FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4387391893> ?p ?o ?g. }

• W4387391893 abstract "Abstract Background The unique epigenetic architecture that sperm cells acquire during spermiogenesis by retaining <15% of either canonical or variant histone proteins in their genome is essential for normal embryogenesis. Whilst heterogeneous levels of retained histones are found in morphologically normal spermatozoa, their effect on reproductive outcomes is not fully understood. Methods Processed spermatozoa ( n = 62) were tested for DNA integrity by sperm chromatin dispersion assay, and retained histones were extracted and subjected to dot‐blot analysis. The impact of retained histone modifications in normozoospermic patients on sperm functional characteristics, embryo quality, metabolic signature in embryo spent culture medium and pregnancy outcome was studied. Results Dot‐blot analysis showed heterogeneous levels of retained histones in the genome of normozoospermic ejaculates. Post‐wash sperm yield was affected by an increase in H3K27Me3 and H4K20Me3 levels in the sperm chromatin ( p < 0.05). Also, spermatozoa with higher histone H3 retention had increased DNA damage ( p < 0.05). Spermatozoa from these cohorts, when injected into donor oocytes, correlated to a significant decrease in the fertilisation rate with an increase in sperm histone H3 ( p < 0.05) and H3K27Me3 ( p < 0.01). An increase in histone H3 negatively affected embryo quality ( p < 0.01) and clinical pregnancy outcome post‐embryo transfer ( p < 0.05). On the other hand, spent culture medium metabolites assessed by high‐resolution (800 MHz) nuclear magnetic resonance showed an increased intensity of the amino acid methionine in the non‐pregnant group than in the pregnant group ( p < 0.05) and a negative correlation with sperm histone H3 in the pregnant group ( p < 0.05). Discussion and conclusion Histone retention in spermatozoa can be one of the factors behind the development of idiopathic male infertility. Such spermatozoa may influence embryonic behaviour and thereby affect the success rate of assisted reproductive technology procedures. These results, although descriptive in nature, warrant further research to address the underlying mechanisms behind these clinically important observations." @default.
• W4387391893 created "2023-10-07" @default.
• W4387391893 creator A5005900554 @default.
• W4387391893 creator A5006101864 @default.
• W4387391893 creator A5013658827 @default.
• W4387391893 creator A5016712439 @default.
• W4387391893 creator A5019657327 @default.
• W4387391893 creator A5021040279 @default.
• W4387391893 creator A5052294515 @default.
• W4387391893 creator A5053189716 @default.
• W4387391893 creator A5077964485 @default.
• W4387391893 creator A5088634703 @default.
• W4387391893 date "2023-10-06" @default.
• W4387391893 modified "2023-10-18" @default.
• W4387391893 title "Differential sperm histone retention in normozoospermic ejaculates of infertile men negatively affects sperm functional competence and embryo quality" @default.
• W4387391893 cites W1484972278 @default.
• W4387391893 cites W1910049290 @default.
• W4387391893 cites W1941377923 @default.
• W4387391893 cites W1966429292 @default.
• W4387391893 cites W1970093123 @default.
• W4387391893 cites W1972934701 @default.
• W4387391893 cites W1987272410 @default.
• W4387391893 cites W1993419284 @default.
• W4387391893 cites W1997672006 @default.
• W4387391893 cites W1999696239 @default.
• W4387391893 cites W2006866503 @default.
• W4387391893 cites W2010999794 @default.
• W4387391893 cites W2021831690 @default.
• W4387391893 cites W2033610051 @default.
• W4387391893 cites W2037818631 @default.
• W4387391893 cites W2051008134 @default.
• W4387391893 cites W2060863780 @default.
• W4387391893 cites W2066408612 @default.
• W4387391893 cites W2067842438 @default.
• W4387391893 cites W2068991517 @default.
• W4387391893 cites W2082444663 @default.
• W4387391893 cites W2083402858 @default.
• W4387391893 cites W2088306032 @default.
• W4387391893 cites W2088341189 @default.
• W4387391893 cites W2094119919 @default.
• W4387391893 cites W2097677113 @default.
• W4387391893 cites W2097939257 @default.
• W4387391893 cites W2099721980 @default.
• W4387391893 cites W2103410123 @default.
• W4387391893 cites W2107503821 @default.
• W4387391893 cites W2108037980 @default.
• W4387391893 cites W2108531322 @default.
• W4387391893 cites W2111075937 @default.
• W4387391893 cites W2115199535 @default.
• W4387391893 cites W2116046187 @default.
• W4387391893 cites W2124656409 @default.
• W4387391893 cites W2129160382 @default.
• W4387391893 cites W2130597092 @default.
• W4387391893 cites W2130732086 @default.
• W4387391893 cites W2141081944 @default.
• W4387391893 cites W2145291407 @default.
• W4387391893 cites W2152425292 @default.
• W4387391893 cites W2153500400 @default.
• W4387391893 cites W2155678594 @default.
• W4387391893 cites W2165854472 @default.
• W4387391893 cites W2166236051 @default.
• W4387391893 cites W2168831494 @default.
• W4387391893 cites W2195999432 @default.
• W4387391893 cites W2206043536 @default.
• W4387391893 cites W2210080033 @default.
• W4387391893 cites W2228244355 @default.
• W4387391893 cites W2271002166 @default.
• W4387391893 cites W2470358492 @default.
• W4387391893 cites W2474403743 @default.
• W4387391893 cites W2477954974 @default.
• W4387391893 cites W2482098192 @default.
• W4387391893 cites W2517448728 @default.
• W4387391893 cites W2519280707 @default.
• W4387391893 cites W2520701010 @default.
• W4387391893 cites W2765736513 @default.
• W4387391893 cites W2792412107 @default.
• W4387391893 cites W2799444814 @default.
• W4387391893 cites W2800816467 @default.
• W4387391893 cites W2802090556 @default.
• W4387391893 cites W2810157783 @default.
• W4387391893 cites W2900091173 @default.
• W4387391893 cites W2900541546 @default.
• W4387391893 cites W2921731528 @default.
• W4387391893 cites W2965103741 @default.
• W4387391893 cites W2979057541 @default.
• W4387391893 cites W2989643914 @default.
• W4387391893 cites W2990946689 @default.
• W4387391893 cites W2998263153 @default.
• W4387391893 cites W3046682791 @default.
• W4387391893 cites W3194169400 @default.
• W4387391893 cites W3199297491 @default.
• W4387391893 cites W4206179351 @default.
• W4387391893 doi "https://doi.org/10.1111/andr.13541" @default.
• W4387391893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37801310" @default.
• W4387391893 hasPublicationYear "2023" @default.
• W4387391893 type Work @default.
• W4387391893 citedByCount "0" @default.
• W4387391893 crossrefType "journal-article" @default.
• W4387391893 hasAuthorship W4387391893A5005900554 @default.
• W4387391893 hasAuthorship W4387391893A5006101864 @default.

• next