
- W4387376479 cites W3205130467 @default.
- W4387376479 cites W4240919178 @default.
- W4387376479 cites W4309576546 @default.
- W4387376479 cites W4309604547 @default.
- W4387376479 cites W4368358469 @default.
- W4387376479 cites W4385443005 @default.
- W4387376479 cites W4385493961 @default.
- W4387376479 cites W81291701 @default.
- W4387376479 doi "https://doi.org/10.3390/molecules28196937" @default.
- W4387376479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37836780" @default.
- W4387376479 hasPublicationYear "2023" @default.
- W4387376479 type Work @default.
- W4387376479 citedByCount "0" @default.
- W4387376479 crossrefType "journal-article" @default.
- W4387376479 hasAuthorship W4387376479A5006923891 @default.
- W4387376479 hasAuthorship W4387376479A5020768419 @default.
- W4387376479 hasAuthorship W4387376479A5026735983 @default.
- W4387376479 hasAuthorship W4387376479A5036716997 @default.
- W4387376479 hasAuthorship W4387376479A5041432334 @default.
- W4387376479 hasAuthorship W4387376479A5064203235 @default.
- W4387376479 hasAuthorship W4387376479A5080272284 @default.
- W4387376479 hasBestOaLocation W43873764791 @default.
- W4387376479 hasConcept C104317684 @default.
- W4387376479 hasConcept C108305142 @default.
- W4387376479 hasConcept C141231307 @default.
- W4387376479 hasConcept C143065580 @default.
- W4387376479 hasConcept C185592680 @default.
- W4387376479 hasConcept C2778635478 @default.
- W4387376479 hasConcept C2780115692 @default.
- W4387376479 hasConcept C2780855054 @default.
- W4387376479 hasConcept C36857842 @default.
- W4387376479 hasConcept C40767141 @default.
- W4387376479 hasConcept C523546767 @default.
- W4387376479 hasConcept C54355233 @default.
- W4387376479 hasConcept C553450214 @default.
- W4387376479 hasConcept C55493867 @default.
- W4387376479 hasConcept C86803240 @default.
- W4387376479 hasConceptScore W4387376479C104317684 @default.
- W4387376479 hasConceptScore W4387376479C108305142 @default.